Name: John Deere 5220 Tractor

2003-10-11 Planting Planted winter wheat, Pioneer 25R37 untreated seed, on the main site treatment 4 replications 6, 1, 2, 3, and 4. To keep organic certification on treatment 4 we ordered and planted untreated seed. Planted with the JD 5220 tractor and JD 450 conventional 10' drill. Planting depth was 1 inch.

2003-10-13 Planting Planted winter wheat, Pioneer 25R37 untreated seed, on the main site treatment 4 replication 5. To keep organic certification on treatment 4 we ordered and planted untreated seed. Planted with the JD 5220 tractor and JD 450 conventional 10' drill. Planting depth was 1 inch.

2004-03-13 Planting Frost seeded medium red clover at 12 lbs/acre (13.47 kg/ha) into winter wheat on the main site treatment 3, all replications, reps: 3, 1, 2, 4, 5, & 6. Clover was seeded with a John Deere 5220 traveling at 5 mph, gear C1 at 1600 rpm's, and a Gandy Air Seeder. Gandy motor setting was 2.0 and gates were in the middle position. The boom height on the air seeder was 24 inches from the ground. The clover seed was pre-inoculated with Rhizobium Inoculum. Rhizobium is a nitrogen fixing bacteria. [T3, R1-6]

2005-03-16 Planting Frost seeded medium red clover at 9 lbs/acre (10.1 kg/ha) into winter wheat on the main site biodiversity study treatment 3, 6, and 17, all replications. Clover was seeded with a John Deere 5220 traveling at 5 mph, gear C1 at 1600 rpm's, and a Gandy Air Seeder. Gandy motor setting was 2.0 and gates were in the middle position. The boom height on the air seeder was 24 inches from the ground.

2005-06-30 Planting Seeded red clover to treatments 4, 7, and 15 in the biodiverisity study. Seeded the clover with the JD 5220 tractor and grandy air seeder.

2003-10-13 Planting Planted winter wheat, Pioneer 25R37,on the main site treatment 3 replications 6, 1, 2, 3, 4, and 5. Planted with the JD 5220 tractor and JD 450 conventional 10' drill. Planting depth was 1 inch.

2014-04-09 Planting Frost seeded red clover in 2014 LTER Biodiversity Study winter wheat plots. Red clover was seeded into treatment B3 (111, 203, 306, 403), B6 (107, 207, 317, 406), B17 (116, 211, 302, 417) for a total of 12 plots all of which had winter wheat planted in November of 2013. Clover was seeded at 13 lbs/A with Gandy air seeder with a 15' boom. Seeded at 7:30 a.m. - 8:00 a.m. weather conditions at this time were clear and sunny, temperature was 33 degrees F with calm winds. For calibration figures, seed tag information, and other information please see PDF attachment.

2004-03-10 Planting Frost seeded medium red clover at 12 lbs/acre (13.47 kg/ha) into winter wheat on the main site treatment 4, all replications, reps: 6, 1, 2, 3, 4, & 5. Clover was seeded with a John Deere 5220 traveling at 5 mph, gear C1 at 1600 rpm's, and a Gandy Air Seeder. Gandy motor setting was 2.0 and gates were in the middle position. The boom height on the air seeder was 24 inches from the ground. The clover seed was pre-inoculated with Rhizobium Inoculum. Rhizobium is a nitrogen fixing bacteria. [T4, R1-6]

2003-06-30 Mechanical Weed Control Sprayed soybeans on the main site treatment 1 all replications (reps 1, 5, 4, 2, 3, & 6) postemergence for weed control. Basagran (bentazon) was applied at 2 pts/A (2.34 L/ha), Assure II (quizalofop-p-ethyl) was applied at 10 oz/A (0.73 L/ha), and crop oil was applied at 2 pts/A (2.34 L/ha). A total of 3.75 gals of Basagran, 150 oz of Assure II, and 3.75 gals of crop oil were applied on 14.82 acres (6 hectares). Weather Conditions: Air temperature ranging from 75-79 degrees F. Relative Humidity was 43%. Wind was calm. Time of application was between 7:15 p.m. and 8:45 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 25 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Purpose of Application: Control of annual broadleaves and grass weeds. The grass and broadleaves were coming threw the preemergence herbicide that was applied in May of 2003.

2003-06-05 Mechanical Weed Control Sprayed for weeds on the main site treatment 1, all replications, reps. 1, 5, 4, 3, 2, and 6. First Rate (cloransulam-methyl) was applied at 0.6 oz/A, (42 g/ha) and Dual II Magnum (s-metolachlor (+ safener)) was applied at 1.33 pts/A (1.56 L/ha). A total of 9 oz of First Rate and 2.5 gals of Dual II Magnum were applied on 14.82 acres (6 hectares). Weather Conditions: Sunny with air temperature 70 degrees F. Relative Humidity was 40%. Wind was out of the northwest at 3-10 mph averaging 6 mph. Time of application was between 2:30 p.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 30 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Purpose of Application: Control of annual broadleaves and grass weeds. Sprayed preemergence.

2004-04-15 Herbicide Application Sprayed the winter wheat on the main site treatment 1 all replications (reps 6, 3, 2, 4, 5, & 1) for weeds. Purpose of Application: Control of annual broadleaves. Harmony Extra (thifensulfuron methyl + tribenuron methyl) was applied at 0.5 oz/A, (35 g/ha) and NIS surfactant was applied at 0.25%. A total of 7.5 oz of Harmony Extra and 0.75 gal of surfactant were applied on 14.82 acres (6 hectares). Weather Conditions: Sunny with air temperature ranging from 67-72 degrees F. Relative Humidity was ranging from 25%-35%. Wind was out of the west at 0-8 mph's averaging 4 mph's. Time of application was between 5:30 p.m. and 7:15 p.m. Equipment Used: JD 5220 tractor traveling 5.5 mph (gear C1, 1700 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 30 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Sprayed postemergence. Weeds prevalent: Shepherd's-purse (Capsella bursa-pastoris), ranging in height from cotyledon - 3 inches tall, average height 1.5 inches, common chickweed (Stellaria media), ranging in height from cotyledon - 5 inches long, average length 2 inches, henbit (Lamium amplexicaule), ranging in height from cotyledon - 3 inches tall, average height 1 inches.

2004-04-16 Fertilizer Application Applied 28% nitrogen solution (28-0-0) at 16 gals/acre (150L/ha) to all replications of treatment 2 on the LTER main site winter wheat, (reps 2, 3, 4, 5, 6, & 1). Average height of the wheat was 3-4 inches. Total amount of fertilizer applied was 242 gals on 14.82 acres (6 hectares). One gallon of 28% fertilizer weighs 10.67 lbs. 48.78 lbs N/A (54.76 kg N/ha) was applied. Weather Conditions: Mostly sunny with air temperature 61 degrees F. Relative Humidity was 48%. Wind was out of the west-southwest at 0-8 mph's averaging 5 mph's. Time of application was between 10:45 a.m. and 12:15 p.m. Equipment Used: JD 5220 tractor traveling 5.0-6.0 mph?s (gear C1, 1600-1800 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 25-30 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2004-05-06 Herbicide Application Sprayed the winter wheat on the main site lysimeter field for weeds. Purpose of Application: Control of annual broadleaves. Harmony Extra (thifensulfuron methyl + tribenuron methyl) was applied at 0.5 oz/A, (35 g/ha) and NIS surfactant was applied at 0.25%. A total of 3.5 oz of Harmony Extra and 1 qt of surfactant were applied on 6.3 acres of the field (2.55 hectares). The entire field was not sprayed with Harmony Extra. The field area sprayed was from the weather tower towards the west. From the weather tower towards the east was sprayed with 2,4-D on April 30, 2004. Weather Conditions: Mostly cloudy with air temperature 76 degrees F. Relative Humidity was 58%. Wind was out of the west at 0-5 mph averaging 2 mph. Time of application was between 1:15 p.m. and 1:45 p.m. Equipment Used: JD 5220 tractor traveling 5.0-6.0 mph (gear C1, 1600-1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 25-35 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Sprayed postemergence. Weeds prevalent: Shepherd's-purse (Capsella bursa-pastoris), common chickweed (Stellaria media), henbit (Lamium amplexicaule) and dandelion (Taraxacum officinale).

2004-04-15 Herbicide Application Sprayed the winter wheat on the main site treatment T8nt all replications (reps 2, 1, 3, & 4,) for weeds. Purpose of Application: Control of annual broadleaves. Sprayed postemergence. Harmony Extra (thifensulfuron methyl + tribenuron methyl) was applied at 0.5 oz/A, (35 g/ha) and NIS surfactant was applied at 0.25%. I sprayed this after spraying treatment 1 on the main site. After spraying treatment 1 I had around 10-15 gals of material remaining in the sprayer tank, so I used it to spray treatment NT8. Weather Conditions: Sunny with air temperature 67 degrees F. Relative Humidity was 35%. Wind was out of the west at 0-4 mph's averaging 2 mph's. Time of application was between 7:15 p.m. and 7:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 30 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Weeds prevalent: Various

2004-04-10 Herbicide Application Sprayed the winter wheat on the main site treatment 2 all replications (reps 6, 1, 2, 3, 5, & 4) for weeds. Purpose of Application: Control of annual broadleaves. Harmony Extra (thifensulfuron methyl + tribenuron methyl) was applied at 0.5 oz/A, (35 g/ha) and NIS surfactant was applied at 0.25%. A total of 7.5 oz of Harmony Extra and 0.75 gal of surfactant were applied on 14.82 acres (6 hectares). Weather Conditions: Mostly cloudy with air temperature ranging from 48-50 degrees F. Relative Humidity was 45%. Wind was out of the west at 0-8 mph's averaging 3 mph's. Time of application was between 4:00 p.m. and 5:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 25 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Sprayed postemergance. Weeds prevalent: Shepherd's-purse (Capsella bursa-pastoris), ranging in height from cotyledon - 4 inches tall, average height 2 inches, common chickweed (Stellaria media), ranging in height from cotyledon - 8 inches long, average length 3 inches, henbit (Lamium amplexicaule), ranging in height from cotyledon - 4 inches tall, average height 2 inches.

2003-06-06 Mechanical Weed Control Sprayed the European Corn Borer (ECB), in the LTER, preemergence to control annual grass and broadleaves weeds.Dual II Magnum (s-metolachlor (+ safener)) was applied at 1.33 pts/A (1.56 L/ha) and Atrazine 4L was applied at 1 lbs/A (1.56 L/ha). A total of 1 qt of Dual II Magnum and 1.5 qts of Atrazine were applied on the ECB study area. Weather Conditions: Sunny with air temperature 68 degrees F. Wind was out of the southwest at 0-5 mph averaging 2 mph. Time of application was 3:00 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 30 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Purpose of Application: Control of annual broadleaves and grass weeds. Sprayed preemergence.

2003-06-06 Mechanical Weed Control Sprayed the Nitrogen Rate Studies (N-rate) both the irrigated and non-irrigated, in the LTER, preemergence to control annual grass and broadleaves weeds.Dual II Magnum (s-metolachlor (+ safener)) was applied at 1.33 pts/A (1.56 L/ha), Atrazine 4L was applied at 1 lbs/A (1.56 L/ha), and Callisto (mesotrione) was applied at 6 oz/A (0.4 L/ha). A total of 3 qt of Dual II Magnum, 4 qts of Atrazine, and 24 oz of Callisto were applied on the N-rate study area. Weather Conditions: Sunny with air temperature was 68 degrees F. Wind was out of the southwest at 0-5 mph averaging 2 mph. Time of application was between 3:30 p.m. and 5:00 p.m. Equipment Used: JD 5220 tractor traveling 5.5 mph (gear C1, 1700 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 30 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Purpose of Application: Control of annual broadleaves and grass weeds. Sprayed preemergence.

2002-06-29 Herbicide Application Band sprayed for weeds on the main site treatment 3, all replications, reps. 4, 5, 6, 1, 3, and 2. Purpose of Application: Control of annual broadleaves, mostly lambsquarters, velvetleaf, and common ragweed. Sprayed Postemergence. Buctril (bromoxynil) was applied at 1.5 pts/A, (1.76 L/ha). A total of 1.5 gals of Buctril was applied on 7.5 acres (3 hectares). This was a banded application (15 inch band) of weed control. Weather Conditions: air temperature 70-80 degrees F. Relative Humidity was 61%. Wind was out of the southwest at 0-7 mph, averaging 4 mph. Time of application was between 9:00 a.m. and 11:00 a.m. Equipment Used: JD 5220 tractor traveling 4-6 mph with a pull type Top Air sprayer equipped with a PTO driven centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 8003E nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 23 gals/A, (215 L/ha), was used as the carrier.

2003-06-20 Herbicide Application Sprayed the Nitrogen Rate Studies (N-rate), both the irrigated and non-irrigated studies. Roundup Weathermax (glyphosate) was applied at 16 oz/A (1.17 L/ha), and AMS was applied at 17 lbs/100 gals of water. A total of 80 oz of Roundup, and 17 lbs of AMS was applied on the N-rate study area. Weather Conditions: Sunny with air temperature was 74 degrees F. Wind was out of the northeast at 0-8 mph averaging 3 mph. Time of application was between 5:15 p.m. and 5:45 p.m. Equipment Used: JD 5220 tractor traveling 4.5-5.0 mph (gear C1) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 30 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Purpose of Application: Control of volunteer corn (non-roundup ready corn). Sprayed postemergence. Srayed Roundup on both N-rate studies. Because we replanted both of these studies the volunteer corn from the first planting time needed to be reovmed.

2003-06-30 Mechanical Weed Control Sprayed soybeans on the main site treatment 2 all replications (reps 6, 1, 4, 5, 2, & 3) postemergence for weed control. Basagran (bentazon) was applied at 2 pts/A (2.34 L/ha), Assure II (quizalofop-p-ethyl) was applied at 10 oz/A (0.73 L/ha), and crop oil was applied at 2 pts/A (2.34 L/ha). A total of 3.75 gals of Basagran, 150 oz of Assure II, and 3.75 gals of crop oil were applied on 14.82 acres (6 hectares). Weather Conditions: Air temperature was 81 degrees F. Relative Humidity was 41%. Wind was calm. Time of application was between 4:30 p.m. and 6:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 25 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Purpose of Application: Control of annual broadleaves and grass weeds. The grass and broadleaves were coming threw the preemergence herbicide that was applied in May of 2003.

2004-04-16 Fertilizer Application Applied 28% nitrogen solution (28-0-0) at 17 gals/acre (159L/ha) to all replications of treatment T8nt on the LTER main site winter wheat, (reps 2, 1, 3, & 4). Average height of the wheat was 2-3 inches. Total amount of fertilizer applied was 12 gals on 0.7 acre (0.28 hectares). One gallon of 28% fertilizer weighs 10.67 lbs. 51.2 lbs N/A (57.48 kg N/ha) was applied. Weather Conditions: Mostly sunny with air temperature at 77 degrees F. Relative Humidity was 37%. Wind was out of the west-southwest at 0-8 mph's averaging 3 mph's. Time of application was 6:00 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 25-30 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2006-03-15 Planting 3-15-06 Frost seeded medium red clover at 12 lbs/acre (13.5 kg/ha) into winter wheat in the biodiversity study treatment?s 5, 8, and 17, all replications, plot numbers B5: 114, 217, 312, and 405, B8: 118, 214, 310, and 408, B17: 116, 211, 302, and 417. Clover was seeded with a John Deere 5220 tractor traveling at 4 mph, gear C1 at 1300 rpm's, and a Gandy Air Seeder. Gandy motor setting was 1.1 and gates were all the way open. The boom height on the air seeder was 24 inches from the ground. The clover seed was NOT inoculated, pre or at seeding time.

2004-03-15 Planting Frost seeded medium red clover at 12 lbs/acre (13.47 kg/ha) into winter wheat in the biodiversity study treatment's B4 & B7, all replications. Plot numbers B4: (104, 220, 307, & 404); B7: (105, 219, 305, & 407). Clover was seeded with a John Deere 5220 traveling at 5 mph, gear C1 at 1600 rpm's, and a Gandy Air Seeder. Gandy motor setting was 2.0 and gates were in the middle position. The boom height on the air seeder was 24 inches from the ground. The clover seed was pre-inoculated with Rhizobium Inoculum. Rhizobium is a nitrogen fixing bacteria.

2006-05-24 Mechanical Weed Control 5-24-2006 Sprayed for weeds on the main site treatment 2, all replications, reps. 2, 3, 5, 4, 1, and 6. 2, 4-D LV4 (2,4-D Ester) was applied at 1 pt/A (1.2 L/ha), Roundup original Max (glyphosate) was applied at 22 oz/A (1.6 L/ha), and AMS (ammonium sulfate) was applied at 17 lbs/100 gals of water. A total of 2 gals (7.6 L) of 2,4-D was used, and a total of 2.5 gals (9.5 L) of Roundup was applied on 14.82 acres (6 hectares). A total of 51 lbs. of AMS was used in 300 gals of water. Weather Conditions: at 8:30 a.m. Sunny with air temperature 59 degrees F. Relative Humidity was 53%. Wind was out of the northwest at 0-5 mph's averaging 2 mph's. Weather Conditions: at 10:30 a.m. Sunny with air temperature 70 degrees F. Relative Humidity was 36%. Wind was out of the west-southwest at 2-10 mph's averaging 7 mph's. Time of application was between 8:30 a.m. and 10:30 a.m. Equipment Used: JD 5220 tractor traveling 4.0-5.0 mph (gear C1, 1300-1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Purpose of Application: Burndown for no-till soybeans Control of annual broadleaves weeds, grass weeds, and perennial weeds. Sprayed preplant.

2002-06-27 Herbicide Application Stinger was applied postemergence to the corn on the main site treatment 2, all replications (5, 4, 3, 2, 1, and 6). Purpose of application: control of annual sowthistle. This application was a broadcast postemergence herbicide. Stinger (Clopyralid) was applied at 0.5 pt/A, (0.6 L/ha). A total of 1 gal of Stinger was applied on 14 acres (5.67 hectares). Weather Conditions: air temperature 75 degrees F. Wind was out of the northwest at 2-10 mph, with an average of 6 mph. Relative Humidity: 58%. Time of application was between 11:15 a.m. and 1:15 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph, B3 2100 rpm, with a pull type Top Air sprayer equipped with a PTO driven centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Nozzles used were Turbo TeeJet 11003 spaced 15 inches apart with a total boom length of 30 feet. Carrier used: water applied at 20 gals/A (187L/ha). Application pressure: 18-20 psi.

2002-05-24 Herbicide Application Broadcast sprayed T3, all replications (6, 1, 3, 2, 4, and 5) Dual II Magnum was applied preemergence to the corn on the main site treatment 3; Purpose of application: Control of annual grass weeds; This application was a broadcast preemergence herbicide, it was not banded. Most applications in treatment 3 have been banded however this application was broadcast. We did not have the equipment to apply this application in a band so to make sure that we had a preemgence herbicide applied to treatment 3 a broadcast application was made. Dual II Magnum (S-metolachlor (+ safener)) was applied at 1.33 pts/A, (1.56 L/ha). A total of 2.5 gals of Dual II Magnum were applied on 14 acres (5.67 hectares); Weather Conditions: Air temperature: 70 degrees F. Wind was out of the northwest at 0-8 mph, with an average of 5 mph. Relative Humidity: 45%; Time of application: between 2:00 p.m. and 4:00 p.m; Equipment Used: JD 5220 tractor traveling 4.5 mph, gear B3 2000 rpm's, with a pull type Top Air sprayer equipped with a PTO driven centrifugal sprayer pump and a Raven 440 automatic carrier control unit; Nozzles used: Turbo TeeJet 11003 spaced 15 inches apart with a total boom length of 30 feet; Application pressure: 12-14 psi; Carrier used: Water applied at 20 gals/A (187L/ha).

2009-03-13 Planting Frost seeded medium red clover (variety not stated) at 12 lbs/A (13.5 kg/ha) into winter wheat in the Biodiversity Study treatments B5, B8, and B17, all replications. B5 plots: 114, 217, 312, 405; B8 plots: 118, 214, 310, 408; B17 plots: 116, 211, 302, 417. Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length). Gandy motor setting was 1.6 and gates on the Gandy were all the way open. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Total amount of seed used was 11-12 lbs. Each bag of clover is 50 lbs and costs $111.50/bag. Clover seed was pre inoculated with Rhizoblum Inoculant.

2010-11-09 Mechanical Weed Control Finished all spraying of the LTER Main Site alleyways with TRIPLET Selective Herbicide for control of broadleaf weeds in turfgrass. Triplet is composed of the following: Dimethylamine Salt of 2,4-Dichlorophenoxyacetic Acid (30.56%) Dimethylamine Salt of (+)-R-2-(2-Methyl-4-Chlorophenoys) propionic Acid (8.17%) Dimethylamine Salt of Dicamba (3,6-Dichloro-o-anisic Acid) (2.77) other ingredients (58.50%): producedby Nufarm America Inc. 150 Harvester Drive, Burr Ridge, IL 60527. Alleys were sprayed with 2 quarts/A Triplet in 40 gallons/A water. Pressure was kept at 20 psi during application. Weather conditions. Start, 39F, no wind, 72% relative humidity, sunny. End. 66F, 8 mph east wind (10 minute average), 28% relative humidity, sunny. Time of application 9:00 am - 2:30 pm. Total amount sprayed today was 17.5 acres.

2010-04-29 Fertilizer Application Fertilized LTER Main Site Treatments T2 and T1 all replications (order of application was T2R1 T2R2 T2R3 T2R4 T2R5 T2R6, T1R6, T1R1, T1R5, T1R4, T1R2, and T1R3) Applied 28% nitrogen solution (28-0-0) at 11 gals/acre (103 L/ha). Unfertilized microplots which were located in the northwest corner (16.7 feet deep [north to south] and 133 feet long [east to west]) were not fertilized. One gallon of 28% fertilizer weighs 10.67 lbs. so 32.9 lbs N/A (36.8 kg N/ha) was applied. Weather Conditions: Cloudy with air temperature 62 degrees F. Relative Humidity was 42%. Wind was out of the east at 8-13 mphs. Time of application was between 9:00 a.m. and 12:45 p.m. Equipment Used: JD 5220 tractor traveling 5.6 mph (gear C1, 1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 15 psi were used. Purpose of Application: Second half of split application to supply nitrogen fertilizer for the winter wheat.

2010-06-17 Mechanical Weed Control Sprayed herbicide on the GLBRC Main Site Treatment G4 soybeans for post emergence weed control. Roundup Power Max (glyphosate) was applied at 22 oz/A along with Ammonium Sulfate at 17#/100 gallon H20 using 19.2 gallons/A H2O as carrier. Weather conditions: Partly sunny with air temperature of 67 degrees F at start. Relative Humidity as 73%. Wind was calm-5 mph from south (ssw to sse). Time of application was 11:30 a.m. - 4:00 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 19.2 gals/A, (179.5 L/ha), was used as the carrier.

2010-04-20 Mechanical Weed Control Sprayed LTER Main Treatment T1, all replications (reps R1, R5, R4, R2, R3, and R6), for post emergence annual grass and broadleaf weed control in winter wheat. Plots sprayed with OSPREY herbicide at 4.75 oz/A, Preference non-ionic surfactant and anti-foaming agent at 2 qt/100 gal carrier, and Ammonium Sulfate (AMS) at 3 lbs/A. Time of application was 10 a.m. to 12:00 noon. Weather conditions were sunny, temperature was 54F to 59F at end, 50-35% relative humidity, winds out of south by southwest at 3-5 mph with 10 minute average of 2 mph. Equipment used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2004-04-16 Fertilizer Application Applied 28% nitrogen solution (28-0-0) at 16 gals/acre (150L/ha) to all replications of treatment 1 on the LTER main site winter wheat, (reps 2, 3, 4, 5, 1, & 6). Average height of the wheat was 3-4 inches. Total amount of fertilizer applied was 240 gals on 14.82 acres (6 hectares). One gallon of 28% fertilizer weighs 10.67 lbs. 48.38 lbs N/A (54.31 kg N/ha) was applied. Weather Conditions: Mostly sunny with air temperature 76 degrees F. Relative Humidity was 40%. Wind was out of the west-southwest at 0-8 mph's averaging 5 mph's. Time of application was between 1:45 p.m. and 3:15 p.m. Equipment Used: JD 5220 tractor traveling 5.0-6.0 mph?s (gear C1, 1600-1800 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 25-30 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat. ***** Applied 28% nitrogen solution (28-0-0) at 16 gals/acre (150L/ha) to all replications of treatment 3 on the LTER main site winter wheat, (reps 6, 1, 3, 2, 4, & 5). Average height of the wheat was 2-3 inches. Total amount of fertilizer applied was 239 gals on 14.82 acres (6 hectares). One gallon of 28% fertilizer weighs 10.67 lbs. 48.18 lbs N/A (54.09 kg N/ha) was applied. The goal was to apply the nitrogen fertilizer at 3/5 (0.60) rate of the total amount applied to treatments 1 and 2. The protocol was to apply a total amount of 80 lbs N/A for treatments 1 and 2. We applied 32 lbs N/A in the autumn of 2003 so we were trying to apply another 48 lbs N/A in the spring of 2004 on treatments 1 and 2. The 3/5 rate of nitrogen applied to treatment 3 was based on the total annual amount, spring and fall amounts, of nitrogen, not just 3/5 of the nitrogen applied in the spring. Therefore, treatment 3 needed to receive to same amount of nitrogen in the spring as treatments 1 and 2 received, 80 lbs N/A * 0.60 = 48 lbs N/A. Treatment 3 did not receive any nitrogen in 2003. Weather Conditions: Mostly sunny with air temperature ranging from 77-79 degrees F. Relative Humidity was ranging from 30-37%. Wind was out of the west-southwest at 0-8 mph's averaging 3 mph's. Time of application was between 4:00 p.m. and 5:45 p.m. Equipment Used: JD 5220 tractor traveling 5.0-6.0 mph (gear C1, 1600-1800 rpm's) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 25-30 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2010-04-21 Fertilizer Application Fertilized LTER Main Site Treatment T1 all replications (reps. R1, R5,R5, R4, R3, R2, and R6). Applied 28% nitrogen solution (28-0-0) at 15 gals/acre (141L/ha). Unfertilized microplots which were located in the northwest corner (5 meters deep [north to south] and 40 meters long [east to west]) were not fertilized. Total amount of fertilizer applied was 217 gallons on 14.3 acres. One gallon of 28% fertilizer weighs 10.67 lbs. 44.8 lbs N/A (50.12 kg N/ha) was applied. Weather Conditions: Mostly sunny with air temperature 66 degrees F. Relative Humidity was 35%. Wind was out of the west at 7-10 mphs averaging 8 mphs at start. Time of application was between 2:00 p.m. and 3:45 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 10-20 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2010-04-21 Fertilizer Application Fertilized LTER Main Site Treatment T3 all replications (reps. R6, R5, R4, R2, R3, and R1). Applied 28% nitrogen solution (28-0-0) at 15 gals/acre (141L/ha). Unfertilized microplots which were located in the northwest corner (50 feet deep [north to south] and 90 feet long [east to west]) were not fertilized. Total amount of fertilizer applied was 211 gallons on 14.0 acres. One gallon of 28% fertilizer weighs 10.67 lbs. so 44.8 lbs N/A (50.12 kg N/ha) was applied. Weather Conditions: Mostly sunny with air temperature 57 degrees F. Relative Humidity was 44%. Wind was out of the south by southwest at 6 mphs with 10 minute average speed of 4 mphs at start. Time of application was between 10:30 a.m. and 1:00 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 10-20 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2010-04-20 Fertilizer Application Fertilized LTER Main Site Treatment T2 all replications (reps. R2, R3, R4, R5, R1, and R6). Applied 28% nitrogen solution (28-0-0) at 15 gals/acre (141L/ha). Unfertilized microplots which were located in the northwest corner (5 meters deep [north to south] and 40 meters long [east to west]) were not fertilized. Total amount of fertilizer applied was 215 gallons on 14.3 acres. One gallon of 28% fertilizer weighs 10.67 lbs. so 44.8 lbs N/A (50.12 kg N/ha) was applied. Weather Conditions: Mostly sunny with air temperature 62 degrees F. Relative Humidity was 32%. Wind was out of the east at calm to 2 mphs with 10 minute average speed of calm at start. Time of application was between 1:30 p.m. and 3:00 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 10-20 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2008-03-15 Planting Frost seeded medium red clover (variety not stated) at 12 lbs/A (13.5 kg/ha) into winter wheat in the Biodiversity Study treatments B3, B6, and B17, all replications. B3 plots: 111, 203, 306, 403; B6 plots: 107, 207, 317, 406; B17 plots: 116, 211, 302, 417. Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length). Gandy motor setting was 1.6 and gates on the Gandy were all the way open. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Total amount of seed used was 10 lbs. Each bag of clover is 50 lbs and costs $111.50/bag. Clover seed was pre inoculated with Rhizoblum Inoculant.

2010-05-03 Fertilizer Application Fertilized LTER Main Site Lysimeter Field with 28% nitrogen. Applied 28% nitrogen solution (28-0-0) across most of field at 26 gals/acre (westernmost 2/3 of field). The lysimeter field was previously used to help calibrate spray rates for the N-rate study (fertilized the previous week) so some areas were fertilized with undeterminable amounts of nitrogen ranging from 23 lbs/A to approximately 180 lbs/A. One gallon of 28% fertilizer weighs 10.67 lbs. so 77.7 lbs N/A (87.0 kg N/ha) was applied. Weather Conditions: Mostly sunny with air temperature 61 degrees F. Relative Humidity was 63%. Wind was out of the west-southwest at 5-10 mphs at start. Time of application was between 10:30 a.m. and 12:15 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 30 psi were used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2010-05-03 Fertilizer Application Fertilized LTER Main Site Treatment 3 Fertilized Micro-plots, all replications (reps 1, 2, 3, 4, 5, and 6). Microplot area is located in the northwest corner of each replication(50 feet deep [north to south] and 90 feet long [east to west]) and is divided into 6 microplots, each one measures 15' (e-w) x 50' (n-s). Fertilized microplots were the following: T3R1 (1, 3, 4), T3R2 (1, 3, 4), T3R3 (1, 3, 5), T3R4 (3, 4, 6), T3R5 (1, 2, 4), and T3R6 (2, 3, 4). Applied 26 gal/A 28% nitrogen solution (28-0-0) in the microplots; one gallon of 28% fertilizer weighs 10.67 lbs. so 77.7 lbs N/A (87.0 kg N/ha) was applied. Weather Conditions: Mostly sunny with air temperature 61 degrees F. Relative Humidity was 63%. Wind was out of the west-southwest at 5-10 mphs at start. Time of application was between 10:30 a.m. and 12:15 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 15 feet (right side of boom only). 30 psi were used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2010-05-03 Fertilizer Application Fertilized LTER Main Site Treatment 4 Fertilized Micro-plots, all replications (reps 5, 4, 3, 2, 1, and 6). Microplot area is located in the northwest corner of each replication(50 feet deep [north to south] and 90 feet long [east to west]) and is divided into 6 microplots, each one measures 15' (e-w) x 50' (n-s). Fertilized microplots were the following: T4R1 (1, 3, 4), T4R2 (1, 4, 6), T4R3 (2, 4, 5), T4R4 (1, 3, 6), T4R5 (2, 4, 6), and T4R6 (2, 3, 4). Applied 26 gal/A 28% nitrogen solution (28-0-0) in the microplots; one gallon of 28% fertilizer weighs 10.67 lbs. so 77.7 lbs N/A (87.0 kg N/ha) was applied. Weather Conditions: Mostly sunny with air temperature 61 degrees F. Relative Humidity was 63%. Wind was out of the west-southwest at 5-10 mphs at start. Time of application was between 10:30 a.m. and 12:15 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 15 feet (right side of boom only). 30 psi were used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2009-09-23 Mechanical Weed Control Sprayed GLBRC alleyways for weed burndown prior to planting grass seeds. The five westernmost, one northernmost, and two southernmost alleyways were sprayed with Roundup Power Max applied 32 oz/A. Time of application was between 4:00 and 6:00 p.m. Weather conditions were cloudy and overcast, temperature was 70F, 84% relative humidity, winds variable but mostly westerly at 0-3 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2009-09-24 Mechanical Weed Control Sprayed GLBRC Alleyways for weed burndown prior to plant grass seed. The six easternmost and corresponding north-south alleyways were sprayed with Roundup Power Max applied 32 oz/A and ammonium sulfate (AMS) at 17#/100 gallon H2O carrier. A total of 15 quarts of Roundup and 51 pounds of AMS were applied. Time of application was between 12:30 and 2:00 p.m. Weather conditions were cloudy and overcast, temperature was 68F, 87% relative humidity, winds variable but mostly SSE at 0-1 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-05-26 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G5 (all replications, 3, 5, 2, 4, & 1) with 2,4-D Amine 4 herbicide for broadleaf weed control in switchgrass. 2,4-D Amine 4 herbicide (Dimethylamine salt of 2,4-Dichlorophenoxyacetic acid 47.3%) was applied at 32 oz per acre in 20 gallons/A H2O as carrier. Weather conditions: Partly sunny with air temperature of 84 degrees F at start. Relative Humidity as 50%. Wind was calm-5 mph from NNE. Time of application was 12:30-1:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-05-19 Mechanical Weed Control Sprayed the LTER main site, treatment 2, replications 1-6, for weed burndown. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha). Weather conditions: Sunny with air temperature of 60-70 degrees F (15-21°C). Relative Humidity was 48%. Wind was from south southwest at 7 mph. Time of application was 10:00 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 wide angle flat spray nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier

2013-04-22 Mechanical Weed Control Sprayed LTER Main Site Treatment T2 all replications (5, 3, 4, 2, 1, 6), for post emergence weed control in winter wheat. Plots sprayed with Harmony Extra SG herbicide, Active ingredients (Thifensulfuron-methyl 33.33%) and (Tribenuron-methyl 16.67%) at 0.8 oz/A and Preference non-ionic surfactant and anti-foaming agent at 0.25% v/v (1 qt/100 gal carrier). Time of application was 3:30 - 5:00 p.m. Weather conditions were sunny, temperature was 60°F at start and 61°F at end, winds out of the southeast at 2-10 mph with 10 minute average of 5 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Nozzles were spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-04-26 Mechanical Weed Control Sprayed LTER Main Site Fertility Gradient (N-rate) Study, including irrigated and dry-land areas, all replications, for post emergence weed control in winter wheat. Plots sprayed with Affinity BroadSpec herbicide at .8 oz/A and Preference non-ionic surfactant at .25% v/v (1 qt/100 gal carrier). Time of application was 11:30am - 12:20pm. Weather conditions were sunny, temperature was 40°F at start and 54°F at end, 39% relative humidity, winds out of southeast at 6-12 mph with 10 minute average of 8 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2009-07-10 Mechanical Weed Control Sprayed GLBRC ancillary plots on south side of field, soybean trap crop on south and east of fields, soybeans in s/w corner of field area, switchgrass productivity trial plots 304 and 310, switchgrass variety trial soybean filler plots with Roundup Original Max 5.5L at 22 oz/A with ammonium sulfate at 17#/100 gallons (3.4 #/A) for postemergence weed control in soybeans. Time of application was 11:30 a.m. Weather conditions were cloudy, temperature was 77F, 60% relative humidity, winds out of south at 6-8 mph with 10 minute average of 7 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-05-05 Mechanical Weed Control Sprayed GLBRC Main Site replication 6 area, with Roundup Power Max for preplant weed burndown. Roundup was applied at 22 oz/A with ammonium sulfate at 3.4 lbs/A (17 #/100 gallon carrier). Plots first were sprayed with a headland pass (going east/west) on the south 15' and north 15' and then the remaining area was sprayed (going north/south). Time of application was 11:00-1:00 p.m. Weather conditions were partly cloudy, temperature was 63F, 53% relative humidity, winds were calm. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-05-05 Mechanical Weed Control Sprayed GLBRC Main Site treatment G4 with Roundup Power Max for preplant weed burndown. Roundup was applied at 22 oz/A with ammonium sulfate at 3.4 lbs/A (17 #/100 gallon carrier). Plots first were sprayed with a headland pass (going east/west) on the south 15' and north 15' and then the remaining area was sprayed (going north/south). Time of application was 11:00-1:00 p.m. Weather conditions were partly cloudy, temperature was 63F, 53% relative humidity, winds were calm. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-05-06 Mechanical Weed Control Sprayed LTER Main Site Cellulosic Ethanol Study treatment CE3 all replications (reps 1, 2, 3, and 4). Plots sprayed with Roundup Power Max for preplant weed burndown. Roundup was applied at 22 oz/A with ammonium sulfate at 3.4 lbs/A (17 #/100 gallon carrier). Plots were sprayed with a single pass (30' wide) going south to north. Time of application was 8:00-9:15 a.m. Weather conditions were partly cloudy, temperature was 49F, 72% relative humidity, winds were mostly from west southwest from 3-9 mph with 10 minute average of 8 mph. Equipment used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-05-06 Mechanical Weed Control Sprayed LTER Main Site treatment T8nt all replications (reps 1, 2, 3, and 4). Plots sprayed with Roundup Power Max for preplant weed burndown. Roundup was applied at 22 oz/A with ammonium sulfate at 3.4 lbs/A (17 #/100 gallon carrier). Time of application was 8:00-9:15 a.m. Weather conditions were partly cloudy, temperature was 49F, 72% relative humidity, winds were mostly from west southwest from 3-9 mph with 10 minute average of 8 mph. Equipment used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-05-07 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G1 all replications (reps 1, rep 3, rep 2, rep 4 and rep5) for weed burndown and preemergence weed control in corn. Roundup PowerMax (gyphosate) was applied at 22 ounces/acre (1.61 L/ha); and Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) was applied at 3 qts/acre (7.01 L/Ha) with 17 lbs / 100 gallons ammonium sulfate. Plots first were sprayed with a headland pass (going east/west) on the south 10' and north 10' and then the remaining area was sprayed (going north/south). Weather conditions: Cloudy and rainy with air temperature of 49 degrees F (9.4°C). Relative Humidity as 79%. Wind was from NE at 5 mph. Time of application was 6:30-8:00 a.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-06-23 Mechanical Weed Control Sprayed the LTER main site, treatment T3, replications 1-6, for post- emergence weed control of roundup ready soybeans varitey 92Y30, with Roundup OriginalMax 5.5L (glyphosate) at 44 ounces/A with sprayable ammonium sulfate at 17 pounds/100 gallons of water (3.4 pounds/A). Application was broadcast sprayed. Weather conditions at 4 p.m. Sunny with temperatures of 87F, wind was from the SSE at 1 mph. Relative humidity was 51%. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 flat fan nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-05-07 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G3 all replications (reps 1, rep 2, rep 5, rep 3 and rep4) for weed burndown and preemergence weed control in corn. Roundup PowerMax (gyphosate) was applied at 22 ounces/acre (1.61 L/ha); and Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) was applied at 3 qts/acre (7.01 L/Ha) with 17 lbs / 100 gallons ammonium sulfate. Plots first were sprayed with a headland pass (going east/west) on the south 10' and north 10' and then the remaining area was sprayed (going north/south). Weather conditions: Cloudy and rainy with air temperature of 49 degrees F (9.4°C). Relative Humidity as 79%. Wind was from NE at 5 mph. Time of application was 6:30-8:00 a.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2010-05-07 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment corn trap/bait crop on east and south border of field for weed burndown and preemergence weed control in corn. Roundup PowerMax (gyphosate) was applied at 22 ounces/acre (1.61 L/ha); and Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) was applied at 3 qts/acre (7.01 L/Ha) with 17 lbs / 100 gallons ammonium sulfate. Weather conditions: Cloudy and rainy with air temperature of 49 degrees F (9.4°C). Relative Humidity as 79%. Wind was from NE at 5 mph. Time of application was 6:30-8:00 a.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2010-05-26 Mechanical Weed Control Sprayed the GLBRC Main Site treatment plots G6R5 & G6R4 (Miscanthus) and G7R4 (Grass mix) with 2,4-D Amine 4 herbicide for broadleaf weed control. 2,4-D Amine 4 herbicide (Dimethylamine salt of 2,4-Dichlorophenoxyacetic acid 47.3%) was applied at 32 oz per acre in 20 gallons/A H2O as carrier. Weather conditions: Partly sunny with air temperature of 84 degrees F at start. Relative Humidity as 50%. Wind was calm-5 mph from NNE. Time of application was 12:30-1:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-06-24 Mechanical Weed Control Sprayed the LTER main site, treatment 1, reps 1-6, for post-emergence weed control. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha). Weather conditions: Sunny with air temperature of 85-91 degrees F (29-33°C). Relative Humidity was 70%. Wind was from west at 3 mph. Time of application was 10:30 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2010-05-10 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilzed microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. Conditions: Partly sunny with air temperature 58 degrees F. Relative Humidity was 35%. Wind was out of the east at 5-6 mphs. Time of application was between 2:00 p.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 4.2 mph (gear B3, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2010-04-23 Fertilizer Application Fertilized LTER N-rate study (Irrigated and Non-irrigated fertility gradient study). Fertilizer applications were with 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN). A single pass of a boom type sprayer was used broadcast spray and apply the correct amount of fertilizer. The amounts of nitrogen applied were as follows: F1 (101, 203, 209, 404, 501, 603, 709, 804) 0 lbs/A; F2 (102, 202, 304, 406, 502, 602, 704, 806) 20 lbs N/A (7 gal/A); F3 (103, 205, 302, 407, 503, 605, 702, 807) 40 lbs N/A (13.5 gal/A); F4 (104, 208, 305, 403, 504, 608, 705, 803) 60 lbs N/A (20 gal/A); F5 (105, 204, 306, 401, 505, 604, 706, 801) 80 lbs N/A (27 gal/A); F6 (106, 209, 308, 402, 506, 609, 708, 802) 100 lbs N/A (33.5 gal/A); F7 (107, 201, 307, 408, 507, 601, 707, 808) 120 lbs N/A (40 gal/A); F8 (108, 207, 303, 405, 508, 607, 703, 805) 140 lbs N/A (47 gal/A); F9 (109, 206, 301, 409, 509, 606, 701, 809) 160 lbs N/A (53.5 gal/A). Guard plots were sprayed at rates comparable to adjacent treatment plots; i.e. west of 101, unsprayed; west of 301 sprayed at F9 rate; east of 690 sprayed at F6 rate; east of 809 sprayed at F9 rate; middle buffer plots between replications 2 & 5 and between 4 & 7 were sprayed at 80 lbs N/A except for the single plot west of 501 which was unsprayed because it was adjacent to an F1 level. Time of application was between 1:30 and 4:30 p.m. Temperature at start was 61F, 37% relative humidity, skies were cloudy. Wind was between 8-10 mph at start with 10 minute average of 8 mph from ESE. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. The sprayer was controlled by a Raven 440 automatic carrier control unit. Nozzles were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 20-35 psi. TeeJet 8001 nozzles were used for the F2 level. TurboTeeJet 11002 nozzles were used for the F3 and F4 levels. TurboTeeJet 11005 nozzles were used for the F5, F6, F7, F8 and F9 levels.

2010-05-10 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatment G10, MICROPLOT area only, all replications. Treatment G10 microplots were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs / gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas: fertilzed microplot areas 15' wide located on the west side of treatment plots {G10R4, G10R2, G10R3, and G10R5} and located on the east side of the treatment plot G10R1; unfertilized areas which were the remaining 76.86' on the east side of the plots {G10R2, G10R3, G10R4, and G10R5} and located on the west side of treatment plot G10R1. The fertilized 15' microplot received 50 lbs N/A. The unfertilized 76.86' was NOT fertilized. Conditions: Partly sunny with air temperature 58 degrees F. Relative Humidity was 35%. Wind was out of the east at 5-6 mphs. Time of application was between 2:00 p.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 4.2 mph (gear B3, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the microplot treatment areas.

2009-06-23 Mechanical Weed Control Sprayed the LTER main site, treatment T2, replications 1-6 for post-emergence weed control of roundup ready soybeans varitey 92Y30, with Roundup OriginalMax 5.5L (glyphosate) at 22 ounces/A with sprayable ammonium sulfate at 17 pounds/100 gallons of water (3.4 pounds/A). A total of 15 acres were sprayed and 10.3 quarts Roundup and 51 pounds of ammonium sulfate were applied. Weather conditions at 10 a.m. Sunny with temperatures of 74F, wind was calm at 0 mph. Relative humidity was 55%. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 wide angle flat fan nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-05-10 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatment G2, all replications. All treatments were fertilized with 60 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs/gallon so the 60 lbs N/A worked out to 20.0 gal 28%/A. Conditions: Partly sunny with air temperature 58 degrees F. Relative Humidity was 35%. Wind was out of the east at 5-6 mphs. Time of application was between 2:00 p.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2009-05-22 Mechanical Weed Control Sprayed the LTER main site, treatment T6, replications 1-6, for weed burndown. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha). Weather conditions: Partly cloudy with air temperature of 70 degrees F (21°C). Relative Humidity was 41%. Wind was from east northeast at 7 mph. Time of application was 5:30 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-06-22 Mechanical Weed Control Sprayed the LTER main site, treatment 6, replications 1-6, for preplant weed burndown. Roundup Original Max 5.5L (gyphosate) was applied at 22 ounces/acre (0.65 L/ha) with 17 pounds/100 gallons sprayable ammonium sulfate. Weather conditions: Sunny with air temperature of 83-85 degrees F (28-29°C). Relative Humidity was 55%. Wind was from ESE at 6 mph. Time of application was 4:30 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-05-23 Mechanical Weed Control Sprayed the LTER N-rate study, both irrigated and non-irrigated for weed burndown. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha). Weather conditions: Partly cloudy with air temperature of 54 degrees F (12°C). Relative Humidity was 81%. Wind was calm at 0 mph. Time of application was 7:30 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 wide angle flat spray nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier

2009-05-23 Mechanical Weed Control Sprayed the LTER Lysimeter fetch area for weed burndown. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha). Weather conditions: Partly cloudy with air temperature of 54 degrees F (12°C). Relative Humidity was 81%. Wind was calm at 0 mph. Time of application was 7:30 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 wide angle flat spray nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier

2009-05-23 Mechanical Weed Control Sprayed the LTER main site, treatment T8nt, replications 1-4,for weed burndown. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha). Weather conditions: Partly cloudy with air temperature of 54 degrees F (12°C). Relative Humidity was 81%. Wind was calm at 0 mph. Time of application was 7:30 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11033 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier

2009-06-22 Mechanical Weed Control Sprayed the GLBRC main site, treatment G2, replications 1-5, for post-emergence weed control. Roundup Original Max 5.5L (gyphosate) was applied at 22 oz/acre (0.65 L/ha). Weather conditions: Sunny with air temperature of 74 degrees F (23°C). Relative Humidity was 80%. Wind was from south x southeast at 4 mph. Time of application was 9:30 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 wide angle flat spray nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier. Major weeds to be controled were quackgrass and pigweed.

2009-06-15 Mechanical Weed Control Sprayed the LTER main site, European Corn Borer Study (ECB), for preemergence weed control. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) was applied at 3 qts/acre with 17% ammonium sulfate. Weather conditions: Cloudy with air temperature of 74 degrees F (23°C). Relative Humidity was 49%. Wind was from east at 6 mph with a 10 minute average of 7 mph. Time of application was 6:00 pm. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 wide angle flat spray nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-05-23 Mechanical Weed Control Sprayed the LTER Cellulosic Ethanol Study treatment C2 replications 1-4 for weed burndown. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha). Weather conditions: Partly cloudy with air temperature of 54 degrees F (12°C). Relative Humidity was 81%. Wind was calm at 0 mph. Time of application was 7:30 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 wide angle flat spray nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier

2009-06-10 Mechanical Weed Control Sprayed the GLBRC main site, treatment G3 and soybean trap crop, reps 1-5, for post-emergence weed control in ready-to-bolt canola. Roundup Original Max 5.5L (gyphosate) was applied at 11 ounces/acre (0.32 L/ha) with ammonium sulfate at 17#/100gallons. Weather conditions: Partly cloudy with air temperature of 58 degrees F (14°C). Relative Humidity was 85%. Wind was from east southeast at 1 mph. Time of application was 10:00 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-06-05 Mechanical Weed Control Sprayed the GLBRC Switchgrass Variety Trial for preemergence weed control. Drive (quinclorac) at 1 pound/acre and Atrazine 4L at 1/2 pound/acre were applied. Weather conditions: Sunny with air temperature of 74 degrees F (23°C). Relative Humidity was 27%. Wind was from east at 8 mph with a 10 minute average of 6 mph. Time of application was 6:00 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-05-26 Mechanical Weed Control Sprayed the GLBRC Switchgrass Fertility gradient for post emergence weed control. Applied Drive (quinclorac) at 0.5 pounds/acre to the 6 acres of total field. A total of 3 pounds of Drive were applied. Weather conditions: Partly cloudy with air temperature of 72 degrees F (22°C). Relative Humidity was 61%. Wind was from ESE at 6 mph with a 10 minute average of 5 mph. Time of application was 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-05-26 Mechanical Weed Control Sprayed the GLBRC Main Site treatments G5 replications 1-5 for post emergence weed control. Applied Drive (quinclorac) at 0.5 pounds/acre to the 6 acres of total field. A total of 3 pounds of Drive were applied. Weather conditions: Partly cloudy with air temperature of 72 degrees F (22°C). Relative Humidity was 61%. Wind was from ESE at 6 mph with a 10 minute average of 5 mph. Time of application was 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-05-26 Mechanical Weed Control Sprayed the GLBRC Main Site treatment G6 replications 1-5 for post emergence weed control. Applied Drive (quinclorac) at 0.5 pounds/acre to the 6 acres of total field. A total of 3 pounds of Drive were applied. Weather conditions: Partly cloudy with air temperature of 72 degrees F (22°C). Relative Humidity was 61%. Wind was from ESE at 6 mph with a 10 minute average of 5 mph. Time of application was 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-05-26 Mechanical Weed Control Sprayed the GLBRC Miscanthus fertility gradient studies for post emergence weed control. Applied Drive (quinclorac) at 0.5 pounds/acre to the 6 acres of total field. A total of 3 pounds of Drive were applied. Weather conditions: Partly cloudy with air temperature of 72 degrees F (22°C). Relative Humidity was 61%. Wind was from ESE at 6 mph with a 10 minute average of 5 mph. Time of application was 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-05-26 Mechanical Weed Control Sprayed LTER Cellulosic Ethanol treatment C4, C5, C6, & C7 for weed control in switchgrass. Applied Drive (quinclorac) at 0.5 pounds per acre. Weather conditions: Partly cloudy with air temperature of 72 degrees F (22°C). Relative Humidity was 61%. Wind was from ESE at 6 mph with a 10 minute average of 5 mph. Time of application was 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-06-25 Mechanical Weed Control Sprayed the LTER main site, Nitrogen-Rate Study, for post-emergence weed control. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha) and ammonium sulfate (AMS) at 17#/100gal. Weather conditions: Sunny with air temperature of 90 degrees F (33°C). Relative Humidity was 76%. Wind was from northwest at 5 mph. Time of application was between 11:0030 a.m. and 1:00 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-06-25 Mechanical Weed Control Sprayed the LTER Lysimeter fetch area, for post-emergence weed control. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha) and ammonium sulfate (AMS) at 17#/100gal. Weather conditions: Sunny with air temperature of 90 degrees F (33°C). Relative Humidity was 76%. Wind was from northwest at 5 mph. Time of application was between 11:0030 a.m. and 1:00 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2009-05-12 Mechanical Weed Control Sprayed the GLBRC main site, treatment G1 replications 1-5 for weed burndown and preemergence weed control. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha); 2,4-D ester at 16 ounces/acre (1.98 L/ha) and Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) was applied at 3 qts/acre with 17 lbs / 100 gallons ammonium sulfate. Weather conditions: Sunny with air temperature of 66 degrees F (19°C). Relative Humidity was 28%. Wind was from ESE at 5 mph. Time of application was 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2010-03-22 Planting Air seeded LTER Main Site Treatment T4 with Medium Red Clover at 12 lbs/A. Clover seed was from DKB Farms & Services LLC, Columbiaville MI 48421 Lot S 80 100023 DKBCLO8: OCIA Certiified Organic Clover Lot# B1E1-2J3J4A-BJ5O108 (Member ID 11449). Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 20” apart. Gandy motor setting was 1.6 and gates on the Gandy were all the way open. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Temperature was 35'52°F, wind was northeast at 10-13 mph, relative humidity was 46%. All replicates were seeded in 18 passes across the field, replicate order was R6, R5, R3, R1, R2, and R4.

2009-05-12 Mechanical Weed Control Sprayed the GLBRC main site, treatment G4 replications 1-5 for weed burndown and preemergence weed control. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha); 2,4-D ester at 16 ounces/acre (1.98 L/ha) and Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) was applied at 3 qts/acre with 17 lbs / 100 gallons ammonium sulfate. Weather conditions: Sunny with air temperature of 66 degrees F (19°C). Relative Humidity was 28%. Wind was from ESE at 5 mph. Time of application was 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2010-03-19 Planting Airseeded LTER Main Site Biodiversity Study treatments B4, B7, and B17 with Medium Red Clover at 12 lbs/A. Clover seed was from DKB Farms & Services LLC, Columbiaville MI 48421 Lot S 80 100023 DKBCLO8: OCIA Certiified Organic Clover Lot# B1E1-2J3J4A-BJ5O108 (Member ID 11449). Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 20” apart. Gandy motor setting was 1.6 and gates on the Gandy were all the way open. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Temperature was 59°F, wind was from west at 8-10 mph.

2009-05-19 Mechanical Weed Control Sprayed the GLBRC main site, treatment G2, replications 1-5, for weed burndown. Roundup Original Max 5.5L (gyphosate) was applied at 1 quart/acre (3.87 L/ha). Weather conditions: Sunny with air temperature of 60-70 degrees F (15-21°C). Relative Humidity was 35%. Wind was from south southwest at 5 mph. Time of application was 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier

2010-03-19 Planting Airseeded LTER Main Site Treatment T3 with Medium Red Clover at 12 lbs/A. Clover seed was from DKB Farms & Services LLC, Columbiaville MI 48421 Lot S 80 100023 DKBCLO8: OCIA Certiified Organic Clover Lot# B1E1-2J3J4A-BJ5O108 (Member ID 11449). Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 20” apart. Gandy motor setting was 1.6 and gates on the Gandy were all the way open. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. All replicates were seeded in 18 passes across the field, replicate order was R5, R4, R2, R3, R1, and R6.

2010-04-14 Mechanical Weed Control Sprayed LTER Main Site Fertility Gradient (N-rate) Study, including irrigated and dry-land areas, all replications, for post emergence weed control in winter wheat. Plots sprayed with Affinity BroadSpec herbicide at 1.0 oz/A, 2,4-D LV4 herbicide at 12 oz/A and Preference non-ionic surfactant and anti-foaming agent at 0.125% v/v (1 pt/100 gal carrier). Time of application was 3:00-5:00 p.m. Weather conditions were sunny, temperature was 53°F at start and 61°F at end, 39% relative humidity, winds out of southeast at 6-10 mph with 10 minute average of 8 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-04-14 Mechanical Weed Control Sprayed LTER Main Site Lysimeter Area, for post emergence weed control in winter wheat. Plots sprayed with Affinity BroadSpec herbicide at 1.0 oz/A, 2,4-D LV4 herbicide at 12 oz/A and Preference non-ionic surfactant and anti-foaming agent at 0.125% v/v (1 pt/100 gal carrier). Time of application was 3:00-5:00 p.m. Weather conditions were sunny, temperature was 53°F at start and 61°F at end, 39% relative humidity, winds out of southeast at 6-10 mph with 10 minute average of 8 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-04-12 Mechanical Weed Control Sprayed LTER Main Site Treatment T2 all replications for post emergence weed control in winter wheat. Plots sprayed with Affinity BroadSpec herbicide at 1.0 oz/A, 2,4-D LV4 herbicide at 12 oz/A and Preference non-ionic surfactant and anti-foaming agent at 0.125% v/v (1 pt/100 gal carrier). Time of application was 3:00-5:00 p.m. Weather conditions were cloudy, temperature was 63F, 26% relative humidity, winds out of southeast at 7-10 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-04-14 Mechanical Weed Control Sprayed LTER Main Site Treatment T1 all replications, for post emergence weed control in winter wheat. Plots sprayed with Affinity BroadSpec herbicide at 0.8 oz/A, 2,4-D LV4 herbicide at 12 oz/A and Preference non-ionic surfactant and anti-foaming agent at 0.125% v/v (1 pt/100 gal carrier). Time of application was 5:00-7:00 p.m. Weather conditions were sunny, temperature was 71°F at start and 72°F at end, 32/33% relative humidity, winds out of southeast at 7-10 mph with 10 minute average of 8 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-06-17 Mechanical Weed Control Sprayed herbicide on the GLBRC Main Site Treatment soybean trap/border crop for post emergence weed control. Roundup Power Max (glyphosate) was applied at 22 oz/A along with Ammonium Sulfate at 17#/100 gallon H20 using 19.2 gallons/A H2O as carrier. Weather conditions: Partly sunny with air temperature of 67 degrees F at start. Relative Humidity as 73%. Wind was calm-5 mph from south (ssw to sse). Time of application was 11:30 a.m. - 4:00 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 19.2 gals/A, (179.5 L/ha), was used as the carrier.

2010-06-17 Mechanical Weed Control Sprayed herbicide on the LTER Main Site Treatment Interaction Plots (field B3) for post emergence weed control. Roundup Power Max (glyphosate) was applied at 22 oz/A along with Ammonium Sulfate at 17#/100 gallon H20 using 19.2 gallons/A H2O as carrier. Weather conditions: Partly sunny with air temperature of 67 degrees F at start. Relative Humidity as 73%. Wind was calm-5 mph from south (ssw to sse). Time of application was 11:30 a.m. - 4:00 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 19.2 gals/A, (179.5 L/ha), was used as the carrier.

2010-05-05 Mechanical Weed Control Sprayed GLBRC Main Site treatment G2 with Roundup Power Max for postemergence weed control in spring Canola. Roundup was applied at 22 oz/A with ammonium sulfate at 3.4 lbs/A (17 #/100 gallon carrier). Plots first were sprayed with a headland pass (going east/west) on the south 15' and north 15' and then the remaining area was sprayed (going north/south). Time of application was 11:00-1:00 p.m. Weather conditions were partly cloudy, temperature was 63F, 53% relative humidity, winds were calm. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-06-17 Mechanical Weed Control Sprayed herbicide on the LTER Main Site Cellulosic Ethanol Study treatment CE3 soybeans for post emergence weed control. Roundup Power Max (glyphosate) was applied at 22 oz/A along with Ammonium Sulfate at 17#/100 gallon H20 using 19.2 gallons/A H2O as carrier. Weather conditions: Partly sunny with air temperature of 67 degrees F at start. Relative Humidity as 73%. Wind was calm-5 mph from south (ssw to sse). Time of application was 11:30 a.m. - 4:00 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 19.2 gals/A, (179.5 L/ha), was used as the carrier.

2010-06-17 Mechanical Weed Control Sprayed herbicide on the LTER Main Site Treatment T8nt corn for post emergence weed control. Roundup Power Max (glyphosate) was applied at 22 oz/A along with Ammonium Sulfate at 17#/100 gallon H20 using 19.2 gallons/A H2O as carrier. Weather conditions: Partly sunny with air temperature of 67 degrees F at start. Relative Humidity as 73%. Wind was calm-5 mph from south (ssw to sse). Time of application was 11:30 a.m. - 4:00 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 19.2 gals/A, (179.5 L/ha), was used as the carrier.

2010-05-26 Mechanical Weed Control Sprayed the LTER Main Site Treatment Cellulosic Ethanol Study treatments CE1 and CE2 (all replications, 1, 2, 3, & 4) with 2,4-D Amine 4 herbicide mixed with Roundup PowerMax for post emergence weed control in corn. 2,4-D Amine 4 herbicide (Dimethylamine salt of 2,4-Dichlorophenoxyacetic acid 47.3%) was applied at 16 oz per acre and Roundup PowerMax was applied at 32 oz/A in 20 gallons/A H2O as carrier. The treatments correspond to the following plots: CE1 (108, 202, 034, 401) and CE2 (112, 210, 312, 402). Weather conditions: Partly sunny with air temperature of 84 degrees F at start. Relative Humidity as 50%. Wind was calm-5 mph from NNE. Time of application was 2:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2010-05-26 Mechanical Weed Control Sprayed the LTER Main Site Treatment Cellulosic Ethanol Study treatments CE4, CE5, CE6, CE7 and CE8 (all replications, 1, 2, 3, & 4) with 2,4-D Amine 4 herbicide for broadleaf weed control. 2,4-D Amine 4 herbicide (Dimethylamine salt of 2,4-Dichlorophenoxyacetic acid 47.3%) was applied at 32 oz per acre in 20 gallons/A H2O as carrier. The treatments correspond to the following plots: CE4 (110, 203, 302, 404), CE5 (106, 205, 303, 405), CE6 (111, 204, 310 406), CE7 (107, 208, 309, 407) and CE8 (103, 211, 307, 408). Weather conditions: Partly sunny with air temperature of 84 degrees F at start. Relative Humidity as 50%. Wind was calm-5 mph from NNE. Time of application was 1:30-2:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2010-08-16 Mechanical Weed Control Sprayed the LTER Main Site T1 (replications R6 & R5), T2 (replications R3, R6, R1, and R5), with Roundup (32 oz/A) + AMS (3.4 #/A). Weather conditions at time of application: Sunny, 69F winds from the west at 6 mph, 67% relative humidity. Applied using a JD5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) pulling a Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom lenght of 30 feet. 20-30 psi were used. Water was applied at 20 gal/A as carrier.

2010-11-08 Mechanical Weed Control Sprayed LTER Main Site alleyways with TRIPLET Selective Herbicide for control of broadleaf weeds in turfgrass. Triplet is composed of the following: Dimethylamine Salt of 2,4-Dichlorophenoxyacetic Acid (30.56%) Dimethylamine Salt of (+)-R-2-(2-Methyl-4-Chlorophenoys) propionic Acid (8.17%) Dimethylamine Salt of Dicamba (3,6-Dichloro-o-anisic Acid) (2.77) other ingredients (58.50%): producedby Nufarm America Inc. 150 Harvester Drive, Burr Ridge, IL 60527. Alleys were sprayed with 2 quarts/A Triplet in 40 gallons/A water. Pressure was kept at 20 psi during application. Weather conditions. Start, 42F, no wind, 64% relative humidity, sunny. End. 64F, 1 mph south wind, 32% relative humidity, sunny. Time of application 10:00 am - 3:30 pm.

2010-05-20 Fertilizer Application Fertilized GLBRC Main Site Switchgrass Fertility Study (n-rate), all treatments and all replications. Fertilizer applied as 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN). Applied with a Top-Air Sprayer with a 15' boom length. A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. The amounts of nitrogen applied were as follows: SWF1 (108, 201, 307, 408) 0 lbs/A; SWF2 (104, 206, 308, 407) 25 lbs N/A (8.4 gal/A); SWF3 (102, 202, 302, 406) 50 lbs N/A (16.7 gal/A); SWF4 (105, 208, 305, 405) 75 lbs N/A (25.1 gal/A); SWF5 (101, 205, 304, 404) 100 lbs N/A (33.5 gal/A); SWF6 (107, 207, 301, 403) 125 lbs N/A (41.8 gal/A); SWF7 (106, 204, 306, 402) 150 lbs N/A (50.2 gal/A); SWF8 (103, 203, 303, 401) 175 lbs N/A (58.6 gal/A). Time of application was between 3:00 and 4:30 p.m. Temperature at start was 79F, 43% relative humidity, skies were clear and sunny. Wind was between 0-3 mph SSE. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. The sprayer was controlled by a Raven 440 automatic carrier control unit. Nozzles were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 20-35 psi. No passes were made through the SWF1 treatment area. TeeJet 8001 nozzles were used for the SWF2 and SWF3 level. TurboTeeJet 11003 nozzles were used for the SWF4, SWF5 and SWF6 levels. TurboTeeJet 11005 nozzles were used for the SWF7 and SWF8 levels.

2010-05-10 Fertilizer Application Sprayed fertilizer in the LTER Main Site Cellulosic Ethanol Study treatments CE4 Swithchgrass (Cave-in-rock)+N (101, 203, 302, 404) and CE6 Swithcgrass (Southlow)+N (111, 204, 310, 406) with 50# N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 #/gallon so the 50# N/A worked out to 16.7 gal 28%/A. Plots were sprayed with a single pass of the tractor/sprayer down the center of the plot. Weather Conditions: Partly sunny with air temperature 58 degrees F. Relative Humidity was 35%. Wind was out of the east at 5-6 mphs. Time of application was between 2:00 p.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 4.2 mph (gear B3, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2010-05-06 Mechanical Weed Control Sprayed LTER Interaction Study Site, all 16 plots. Plots sprayed with Roundup Power Max for preplant weed burndown and removal of winter wheat. Roundup was applied at 22 oz/A with ammonium sulfate at 3.4 lbs/A (17 #/100 gallon carrier). Plots were sprayed with two 30' wide passes followed by a single 10' wide pass on the west side. An area approximately 15' wide on the east side of the field was not sprayed. Where lysimeters were located, they were sprayed by running the boom of the sprayer over the top of the entire lysimeter area and spraying them at the normal field rate listed above. Time of application was 8:00-9:15 a.m. Weather conditions were partly cloudy, temperature was 49F, 72% relative humidity, winds were mostly from west southwest from 3-9 mph with 10 minute average of 8 mph. Equipment used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2011-05-02 Mechanical Weed Control Sprayed the LTER Main Site Treatment T2 (no-till) plots, all replications (reps 2, 3, 4, 5, 1 and 6) with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for preplant weed burndown. Roundup Power Max was applied at 44 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. Equipment used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2011-05-02 Mechanical Weed Control Sprayed the LTER Main Site Lysimeter Field with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for preplant weed burndown. Roundup Power Max was applied at 44 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. Microplots located in the northwest corner of each plot were not sprayed. The microplots measured 50 feet (north-south) by 133' (east-west). Equipment used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2011-05-03 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G1 (replicates 2, 5, 4, 3, and 1) with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for preplant weed burndown. Roundup Power Max was applied at 44 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. Equipment used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2011-05-03 Mechanical Weed Control Sprayed the LTER Main Site Treatment Fertility Gradient and Irrigated Fertility Gradient [N-Rate] Studies, all blocks and all treatments with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for preplant weed burndown. Roundup Power Max was applied at 44 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. The field was sprayed starting in the southeast corner. A single headland pass was made on both the north and the south side of the field. Because of the 15' treatment plot widths, the drive paths were centered between treatments so that we were not driving through the center of the treatment plots. Equipment used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2011-05-03 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G2 (replicates 1, 2, 5, 3, and 4) with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for preplant weed burndown. Roundup Power Max was applied at 44 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. Equipment used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2010-08-18 Mechanical Weed Control Sprayed the LTER Main Site N-rate study (irrigated and non-irrigated fertility gradient) and the Lysimeter field with Roundup (32 oz/A) + AMS (3.4 #/A). Weather conditions at time of application: Sunny, approximately 65F, winds calm. Applied using a JD5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) pulling a Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom lenght of 30 feet. 20-30 psi were used. Water was applied at 20 gal/A as carrier.

2010-08-17 Mechanical Weed Control Sprayed the LTER Main Site T1 (replications R1, R4, R2 & R3), T2 (replications R2 & R4), with Roundup (32 oz/A) + AMS (3.4 #/A). Weather conditions at time of application: Sunny, 65F winds from the southwest at 1 mph, 84% relative humidity. Applied using a JD5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) pulling a Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom lenght of 30 feet. 20-30 psi were used. Water was applied at 20 gal/A as carrier.

2010-08-18 Mechanical Weed Control Sprayed the GLBRC Main Site treatment G2 (all replications, R3, R2, R5, R1, and R4) with Roundup (32 oz/A) + AMS (3.4 #/A). Weather conditions at time of application: Sunny, approximately 65F, winds calm. Applied using a JD5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) pulling a Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom lenght of 30 feet. 20-30 psi were used. Water was applied at 20 gal/A as carrier.

2011-05-03 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G4 (replicates 1, 2, 4, 3, and 5) with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for preplant weed burndown. Roundup Power Max was applied at 44 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. Equipment used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2011-05-09 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G1, continuous corn, all replications (reps. 4, 3, 1, 5, and 2) for preemergence weed control in corn. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] was applied at 3 qts/acre (7.01 L/Ha). Weather conditions: Sunny with air temperature of 62 degrees F (16.7 degrees C). Relative Humidity was 46%. Wind was from E at 10 mph. Time of application was 10:30 a.m. - 12:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-09 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G2, rotational corn, all replications (reps. 5, 2, 3, 4, and 1) for preemergence weed control in corn. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] was applied at 3 qts/acre (7.01 L/Ha). Treatment G2 had headland passes made on the N and S side of the plots first which were approximately 10' wide, followed by spraying the main plot area in three passes of the sprayer. Weather conditions: Sunny with air temperature of 62 degrees F (16.7 degrees C). Relative Humidity was 46%. Wind was from E at 10 mph. Time of application was 10:30 a.m. - 12:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-09 Mechanical Weed Control Sprayed the LTER Main Site Treatment T2 all replications (reps. 5, 2, 3, 4, 1 and 6) for preemergence weed control in corn. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] was applied at 3 qts/acre (7.01 L/Ha). No-herbicide microplots (last 33.3' from north) in northwest corner of treatment plots were not sprayed. Weather conditions: Sunny with air temperature of 49 degrees F (9.7 degrees C). Relative Humidity was 64%. Wind was from NE at 4 mph. Time of application was 8:15 a.m. - 10:00 a.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-13 Mechanical Weed Control Sprayed the GLBRC Main Site Ancillary Plot Area (”Replicate 6” plots) and southern trap crop area for weed burn down. Sprayed with Roundup Power Max (Glyphosate EPA 524-549) at 44 oz/A, 2,4-D LV4 (ester of 2,4-D, EPA 1381-102) at 16 oz/A, and Ammonium Sulfate (CAS number: 7783-20-2) at 3.4 pound/A in 20 gallons/A of water. Weather conditions: Partly sunny with air temperature of 81 degrees F (27.2 degrees C). Relative Humidity was 63%. Wind was from N at 6 mph. Time of application was 2:45 p.m. - 3:30 a.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-12 Mechanical Weed Control Sprayed the LTER Main Site Cellulosic Ethanol Study treatments CE1 (plots 108, 202, 304, 401), CE2 (plots 112, 210, 312, 402), and CE3 (plots 105, 207, 301, 403) with Roundup Power Max (glyphosate [EPA 524-549]) at 44 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 pound/A in 20 gallons/A of water. Plots were sprayed with the full width of the spray boom in a single 30' wide pass centered on the width of the plot. Weather conditions: Sunny to partly sunny with air temperature of 72 degrees F (22.2 degrees C) at start. Relative Humidity was 71%. Wind was from S at 2 mph. Time of application was 11 a.m. - 12:01 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-12 Mechanical Weed Control Sprayed the LTER Treatment T8nt, all replications, with Roundup Power Max (glyphosate [EPA 524-549]) at 44 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 pound/A in 20 gallons/A of water. Headland passes were made on the N and S side of the plots prior to spraying the main plot areas. Weather conditions: Sunny to partly sunny with air temperature of 72 degrees F (22.2 degrees C) at start. Relative Humidity was 71%. Wind was from S at 2 mph. Time of application was 11 a.m. - 12:01 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi was used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-12 Mechanical Weed Control Sprayed the GLBRC Main Site Treatments G3, all replications, (reps 2, 3, 5, 4, and 1) and the bait crop areas on the S and E of the field, with Roundup Power Max (glyphosate [EPA 524-549]) at 44 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 pound/A in 20 gallons/A of water. Treatment G3 had headland passes made on the N and S side of the plots first which were approximately 10' wide, followed by spraying the main plot area in three passes of the sprayer. Weather conditions: Sunny to partly sunny with air temperature of 72 degrees F (22.2 degrees C) at start. Relative Humidity was 71%. Wind was from S at 2 mph. Time of application was 1 p.m. - 2:00 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-11 Mechanical Weed Control Sprayed the LTER Main Site Fertility Gradient Study, both irrigated and rainfed, and also the Lysimeter field, for preemergence weed control in corn. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] was applied at 3 qts/acre (7.01 L/Ha). For the Lysimeter field, the last pass to the N was centered on the alleyway between T4R6 and T6R6, which puts the N edge of the field 15 feet to the N of that centerline. Weather conditions: Sunny to partly sunny with air temperature of 80 degrees F (27 degrees C) at start. Relative Humidity was 55%. Wind was from NE at 5 mph. Time of application was 1:30 p.m. - 4:00 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-11 Mechanical Weed Control Sprayed the LTER Main Site Treatment T1, all replications (reps. 6, 5, 1, 4, 2, and 3) for preemergence weed control in corn. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] was applied at 3 qts/acre (7.01 L/Ha). Weather conditions: Sunny with air temperature of 59-77 degrees F (15 - 25 degrees C). Relative Humidity was 60-62%. Wind was from E at 2 - 4 mph. Time of application was 7:15 a.m. - 11:15 a.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-12 Mechanical Weed Control Sprayed the LTER Interaction Field (Identified as field B3 at the Farming Systems Center) with Roundup Power Max (glyphosate [EPA 524-549]) at 44 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 pound/A in 20 gallons/A of water. All 16 plots of Interaction Field were sprayed; however, weedy areas corresponding to approximately 15' on the E side of each plot were left unsprayed. All plots had headland passes made on both the N and S side prior to spraying the many area of the plots. Weather conditions: Sunny to partly sunny with air temperature of 72 degrees F (22.2 degrees C) at start. Relative Humidity was 71%. Wind was from S at 2 mph. Time of application was 11 a.m. - 12:01 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-13 Mechanical Weed Control Sprayed the GLBRC Main Site Ancillary Plot Area ”Brook House Field” for weed burn down. Sprayed with Roundup Power Max (Glyphosate EPA 524-549) at 44 oz/A, 2,4-D LV4 (ester of 2,4-D, EPA 1381-102) at 16 oz/A, and Ammonium Sulfate (CAS number: 7783-20-2) at 3.4 pound/A in 20 gallons/A of water. Weather conditions: Partly sunny with air temperature of 81 degrees F (27.2 degrees C). Relative Humidity was 63%. Wind was from N at 6 mph. Time of application was 2:45 p.m. - 3:30 a.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-05-18 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatment G10, MICROPLOT area only, all replications (replications 4, 1, 2, 3, 5). Treatment G10 microplots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs / gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas: fertilized microplot areas 15' wide located on the west side of treatment plots [G10R2, G10R3, G10R4, and G10R5] and located on the east side of the treatment plot G10R1; unfertilized areas which were the remaining 76.86' on the east side of the plots [G10R2, G10R3, G10R4, and G10R5] and located on the west side of treatment plot G10R1. The fertilized 15' microplot received 50 lbs N/A. The unfertilized 76.86' was NOT fertilized. Conditions: Cloudy with air temperature 53 degrees F. Relative Humidity was 93%. Wind was out of the NE at 5-7 mphs. Time of application was between 9:00 a.m. and 11:30 a.m. Equipment Used: JD 5220 tractor traveling 3.4 - 3.7 mph (gear B3, 1450 - 1600 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the microplot treatment areas.

2011-05-18 Fertilizer Application Fertilized the GLBRC Main Site Treatments G5, Switchgrass, all replications (replications 5, 4, 1, 2, and 3). All plots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilized microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. In all plots, for the first pass of the sprayer, only half the boom width (15') was used and a pass was made S to N along the W side of the fertilized area. The second and third passes were made with the full boom width (30'), with the second pass being S to N along the E side of the fertilized area and the third pass being N to S in the middle of that area. Conditions: Cloudy with air temperature 53 degrees F (11.7 degrees C). Relative Humidity was 93%. Wind was out of the east at 5 - 7 mphs. Time of application was between 9:00 a.m. and 11:30 a.m. Equipment Used: JD 5220 tractor traveling 3.4 - 3.7 mph (gear B3, 1450 - 1600 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2011-05-18 Fertilizer Application Fertilized the GLBRC Main Site Treatments G6, Miscanthus, all replications (replications 5, 4, 1, 2, and 3). All plots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilized microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. In all plots, for the first pass of the sprayer, only half the boom width (15') was used and a pass was made S to N along the W side of the fertilized area. The second and third passes were made with the full boom width (30'), with the second pass being S to N along the E side of the fertilized area and the third pass being N to S in the middle of that area. Conditions: Cloudy with air temperature 53 degrees F (11.7 degrees C). Relative Humidity was 93%. Wind was out of the east at 5 - 7 mphs. Time of application was between 9:00 a.m. and 11:30 a.m. Equipment Used: JD 5220 tractor traveling 3.4 - 3.7 mph (gear B3, 1450 - 1600 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2011-05-18 Fertilizer Application Fertilized the GLBRC Main Site Treatments G7, Grass mix, all replications (replications 5, 4, 1, 2, and 3). All plots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilized microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. In all plots, for the first pass of the sprayer, only half the boom width (15') was used and a pass was made S to N along the W side of the fertilized area. The second and third passes were made with the full boom width (30'), with the second pass being S to N along the E side of the fertilized area and the third pass being N to S in the middle of that area. Conditions: Cloudy with air temperature 53 degrees F (11.7 degrees C). Relative Humidity was 93%. Wind was out of the east at 5 - 7 mphs. Time of application was between 9:00 a.m. and 11:30 a.m. Equipment Used: JD 5220 tractor traveling 3.4 - 3.7 mph (gear B3, 1450 - 1600 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2011-05-18 Fertilizer Application Fertilized the GLBRC Main Site Treatments G9, Old-field, all replications (replications 5, 4, 1, 2, and 3). All plots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilized microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. In all plots, for the first pass of the sprayer, only half the boom width (15') was used and a pass was made S to N along the W side of the fertilized area. The second and third passes were made with the full boom width (30'), with the second pass being S to N along the E side of the fertilized area and the third pass being N to S in the middle of that area. Conditions: Cloudy with air temperature 53 degrees F (11.7 degrees C). Relative Humidity was 93%. Wind was out of the east at 5 - 7 mphs. Time of application was between 9:00 a.m. and 11:30 a.m. Equipment Used: JD 5220 tractor traveling 3.4 - 3.7 mph (gear B3, 1450 - 1600 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2011-05-20 Fertilizer Application Fertilized the LTER Cellulosic Biofuels Experiment, treatments C4 Switchgrass (Cave-in-rock + N) (reps 110, 203, 302, and 404) and C6 Switchgrass (Southlow + N) (reps 111, 204, 310, and 408). All plots were fertilized with 50 lbs N/A applied as 28% N fertilizer (UAN) which is comprised of Urea + Ammonium Nitrate (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. All treatment plots were sprayed in one pass of the sprayer with the outside two nozzles shut off leaving the boom width, from outside to outside nozzles, of 27.5'. Conditions: Cloudy with air temperature approximately 63 degrees F (18.3 degrees C). Relative Humidity was approximately 90%. Wind was calm. Time of application was between 11:15 and 11:59 a.m. Equipment Used: JD 5220 tractor traveling 2.7 mph (gear B3, 1450) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2011-05-20 Fertilizer Application Fertilized the GLBRC Main Site Treatment G4, rotational Canola, all replications (replications 5, 4, 3, 2, and 1). All plots were fertilized with 80 lbs N/A applied as 28% N fertilizer (UAN) which is comprised of Urea + Ammonium Nitrate (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs /gallon so the 80 lbs N/A worked out to 26.7 gal 28%/A. Most of the canola plants were still at the cotyledon stage with the first leaves barely starting to become visible. In all plots, the first pass of the sprayer was made S to N along the W side of the plot. The second pass being N to S along the E side of the plot and the third pass being S to N in the middle of the plot. Conditions: Cloudy with air temperature approximately 63 degrees F (18.3 degrees C). Relative Humidity was approximately 90%. Wind was calm. Time of application was between 10:30 a.m. and 11:15 a.m. Equipment Used: JD 5220 tractor traveling 2.3 mph (gear B3, 1400 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2011-05-20 Fertilizer Application Fertilized GLBRC Main Site Switchgrass Fertility Study (SWF: N-rate), all treatments and all replications. Fertilizer applied as 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN) (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate).The amounts of nitrogen applied were as follows: SWF1 (108, 201, 307, 408) 0 lbs/A; SWF2 (104, 206, 308, 407) 25 lbs N/A (8.4 gal/A); SWF3 (102, 202, 302, 406) 50 lbs N/A (16.7 gal/A); SWF4 (105, 208, 305, 405) 75 lbs N/A (25.1 gal/A); SWF5 (101, 205, 304, 404) 100 lbs N/A (33.5 gal/A); SWF6 (107, 207, 301, 403) 125 lbs N/A (41.8 gal/A); SWF7 (106, 204, 306, 402) 150 lbs N/A (50.2 gal/A); SWF8 (103, 203, 303, 401) 175 lbs N/A (58.6 gal/A). Time of application was between 9:00 - 10:30 a.m. Temperature at start was approximately 55F, 90% relative humidity, skies were cloudy. Wind was calm. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. The sprayer was controlled by a Raven 440 automatic carrier control unit. Nozzles were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 15-20 psi. No passes were made through the SWF1 treatment area. TeeJet 8001 nozzles were used for the SWF2 level. TurboTeeJet 11003 nozzles were used for the SWF3, SWF4 and SWF5 levels. TurboTeeJet 11005 nozzles were used for the SWF6, SWF7 and SWF8 levels.

2011-06-21 Mechanical Weed Control Sprayed Roundup Power Max and Ammonium Sulfate on fields adjacent to the GLBRC Main Site. Fields which were sprayed were the Soybeans to the north of the ”Brook House”, the center row of the trap crop corn on the east side of the field, the trap crop soybeans on the east side of the field and the trap crop soybeans on the south side of the field. Roundup Power Max (glyphosate [EPA 524-549]) at 22 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 lbs/A. The spray time was 2:15 - 3:30 p.m. Weather conditions were sunny, 85 degrees F, 77% relative humidity and wind at 0-3 mph from the SE. Applied with the John Deere 5220 tractor pulling a Top Air Sprayer equipped with a hydraulic centrifugal pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 apart with a total boom length of 30 feet. 20 p.s.i were used and water was used as a carrier at 20 gallons/A

2011-06-20 Mechanical Weed Control Sprayed the INTERACTION Field (FSC field B-3), all plots, for postemergence weed control in corn. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] was applied at 3 qts/acre (7.01 L/Ha), Roundup Power Max (glyphosate [EPA 524-549]) at 22 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 lbs/A. Fields were sprayed N-S directions with 7' wide headland passes being made on E and W sides. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier. Weather conditions: 70 degrees F, 85% relative humidity, winds from SE at 2-7 mph. Time of application: 1 p.m. - 4 p.m.

2011-06-21 Mechanical Weed Control Sprayed LEXAR on the following fields: Lysimeter field, north end only, from alley to the south of the T2R6 plot up to the northern alley; FSC field B-0; GLBRC Main Site replicate 6 plots, the 4th, 5th, and 6th plots from the west end of replicate 6, LTER Main Site Cellulosic Ethanol, plots CE301, CE401, and CE403. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] was applied at 2.5 qts/acre (5.84 L/Ha). Weather conditions were sunny, temperatures of 82-85 degrees F, wind was variable 0-6 mph from the S-SE, relative humidity was 80-77%. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-06-20 Mechanical Weed Control Sprayed the LTER Treatment T8nt, all replications (reps. 1, 2, 3, and 4) for postemergence weed control in corn. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] was applied at 3 qts/acre (7.01 L/Ha), Roundup Power Max (glyphosate [EPA 524-549]) at 22 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 lbs/A. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier. Weather conditions: 70 degrees F, 85% relative humidity, winds from SE at 2-7 mph. Time of application: 1 p.m. - 4 p.m.

2011-06-17 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G3, all replications, (reps. 4, 3, 5, 2, and 1) for postemergence weed control in soybeans. Roundup Power Max (glyphosate [EPA 524-549]) at 22 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 lbs/A was applied. Equipment Used: JD 5220 tractor traveling approximately 5.0 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-06-17 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G4, all replications, (reps. 4, 5, 3, 2, and 1) for postemergence weed control in canola. Roundup Power Max (glyphosate [EPA 524-549]) at 22 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 lbs/A was applied. Equipment Used: JD 5220 tractor traveling approximately 4.6 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-06-17 Mechanical Weed Control Sprayed the trap crops at the GLBRC Main Site for preemergence weed control in non-Roundup Ready corn. Areas sprayed were the 3 corn plots in Replicate 6 and the north end trap crop. Roundup Power Max (glyphosate [EPA 524-549]) at 22 oz/A and Ammonium Sulfate (CAS 7783-20-2) at 3.4 lbs/A was applied. Weather conditions: Sunny, 78 degrees F, 62% relative humidity, wind at 6 mph from NE. Time: 1:30 - 4:30 p.m. Equipment Used: JD 5220 tractor traveling approximately 5.0 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-06-17 Mechanical Weed Control Sprayed the Lysimeter Field at the LTER Main Site with Roundup Power Max and Ammonium Sulfate. Only the north 1/2 of the field was sprayed, starting even with the south end of T2R6 and going to the north. The field was sprayed for postplant, preemergence weed control in corn. Weather conditions: Sunny with air temperature of 78 degrees F at start. Relative Humidity was 62%. Wind was from NE at 6 mph. Time of application was 1:30 p.m. - 4:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-06-21 Mechanical Weed Control Sprayed the Cellulosic Ethanol Study, corn treatments, all replications CE1 (plots 108, 202, 304, and 401) and CE3 (plots 105, 207, 301, and 403) for postemergence weed control in corn. Plots were sprayed over several days with Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] at 3 qts/acre (7.01 L/Ha), Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) at 22 oz/A, and Ammonium Sulfate (sprayable, CAS #7783-20-2) at 3.4 lbs/A with the Lexar being applied either solo or as a tank mix with the Roundup + AMS, depending on what was available in the spray tank at that time. Spray dates were 6/17, 6/20 and 6/21. All plots received the final spray amounts of Lexar at 3 qts/A, Roundup PowerMax at 22 oz/A and AMS at 3.4 lbs/A. Weather conditions depended on the day. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-06-21 Mechanical Weed Control Sprayed the Cellulosic Ethanol Study, corn treatments, all replications CES1 (plots 108, 202, 304, and 401) and CES3 (plots 105, 207, 301, and 403) for postemergence weed control in corn. Plots were sprayed over several days with Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] at 3 qts/acre (7.01 L/Ha), Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) at 22 oz/A, and Ammonium Sulfate (sprayable, CAS #7783-20-2) at 3.4 lbs/A with the Lexar being applied either solo or as a tank mix with the Roundup + AMS, depending on what was available in the spray tank at that time. Spray dates were 6/17, 6/20 and 6/21. All plots received the final spray amounts of Lexar at 3 qts/A, Roundup PowerMax at 22 oz/A and AMS at 3.4 lbs/A. Weather conditions depended on the day. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2011-06-17 Mechanical Weed Control Sprayed the LTER Main Site treatment T3, all replications (reps 1, 3, 2, 4, 5 and 6), for post emergence weed control in corn. Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] was applied at 3 qts/acre, Roundup Power Max (glyphosate [EPA 524-549]) at 22 oz/A, and Ammonium Sulfate (CAS 7783-20-2) at 3.4 pound/A in 20 gallons/A of water. Weather conditions: Partly sunny, 80 degrees F, 55% relative humidity, winds NE at 3 mph. Time of application: 6:00 p.m. - 8:00 p.m.

2011-07-07 Mechanical Weed Control Sprayed the T3, LTER Main Site, Treatment T3 ”Business As Usual” [BAU] Microplots (replicates 6, 5, 4, 3, 2 and 1) for post emergence weed control in corn. The microplots which were sprayed were the ”BAU+Fertilization” and the ”BAU-Fertilization” microplots located in the northwest corner of the treatment plot. Each microplot is 15' wide (six rows of corn) and 50' long. Plots were sprayed with Dual II Magnum (S-metolachlor 82.4%) [EPA Reg. No. 100-818], Roundup PowerMax (Glyphosate 48.7%) [EPA Reg. No. 524-549] and Ammonium Sulfate [CAS 7783-20-2]. Dual II Magnum was applied at 16 oz/A, Roundup Power Max at 32 oz/A and Ammonium Sulfate at 3.4 lbs/A. This herbicide combination was selected to best fit with the herbicide combination applied earlier to the rest of the treatment, and take into consideration the current weed pressure. Weather conditions at the start (9:30 a.m.) Sunny-partly overcast, 67 degrees F, 69% relative humidity, winds 2-6 mph from NE. Equipment Used: JD 5220 tractor traveling 4.4 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 13.75 feet (11 nozzles). 20 psi were used. Water applied at 25 gals/A was used as the carrier.

2012-06-20 Mechanical Weed Control Sprayed the GLBRC treatment G4, weed control in soybeans. Sprayed using Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 22 oz/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions at start: Sunny with air temperature of 78 degrees F. Relative Humidity was 64%. Wind was from the southwest at 5 mph. Time of application was 10-11 a.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-04-27 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G1, all replications (reps. 1, 3, 2, 5 and 4) for preplant burndown weed control using a tank mix of Roundup PowerMax, 2,4-D LV4, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 (EPA Reg. No. 1381-102) (2-ethylhexyl ester of 2,4-dichlorophenoxyacetic acid 66.2%) was applied at 1 pt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 42 degrees F (5.6 degrees C). Relative Humidity was 50%. Wind was from north at 2 mph. Time of application was 10:30 a.m. - 2:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-04 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilzed microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer; the first pass was centered 15' from the west edge of the main plot, but half the boom (over the microplot area) was shut off. Conditions: Cloudy with air temperature 68 degrees F. Relative Humidity was 83%. Wind was calm, but out of the south at 0 mphs. Time of application was between 11:30 a.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 3.5 mph (gear B2, 1650 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11002 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2012-04-27 Mechanical Weed Control Sprayed the GLBRC Main Site trap crop area (east, north, west and east) for preplant burndown weed control using a tank mix of Roundup PowerMax, 2,4-D LV4, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 (EPA Reg. No. 1381-102) (2-ethylhexyl ester of 2,4-dichlorophenoxyacetic acid 66.2%) was applied at 1 pt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 42 degrees F (5.6 degrees C). Relative Humidity was 50%. Wind was from north at 2 mph. Time of application was 10:30 a.m. - 2:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-04 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilzed microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer; the first pass was centered 15' from the west edge of the main plot, but half the boom (over the microplot area) was shut off. Conditions: Cloudy with air temperature 68 degrees F. Relative Humidity was 83%. Wind was calm, but out of the south at 0 mphs. Time of application was between 11:30 a.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 3.5 mph (gear B2, 1650 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11002 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2012-05-04 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilzed microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer; the first pass was centered 15' from the west edge of the main plot, but half the boom (over the microplot area) was shut off. Conditions: Cloudy with air temperature 68 degrees F. Relative Humidity was 83%. Wind was calm, but out of the south at 0 mphs. Time of application was between 11:30 a.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 3.5 mph (gear B2, 1650 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11002 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2012-05-04 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilzed microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer; the first pass was centered 15' from the west edge of the main plot, but half the boom (over the microplot area) was shut off. Conditions: Cloudy with air temperature 68 degrees F. Relative Humidity was 83%. Wind was calm, but out of the south at 0 mphs. Time of application was between 11:30 a.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 3.5 mph (gear B2, 1650 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11002 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2012-05-04 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatment G10, MICROPLOT area only, all replications (replications 1, 2, 3, 5 and 4). Treatment G10 microplots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs / gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas: fertilized microplot areas 15' wide located on the west side of treatment plots [G10R2, G10R3, G10R4, and G10R5] and located on the east side of the treatment plot G10R1; unfertilized areas which were the remaining 76.86' on the east side of the plots [G10R2, G10R3, G10R4, and G10R5] and located on the west side of treatment plot G10R1. The fertilized 15' microplot received 50 lbs N/A. The unfertilized 76.86' was NOT fertilized. Conditions: Cloudy with air temperature 68 degrees F. Relative Humidity was 83%. Wind was calm, but out of the south at 0 mphs. Time of application was between 11:30 a.m. and 4:15 p.m. Equipment Used: JD 5220 tractor traveling 3.5 mph (gear B2, 1650 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11002 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2012-05-10 Mechanical Weed Control Sprayed the LTER Main Site Treatment T2, all replications (reps 6, 5, 4, 3, 2 and 1) for preplant burndown weed control using Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 61 degrees F. Relative Humidity was 33%. Wind was from north at 2 mph. Time of application was 1:45 p.m. - 4:30 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-10 Fertilizer Application Fertilized GLBRC Main Site Switchgrass Fertility Study (SWF: N-rate), all treatments and all replications. Fertilizer applied as 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN) (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate).The amounts of nitrogen applied were as follows: SWF1 (108, 201, 307, 408) 0 lbs/A; SWF2 (104, 206, 308, 407) 25 lbs N/A (8.4 gal/A); SWF3 (102, 202, 302, 406) 50 lbs N/A (16.7 gal/A); SWF4 (105, 208, 305, 405) 75 lbs N/A (25.1 gal/A); SWF5 (101, 205, 304, 404) 100 lbs N/A (33.5 gal/A); SWF6 (107, 207, 301, 403) 125 lbs N/A (41.8 gal/A); SWF7 (106, 204, 306, 402) 150 lbs N/A (50.2 gal/A); SWF8 (103, 203, 303, 401) 175 lbs N/A (58.6 gal/A). In most cases, passes were made from the alleway area into the research plots: for blocks 1 and 2 passes were South to North; for blocks 3 and 4 passes were North to South. A total of 52 gallons of fertilizer were used which included for priming the spray boom and test applications into the trap crop area. Time of application was between 9:00 - 11:00 a.m. Temperature at start was approximately 46 degree F, 70% relative humidity, skies were clear and sunny. Wind was 5 mph out of northwest, with a 10 minute average of 4 mph. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. The sprayer was controlled by a Raven 440 automatic carrier control unit. Nozzles were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer; 12 nozzles were used on the boom. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 15-20 psi. No passes were made through the SWF1 treatment area, nor was any liquid (even water) applied. TeeJet 8001 nozzles were used for the SWF2 level. TurboTeeJet 11002 nozzles were used for the SWF3. TurboTeeJet 11003 nozzles were used for the SWF4, SWF5, and SWF6 levels. TurboTeeJet 11005 nozzles were used for the SWF7 and SWF8 levels.

2012-05-11 Mechanical Weed Control Sprayed the LTER Main Site Cellulosic Ethanol Study, treatments CE1 (plots 108, 202, 304, 401), CE2 (plots 112, 210, 312, 402), and CE3 (plots 105, 207, 301, 403) for preplant burndown weed control using Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 56 degrees F. Relative Humidity was 52%. Wind was from calm and from north at 0-2 mph. Time of application was 12:45 p.m. - 1:30 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-11 Mechanical Weed Control Sprayed the Interaction Study, no till treatments only for preplant burndown weed control using Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 56 degrees F. Relative Humidity was 52%. Wind was from calm and from north at 0-2 mph. Time of application was 10:00 - noon. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-11 Mechanical Weed Control Sprayed the LTER Main Site Treatments T8nt (no till component of treatment T8) all replications, for preplant burndown weed control using Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 56 degrees F. Relative Humidity was 52%. Wind was from calm and from north at 0-2 mph. Time of application was 10:00 - noon. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-11 Mechanical Weed Control Sprayed the ancillary plots (replicate 6) at the GLBRC main site preplant burndown weed control using Roundup PowerMax and ammonium sulfate. The ancillary plots which were sprayed were the 2nd-6th plots from the southwest corner of the main site area. Additionally, sprayed the easternmost pass of the guard bait crop on the east side of the field. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 56 degrees F. Relative Humidity was 52%. Wind was from calm and from north at 0-2 mph. Time of application was 10:00 - noon. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-14 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G1, all replications (reps. 1, 2, 3, 4 and 5) for pre-emergence weed control using Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] applied at 3 qts/acre (7.01 L/Ha). Weather conditions: Sunny with air temperature of 68 degrees F (20 degrees C). Relative Humidity was 96%. Wind was from calm and from the northwest at 0 mph (10 minute average). Time of application was 11:00 a.m. - 1:30 p.m. Equipment Used: JD 5220 tractor traveling 3.9 mph (gear B3, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-14 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G2, all replications (reps. 1, 2, 3, 4 and 5) for pre-emergence weed control using Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] applied at 3 qts/acre (7.01 L/Ha). Weather conditions: Sunny with air temperature of 68 degrees F (20 degrees C). Relative Humidity was 96%. Wind was from calm and from the northwest at 0 mph (10 minute average). Time of application was 11:00 a.m. - 1:30 p.m. Equipment Used: JD 5220 tractor traveling 3.9 mph (gear B3, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-14 Mechanical Weed Control Sprayed the LTER Main Site Treatment Cellulosic Ethanol Study, treatments CES1 and CES2, all replications [CES1 (plots 108, 202, 304, 401), CES2 (plots 112, 210, 312, 402)] for pre-emergence weed control in corn using Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] applied at 3 qts/acre (7.01 L/Ha). Weather conditions: Sunny with air temperature of 68 degrees F (20 degrees C). Relative Humidity was 96%. Wind was from calm and from the northwest at 0 mph (10 minute average). Time of application was 11:00 a.m. - 1:30 p.m. Equipment Used: JD 5220 tractor traveling 3.9 mph (gear B3, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-14 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G2, all replications (reps. 1, 2, 3, 4 and 5) for pre-emergence weed control using Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] applied at 3 qts/acre (7.01 L/Ha) and Ignite (Glufosinate-ammonium 24.5%: CAS # 77182-82-2) [EPA Reg. 264-829] at 29 ounces/A (0.35 L/HA). Weather conditions: Sunny with air temperature of 68 degrees F (20 degrees C). Relative Humidity was 96%. Wind was from calm and from the northwest at 0 mph (10 minute average). Time of application was 1:30 -3:00 p.m. Equipment Used: JD 5220 tractor traveling 3.9 mph (gear B3, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-14 Mechanical Weed Control Sprayed the GLBRC bait crop area, corn planting only which was the 30' closest to the GLBRC plots on the south, east and north side, for pre-emergence weed control using Lexar (S-metolachlor (+ safener) + atrazine + mesotrione) [EPA Reg. No. 100-1201] applied at 3 qts/acre (7.01 L/Ha) and Ignite (Glufosinate-ammonium 24.5%: CAS # 77182-82-2) [EPA Reg. 264-829] at 29 ounces/A (0.35 L/HA). Weather conditions: Sunny with air temperature of 68 degrees F (20 degrees C). Relative Humidity was 96%. Wind was from calm and from the northwest at 0 mph (10 minute average). Time of application was 1:30 -3:00 p.m. Equipment Used: JD 5220 tractor traveling 3.9 mph (gear B3, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-23 Mechanical Weed Control Sprayed the Lysimeter field for burndown weed control using Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 69 degrees F. Relative Humidity was 68%. Wind was from the southeast at 5 mph. Time of application was 12:00 p.m. - 2:30 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-23 Mechanical Weed Control Sprayed the ECB (European Corn Borer) field area for burndown weed control using Roundup PowerMax, 2,4-D LV4, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 (EPA Reg. No. 1381-102) (2-ethylhexyl ester of 2,4-dichlorophenoxyacetic acid 66.2%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. 2,4-D LV4 was only applied to the last 90' on the east side of the field area adn was not applied on the rest. Weather conditions: Sunny with air temperature of 69 degrees F. Relative Humidity was 68%. Wind was from the southeast at 5 mph. Time of application was 12:00 p.m. - 2:30 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-23 Herbicide Application Sprayed the LTER Main Site Treatement T7 tilled microplot area only, all replications (reps 6, 5, 1, 3, 4, and 2) for weed control using Roundup PowerMax, 2,4-D LV4, and ammonium sulfate. Principal weeds which were to be controlled were dogbane. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 (EPA Reg. No. 1381-102) (2-ethylhexyl ester of 2,4-dichlorophenoxyacetic acid 66.2%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Sprayed in two passes (30' each) going south to north finished by a headland pass (10' wide) across north side. Weather conditions: Sunny with air temperature of 74 degrees F. Relative Humidity was 48%. Wind was from the southeast at 5 mph. Time of application was 12:00 p.m. - 2:30 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-05-22 Mechanical Weed Control Sprayed the LTER Main Site Nitrogen rate study (N-Rate: Fertility Gradient, both irrigated and non-irrigated) field area for burndown weed control using Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Field was sprayed north-south with headland passes west-east on both the north and south sides. Weather conditions: mostly sunny with air temperature of 71 degrees F. Relative Humidity was 58%. Wind was from the northeast at 5 mph. Time of application was 5:15 p.m. - 7:00 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-06-08 Mechanical Weed Control Sprayed the Interaction field plot #3 only, weed control in soybeans. This plot was not sprayed when the rest the interaction field was sprayed in May. Sprayed using Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 78 degrees F. Relative Humidity was 48%. Wind was from the west at 1-8 mph. Time of application was 12:30 p.m. - 2:30 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-06-08 Mechanical Weed Control Sprayed the GLBRC bait crop areas not yet planted to corn for weed control using Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 78 degrees F. Relative Humidity was 48%. Wind was from the west at 1-8 mph. Time of application was 12:30 p.m. - 2:30 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2012-06-08 Fertilizer Application Fertilized the LTER Cellulosic Biofuels Experiment, treatments C4 Switchgrass (Cave-in-rock + N) (reps 110, 203, 302, and 404) and C6 Switchgrass (Southlow + N) (reps 111, 204, 310, and 408). All plots were fertilized with 50 lbs N/A applied as 28% N fertilizer (UAN) which is comprised of Urea + Ammonium Nitrate (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. All treatment plots were sprayed in one pass of the sprayer with the outside two nozzles shut off leaving the boom width, from outside to outside nozzles, of 27.5'. Conditions: Sunny with air temperature approximately 78 degrees F. Relative Humidity was 38%. Wind was from north at 3 mph. Time of application was between 11:15 and 11:59 a.m. Equipment Used: JD 5220 tractor traveling 2.7 mph (gear B3, 1450) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2012-06-25 Mechanical Weed Control Sprayed the LTER Main Site Treatment T2, all replications (reps 2, 3, 5, 4, 1 and 6) with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for post-emergence weed control in soybeans. Roundup Power Max was applied at 22 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 10 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier. Weather conditions at start were 61 degrees F (16.1 degrees C), 64% relative humidity, 10 minute wind velocity of 8 mph from NNE. Weather conditions at the end were 70 degrees F (21.1 degrees C), 39% relative humidity, 10 minute wind velocity of 12 mph from NNE. Because of the wind conditions, a lower boom pressure was used to create larger droplet size and reduce drift.

2012-06-26 Mechanical Weed Control Sprayed the LTER Main Site Treatment T8nt, all replications, Interaction Study area, GLBRC Productivity Study, and GLBRC replicate 6 area, soybeans only, with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for post-emergence weed control in soybeans. Roundup Power Max was applied at 22 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 10 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier. Weather conditions at 10 a.m. start were 68 degrees F (20.0 degrees C), 51% relative humidity, 10 minute wind velocity of 0 mph from W. Weather conditions at the 1 p.m. end were 75 degrees F (23.9 degrees C), 51% relative humidity, 10 minute wind velocity of 2 mph from W.

2012-06-22 Mechanical Weed Control Sprayed the LTER Main Site Treatment T1 and T3, all replications with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for post-emergence weed control in soybeans. Roundup Power Max was applied at 22 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 10 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier. Weather conditions at 9 a.m. start were 66 degrees F (18.9 degrees C), 43% relative humidity, 10 minute wind velocity of 0 mph from NW. Weather conditions at the 2:30 p.m. end were 79 degrees F (26.1 degrees C), 59% relative humidity, 10 minute wind velocity of 3 mph from NW. Treatment T3 info: Sprayed the LTER Main Site Treatment T3, all replications (reps 2, 3, 5, 4, 1 and 6) with Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl)glycine, in the form of its potassium salt, 48.7%) and Ammonium Sulfate (sprayable, CAS #7783-20-2) for post-emergence weed control in soybeans. Roundup Power Max was applied at 22 oz/A and the ammonium sulfate was applied at 3.4 lbs/A. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1400 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 10 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier. Weather conditions at start were 61 degrees F (16.1 degrees C), 64% relative humidity, 10 minute wind velocity of 8 mph from NNE. Weather conditions at the end were 70 degrees F (21.1 degrees C), 39% relative humidity, 10 minute wind velocity of 12 mph from NNE. Because of the wind conditions, a lower boom pressure was used to create larger droplet size and reduce drift.

2013-03-27 Planting Frost seeded LTER Main Site Treatment T4 with Medium Red Clover at 14 lbs/A. Clover seed was from DKB Farms & Services LLC, Columbiaville MI, 48421. Lot # WHIT-CLOV-12 : Certified Organic Clover Processor # 4023H1011-10. Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 20 inches apart. Gandy motor setting was 1.1 with the red metering wheels in and the gates on the Gandy were in the middle position. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Temperature was between 25-30 degrees F at the start and between 30-35 degrees F at the finish. All replicates were seeded in 20 passes across the field with the GPS set for a 3” overlap on each side during a pass. Replicate order was R5, R3, R4, R2, R6, R1. Due to the ground thawing quickly Rep 1 was seeded first thing on March 28, 2013.

2013-04-26 Mechanical Weed Control Sprayed LTER Main Site Lysimeter field for post emergence weed control in winter wheat. Plots sprayed with Affinity BroadSpec herbicide at .8 oz/A and Preference non-ionic surfactant at .25% v/v (1 qt/100 gal carrier). Time of application was 11:30am - 12:20pm. Weather conditions were sunny, temperature was 40°F at start and 54°F at end, 39% relative humidity, winds out of southeast at 6-12 mph with 10 minute average of 8 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2008-05-04 Herbicide Application Sprayed the LTER main site, treatment T6, replications 1-6, I believe this was a application to the winter wheat that was planted in the fall of 2007. Weather conditions: ? with air temperature of ? degrees F (?°C). Relative Humidity was ?%. Wind was from ? at ? mph. Time of application was 1:15-8:00 p.m. Equipment Used: JD 5220 tractor traveling ? mph (gear ?, ? rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet ? wide angle flat spray nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. ? psi were used. Water applied at ? gals/A, (? L/ha), was used as the carrier.

2008-05-05 Herbicide Application Sprayed the LTER T2 plots reps: 6, 1, 5, 2, 3, and 4 for weed burndown. Roundup Original Max 5.5L (gyphosate) was applied at ? quart/acre (? L/ha). Weather conditions: ? with air temperature of ? degrees F (? °C). Relative Humidity was ?%. Wind was ? mph. Time of application was ?. Equipment Used: JD 5220 tractor traveling ? mph (gear ?, ? rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet ? spray nozzles were used, spaced ? inches apart with a total boom length of 30 feet. ? psi were used. Water applied at ? gals/A, (? L/ha), was used as the carrier

2008-07-25 Herbicide Application Sprayed the LTER Main Site, Treatment 5 replications 1-6. Roundup (glyphosate) was applied at ? ounces/acre (? L/ha) with Ammonium Sulfate 17 lbs / 100 gallons (3.4 lbs/acre; 3.82 kg/ha). Weather conditions: ? with air temperature of ? degrees F (? °C). Relative Humidity was ?%. Wind was ?. Time of application was ? p.m. Equipment Used: JD 5220 tractor traveling ? mph (gear ?, ? rpm) with a TopAir sprayer with a ? pump. Turbo TeeJet ? nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. ? psi was used. Water applied at ? gallons/acre (? L/ha) was used as the carrier. Purpose of application: Stop regrowth of poplar trees, grasses and broadleaves.

2008-07-25 Herbicide Application Sprayed the LTER Main Site, Treatment 5 replications 1-6. Roundup (glyphosate) was applied at ? ounces/acre (? L/ha) with Ammonium Sulfate 17 lbs / 100 gallons (3.4 lbs/acre; 3.82 kg/ha). Weather conditions: ? with air temperature of ? degrees F (? °C). Relative Humidity was ?%. Wind was ?. Time of application was ? p.m. Equipment Used: JD 5220 tractor traveling ? mph (gear ?, ? rpm) with a TopAir sprayer with a ? pump. Turbo TeeJet ? nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. ? psi was used. Water applied at ? gallons/acre (? L/ha) was used as the carrier. Purpose of application: Stop regrowth of poplar trees, grasses and broadleaves.

2013-04-29 Herbicide Application Sprayed LTER Main Site Treatment T1 all replications (5, 1, 4, 2, 3, 6), for post emergence weed control in winter wheat. Rep 6 in the last half a swath on the west side of the plot the pump quit working on the sprayer. That section was sprayed on May 1, 2013. Plots sprayed with a tank mix of Anti-Foaming agent at 1oz/100 gal of carrier, Ammonium Sulfate at 2lbs/A, Osprey Herbicide at 4.75oz/A, Affinity BroadSpec herbicide at 0.8 oz/A, and Preference non-ionic surfactant at 0.25% v/v (1 qt/100 gal carrier). Time of application was 12:30 - 2:30 p.m. Weather conditions were sunny, temperature was 64°F at start and 72°F at end, winds out of the southwest at 2-10 mph with 10 minute average of 4 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Nozzles were Turbo Teejet 11003 flat fans spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-03-28 Planting Frost seeded clover into 2013 Biodiversity wheat plots with cover crop. Organic medium red clover seed was frost seeded into Biodiversity treatments B4 (104, 220, 307, 404), B7 (105, 219, 305, 407), B17 (116, 211, 302, 417), all of which had winter wheat planted in November 2012. Clover was seeded at 14 lbs/A in two passes of a Gandy air seeder with a 15' boom length. Weather conditions at time of seeding were between 30-35 degrees F and partly sunny.

2013-03-28 Planting Frost seeded LTER Main Site Treatment T3 with Medium Red Clover at 14 lbs/A. Clover seed was from DKB Farms & Services LLC, Columbiaville MI, 48421. Lot # WHIT-CLOV-12 : Certified Organic Clover Processor # 4023H1011-10. Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 20 inches apart. Gandy motor setting was 1.1 with the red metering wheels in and the gates on the Gandy were in the middle position. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Temperature was between 25-30 degrees F at the start and between 30-35 degrees F at the finish. All replicates were seeded in 20 passes across the field with the GPS set for a 3” overlap on each side during a pass. Replicate order was R6, R1, R2, R3, R4, R5.

2013-05-01 Mechanical Weed Control Sprayed LTER Main Site T3 plots all replications (6, 1, 3, 2, 4, 5), for post emergence weed control in winter wheat. Plots sprayed with Affinity BroadSpec herbicide at 1 oz/A and Preference non-ionic surfactant at .25% v/v (1 qt/100 gal carrier). Time of application was 3:00 - 5:15 pm. Weather conditions were sunny, temperature was 81 Degrees ferinheight, 37% relative humidity, winds out of southeast at 2-10 mph with 10 minute average of 5 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-05-03 Fertilizer Application Fertilized LTER Main Site Treatment 3 Fertilized Micro-plots, all replications (reps 1, 2, 3, 4, 5, and 6). Microplot area is located in the northwest corner of each replication(50 feet deep [north to south] and 90 feet long [east to west]) and is divided into 6 microplots, each one measures 15' (e-w) x 50' (n-s). Fertilized microplots were the following: T3R1 (1, 3, 4), T3R2 (1, 3, 4), T3R3 (1, 3, 5), T3R4 (3, 4, 6), T3R5 (1, 2, 4), and T3R6 (2, 3, 4). Applied 16 gal/A 28% nitrogen solution (28-0-0) in the microplots; one gallon of 28% fertilizer weighs 10.67 lbs. so 48 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 76 degrees F. Relative Humidity was 54%. Wind was out of the ESE at 5-10 mphs at start. Time of application was between 11:30 a.m. and 1:00 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 15 feet (right side of boom only). 30 psi were used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2013-05-03 Fertilizer Application Fertilized LTER Main Site Treatment 4 Fertilized Micro-plots, all replications (reps 6, 1, 2, 3, 4 and 5). Microplot area is located in the northwest corner of each replication(50 feet deep [north to south] and 90 feet long [east to west]) and is divided into 6 microplots, each one measures 15' (e-w) x 50' (n-s). Fertilized microplots were the following: T4R1 (1, 3, 4), T4R2 (1, 4, 6), T4R3 (2, 4, 5), T4R4 (1, 3, 6), T4R5 (2, 4, 6), and T4R6 (2, 3, 4). Applied 27 gal/A 28% nitrogen solution (28-0-0) in the microplots; one gallon of 28% fertilizer weighs 10.67 lbs. so 80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 76 degrees F. Relative Humidity was 54%. Wind was out of the ESE at 5-10 mphs at start. Time of application was between 11:30 a.m. and 1:00 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 15 feet (right side of boom only). 30 psi were used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2013-05-03 Fertilizer Application Fertilized LTER Main Site Treatment T3 all replications (reps. 1, 2, 3, 4, 5 and 6). Applied 28% nitrogen solution (28-0-0) at 16 gals/acre. Unfertilized microplots which were located in the northwest corner (50 feet deep [north to south] and 90 feet long [east to west]) were not fertilized. Total amount of fertilizer applied was 224 gallons on 14.0 acres. One gallon of 28% fertilizer weighs 10.67 lbs. so 48 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 79 degrees F. Relative Humidity was 44%. Wind was out of the south by southeast at 5-10 mphs with 10 minute average speed of 4 mphs at start. Time of application was between 3:30 a.m. and 5:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 10-20 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2013-05-02 Fertilizer Application Fertilized LTER Main Site Treatment T1 all replications (reps.6, 3, 4, 1, 5, 2). Applied 28% nitrogen solution (28-0-0) at 27 gals/acre. Unfertilized micro-plots which were located in the northwest corner (5 meters deep [north to south] and 40 meters long [east to west]) were not fertilized. Total amount of fertilizer applied was 386 gallons on 14.3 acres. One gallon of 28% fertilizer weighs 10.67 lbs. 80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 82 degrees F. Wind was out of the southwest at 2-10 mph averaging 5 mph at start. Time of application was between 1:30 p.m. and 3:45 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11005 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 10-20 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2013-05-02 Fertilizer Application Fertilized LTER Main Site Treatment T2 all replications (reps.1, 2, 3, 4, 5, 6). Applied 28% nitrogen solution (28-0-0) at 27 gals/acre. Unfertilized micro-plots which were located in the northwest corner (5 meters deep [north to south] and 40 meters long [east to west]) were not fertilized. Total amount of fertilizer applied was 386 gallons on 14.3 acres. One gallon of 28% fertilizer weighs 10.67 lbs. 80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 82 degrees F. Wind was out of the southwest at 2-10 mph averaging 5 mph at start. Time of application was between 3:45 p.m. and 5:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11005 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 10-20 psi was used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2013-05-02 Mechanical Weed Control Sprayed LTER T3 Micro-plots BAU for post emergence weed control in winter wheat. Plots sprayed with Affinity BroadSpec herbicide at 1 oz/A and Preference non-ionic surfactant at .25% v/v (1 qt/100 gal carrier). Time of application was 9:45 am to 12:00 pm. Weather conditions were sunny, temperature was 70 Degrees ferinheight, winds out of south at 1-7 mph with 10 minute average of 4 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-05-02 Mechanical Weed Control Sprayed Interaction plots for post emergence weed control in winter wheat. East 15 ft of every plot were not sprayed. Plots sprayed with Affinity BroadSpec herbicide at 1 oz/A and Preference non-ionic surfactant at .25% v/v (1 qt/100 gal carrier). Time of application was 9:45 am to 12:00 pm. Weather conditions were sunny, temperature was 70 Degrees ferinheight, winds out of south at 1-7 mph with 10 minute average of 4 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-05-02 Mechanical Weed Control Sprayed LTER T8NT plots in order of (20, 24, 17, 21), for post emergence weed control in winter wheat. Plots sprayed with Affinity BroadSpec herbicide at 1 oz/A and Preference non-ionic surfactant at .25% v/v (1 qt/100 gal carrier). Time of application was 9:45 am to 12:00 pm. Weather conditions were sunny, temperature was 70 Degrees ferinheight, winds out of south at 1-7 mph with 10 minute average of 4 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-05-03 Fertilizer Application Fertilized LTER Main Site Lysimeter Field with 28% nitrogen. Applied 28% nitrogen solution (28-0-0) across most of field at 27 gals/acre (2 swaths left on east side of field). One gallon of 28% fertilizer weighs 10.67 lbs. so 80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 79 degrees F. Relative Humidity was 44%. Wind was out of the SE at 5-10 mphs at start. Time of application was between 5:00 p.m. and 5:30 p.m. Equipment Used: JD 5220 tractor traveling 4.6 mph (gear C1, 1450 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11005 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen fertilizer for the winter wheat.

2013-05-04 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G1, all replications (reps. 5, 4, 2, 3, 1) for preplant burndown weed control using a tank mix of Roundup PowerMax, and ammonium sulfate. Roundup Power Max (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate was applied at 17 lbs/100 gal water carrier. Weather conditions: Sunny with air temperature of 64 degrees F. Relative Humidity was 45%. Wind was from south at 6-10 mph. Time of application was 9:30 a.m. - 12:00 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2013-05-04 Mechanical Weed Control Sprayed the GLBRC bait crop areas not yet planted for weed control using Roundup PowerMax and ammonium sulfate. Roundup Power Max (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 64 degrees F. Relative Humidity was 45%. Wind was from the south at 5-10 mph. Time of application was 9:30 a.m. - 12:00 p.m. North and South areas are sprayed, East edge the middle strip is sprayed and half of the west strip. Strip next to the woods has not been sprayed.Equipment Used: JD 5220 tractor traveling 5 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2013-05-07 Mechanical Weed Control Sprayed the GLBRC bait crop areas not yet planted for weed control using Roundup PowerMax, 2, 4-D LV-4 and ammonium sulfate. Roundup Power Max (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2, 4-D LV-4 applied at 1 pt/A and Ammonium Sulfate was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 73 degrees F. Relative Humidity was 47%. Wind was from the south at 1-8 mph. Time of application was 2:30 p.m. - 3:30 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2013-05-07 Mechanical Weed Control Sprayed the LTER Main Site Cellulosic Ethanol Study, treatments CE1 (plots 108, 202, 304, 401), CE2 (plots 112, 210, 312, 402), and CE3 (plots 105, 207, 301, 403) for preplant burndown weed control using Roundup PowerMax, 2,4D-LV-4 and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 was applied at 1 pt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: Sunny with air temperature of 73 degrees F. Relative Humidity was 41%. Wind was from ENE 1-8 mph with 10 min avg. of 5 mph. Time of application was 1:15 p.m. - 2:30 p.m. Equipment Used: JD 5220 tractor traveling 3.8 mph (gear B3, 1700 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2013-05-08 Fertilizer Application Fertilized LTER N-rate study (Irrigated and Non-irrigated fertility gradient study) winter wheat plots. Fertilizer applications were with 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN). A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. The amounts of nitrogen applied were as follows: F1 (101, 203, 309, 404, 501, 603, 709, 804) 0 lbs/A; F2 (102, 202, 304, 406, 502, 602, 704, 806) 20 lbs N/A (7 gal/A); F3 (103, 205, 302, 407, 503, 605, 702, 807) 40 lbs N/A (13.5 gal/A); F4 (104, 208, 305, 403, 504, 608, 705, 803) 60 lbs N/A (20 gal/A); F5 (105, 204, 306, 401, 505, 604, 706, 801) 80 lbs N/A (27 gal/A); F6 (106, 209, 308, 402, 506, 609, 708, 802) 100 lbs N/A (33.5 gal/A); F7 (107, 201, 307, 408, 507, 601, 707, 808) 120 lbs N/A (40 gal/A); F8 (108, 207, 303, 405, 508, 607, 703, 805) 140 lbs N/A (47 gal/A); F9 (109, 206, 301, 409, 509, 606, 701, 809) 160 lbs N/A (53.5 gal/A). Guard plots were sprayed at rates comparable to adjacent treatment plots; i.e. west of 101, unsprayed; west of 301 sprayed at F9 rate; east of 609 sprayed at F6 rate; east of 809 sprayed at F9 rate; middle buffer plots between replications 4 & 7 were sprayed at 160 lbs N/A. Time of application was between 10:00 a.m. and 3:30 p.m. Temperature was 74F, 47% relative humidity, skies were mostly sunny. Wind was between 1-5 mph at start NE. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. The sprayer was controlled by a John Deere Rate Controller. Nozzles were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 20-35 psi. TeeJet 8001 nozzles were used for the F2 level. TurboTeeJet 11002 nozzles were used for the F3 and F4 levels. TurboTeeJet 11005 nozzles were used for the F5, F6, F7, F8 and F9 levels.

2013-05-09 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G1, all replications (reps. 5, 4, 3, 2, 1) for pre-emerge weed control using Lexar at 3qts/A. Weather conditions: Sunny with air temperature of 76 degrees F. Relative Humidity was 34%. Wind was from WNW at 6-8mph. Time of application was 1:30 p.m. - 2:15 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2013-05-09 Mechanical Weed Control Sprayed bait crop for pre-emerge weed control where corn was planted. Corn was planted on the north edge of the north side of the GLBRC and then planted in the middle 30' on the east and south side of the site. Sprayed Lexar at a rate of 3 qts/A. Weather conditions were sunny 76 degrees F, 34% Humidity with 6mph wind out of WNW.Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2013-05-16 Fertilizer Application Fertilize winter wheat in LTER T8-NT plots (17, 21, 24, 20) with 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN) (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). Applied 27 gallons per acre which is 81 lbs of N per acre. Plots were completed in two passes using 300 gal top air sprayer controlled by John Deere Rate controller and pulled by John Deere 5220 tractor. Time of Application was 3:00- 4:00 p.m., weather was 80 degrees F 28% Humidity and winds were 1-8 mph out of the WNW.

2013-05-16 Fertilizer Application Fertilized Interaction plots (16, 15, 14, 10, 12, 7, 1, 5) with 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN) (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). Purpose of fertilization is to supply N to winter wheat. A rate of 27 gallons per acre of 28% was applied which is equivalent to 81 lbs of N/A. Plots were completed in 3 passes using 300 gallon top air sprayer with a 30' boom width controlled by John Deere Rate controller all pulled by John Deere 5220 tractor. Time of application was 3:00 - 4:00 p.m. Weather conditions were sunny 80 degrees F, 28% Humidity and winds 1-8 mph out of the WNW.

2013-05-16 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatment G10, MICROPLOT area only, all replications (replications 5, 4, 3, 2, and 1). Treatment G10 microplots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs / gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas: fertilized microplot areas 15' wide located on the west side of treatment plots [G10R2, G10R3, G10R4, and G10R5] and located on the east side of the treatment plot G10R1; unfertilized areas which were the remaining 76.86' on the east side of the plots [G10R2, G10R3, G10R4, and G10R5] and located on the west side of treatment plot G10R1. The fertilized 15' microplot received 50 lbs N/A. The unfertilized 76.86' was NOT fertilized. Conditions: Sunny 70 degrees F 42% Humidity and winds 1-5 mph NW. Equipment Used: JD 5220 tractor traveling 3.5 mph (gear B2, 1650 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart. Purpose of Application: Supply nitrogen to the treatment plots.

2013-05-16 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Each treatment plot was divided into two areas, an unfertilzed microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. Conditions: Sunny, 80 degrees F, 28% Humidity with winds 1-8 mph NW. Time of application 1:00 - 3:00 p.m. Equipment Used: JD 5220 tractor traveling 3.5 mph (gear B2, 1650 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2013-05-16 Fertilizer Application Fertilized GLBRC Main Site Switchgrass Fertility Study (SWF: N-rate), all treatments and all replications. Fertilizer applied as 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN) (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate).The amounts of nitrogen applied were as follows: SWF1 (108, 201, 307, 408) 0 lbs/A; SWF2 (104, 206, 308, 407) 25 lbs N/A (8.4 gal/A); SWF3 (102, 202, 302, 406) 50 lbs N/A (16.7 gal/A); SWF4 (105, 208, 305, 405) 75 lbs N/A (25.1 gal/A); SWF5 (101, 205, 304, 404) 100 lbs N/A (33.5 gal/A); SWF6 (107, 207, 301, 403) 125 lbs N/A (41.8 gal/A); SWF7 (106, 204, 306, 402) 150 lbs N/A (50.2 gal/A); SWF8 (103, 203, 303, 401) 175 lbs N/A (58.6 gal/A). All passes except for F2 & F3 applications were made from the south alleyway across to the north side, doing blocks 1 & 3 at a time and then 2 & 4. Time of application was between 9:30 - 11:30 a.m. Temperature at start was approximately 70 degree F, 42% relative humidity, skies were clear and sunny. Wind was 1-6 mph out of northwest. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. The sprayer was controlled by a John Deere Rate Controller. Nozzles were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer; 11 nozzles were used on the boom. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 15-20 psi. TeeJet 8001 nozzles were used for the SWF2 level. TurboTeeJet 11002 nozzles were used for the SWF3. TurboTeeJet 11003 nozzles were used for the SWF4, SWF5, levels. TurboTeeJet 11005 nozzles were used for the SWF7, SWF7 and SWF8 levels.

2013-05-17 Mechanical Weed Control Sprayed (Replication 1) only of the GLBRC switchgrass variety study to control broad leaf weeds. Applied 2,4-D LV-4 at a rate of 1qt/A on all plots. Equipment used was John Deere 5220 tractor pulling a Top Air 300 gallon sprayer with 30' overall boom length controlled by a Green Star Rate Controller. Tee-Jet 11003 nozzles were used spaced 15 inches apart. Started at 10:30 a.m. and ended at 11:30 a.m. Weather conditions were mostly sunny 63 degrees F with 46% Humidity and winds 1-8 mph with a 10 minute average of 4 mph.

2013-05-17 Mechanical Weed Control Spot sprayed LTER Main Site T2's All Replications to control blue grass. Applied AMS at 3.0 lbs/A, Osprey at 4.75 oz/A and Non Ionic Surfactant at .5 % v/v. Sections of the booms were turned on and off as needed while traveling across all plots. Below is a brief description of each replication. Rep 1 the south half was sprayed all the way across and then a full boom width across the north and east edge as well was sprayed. Rep 2 the micro-plot to the west was sprayed and then a full 2 booms widths were sprayed around the remaining border edges around the plot. Rep 3 half way into the micro-plot and to the west was sprayed and then a full 2 booms widths were sprayed on the remaining border edges around the plot. Rep 4 a full boom width was sprayed around the border edges of the plot. Sections were turned on and off as needed. Rep 5 from micro-plot to the west edge of the plot was sprayed completely. While making pass across the plot to the east the sprayer was turned on and off as needed to apply coverage across the hill that runs through the plot. Remaining border edges were sprayed with 2 booms as well. Rep 6 a full boom width was sprayed around the border edges of the plot. Sections were turned on and off as needed. Started at 1:15 p.m. weather conditions were partly cloudy 70 degrees F, 42 % Humidity with wind 1-5 mph out the the EN.

2013-05-17 Mechanical Weed Control Sprayed GLBRC switchgrass fertility study to control broad leaf weeds. Applied 2,4-D LV-4 at a rate of 1qt/A on all plots. Equipment used was John Deere 5220 tractor pulling a Top Air 300 gallon sprayer with 30' overall boom length controlled by a Green Star Rate Controller. Tee-Jet 11003 nozzles were used spaced 15 inches apart. Started at 10:30 a.m. and ended at 11:30 a.m. Weather conditions were mostly sunny 63 degrees F with 46% Humidity and winds 1-8 mph with a 10 minute average of 4 mph.

2013-06-14 Mechanical Weed Control Sprayed the GLBRC Main Site area rep 6 (5 plots east of miscanthus plot in rep 6) and the east side of the bait crop where soybeans and corn have already been planted for post emergence weed control using Roundup Powermax and ammonium sulfate. Roundup Powermax was applied at 1 qt/A and ammonium sulfate was applied at 17 lbs/100 gallons carrier (water). Start time of application 2:00 p.m. Weather conditions were mostly sunny, 73 degrees F, 53% humidity with 5 mph NE wind. End of application was 5:15 weather was mostly sunny, 77 degrees F, 36% humidity with 1-5 mph N winds.

2013-06-14 Mechanical Weed Control Sprayed GLBRC Main Site area next to brown house and outer edge of bait crop on the east and south edge for pre-plant burn down using Roundup Powermax and ammonium sulfate. Roundup Powermax was applied at 1 qt/A and ammonium sulfate was applied at 17 lbs/100 gallons carrier (water). Start time of application 2:00 p.m. Weather conditions were mostly sunny, 73 degrees F, 53% humidity with 5 mph NE wind. End of application was 5:15 weather was mostly sunny, 77 degrees F, 36% humidity with 1-5 mph N winds.

2013-06-14 Mechanical Weed Control Sprayed the GLBRC Main Site Treatment G1, G2, G3, G4 all replications (reps. 5,4,3,2,1) for post emergence weed control using Roundup Powermax and ammonium sulfate. Roundup Powermax was applied at 1 qt/A and ammonium sulfate was applied at 17 lbs/100 gallons carrier (water). Start time of application 2:00 p.m. Weather conditions were mostly sunny, 73 degrees F, 53% humidity with 5 mph NE wind. End of application was 5:15 weather was mostly sunny, 77 degrees F, 36% humidity with 1-5 mph N winds.

2013-06-14 Mechanical Weed Control Sprayed the LTER Main Site Cellulosic Ethanol Study, treatments CE1 (plots 108, 202, 304, 401), CE2 (plots 112, 210, 312, 402), and CE3 (plots 105, 207, 301, 403) for post emergence weed control using Roundup PowerMax and ammonium sulfate. Roundup Powermax was applied at 1 qt/A and Ammonium Sulfate was applied at 17 lbs / 100 gallons of carrier (water). Start time of application was 1:30 p.m. and weather conditions were 73 degrees F mostly sunny, 53% humidity with 5 mph NE wind with 10 min average of 3 mph. Sprayed 20 gal/A using water as the carrier and the last end nozzle on each side of the boom was turned off.

2013-07-15 Planting Seeded Biodiversity Study treatments B5, B8, and B15, with red clover into field corn. The treatments which were seeded were corn plots which were suppose to have leguminous cover crops as part of their rotation: B5 (plots 114, 217, 312, 405), B8 (plots 118, 214, 310, 408), B15 (plots 112, 213, 301, 415). Clover was seeded at 12 lbs/A using the small 15' Gandy with band applicators set up for 30 in rows (7 bands in total). Corn was around 18-30 inches tall at time of application.

2013-08-10 Mechanical Weed Control Sprayed LTER Main Site T3 plots all replication for post harvest / pre-plant burn down. All replications sprayed in this order (1, 2, 3, 4, 5, and 6) with RoundUp Power Max at 32 oz/A, Ammonium Sulfate at 17 lbs/ 100 gal of carrier, and anti-foaming agent at 1 oz/ 100 gal of carrier. Time of application was 12:30 p.m. with weather conditions mostly sunny 73 degrees F, 88% humidity with winds 3-5 mph NWN. End time of application was 2:40 with weather conditions mostly sunny 75 degrees F, 49% humidity with winds 5-8 mph NW.

2013-08-03 Mechanical Weed Control Sprayed LTER CE Study C2 Soybeans all replications in this order (3, 1, 2, 4) with RoundUp Power Max at 32 oz/A, Ammonium Sulfate at 17 lbs/ 100 gal of carrier, and anti-foaming agent at 1 oz/ 100 gal of carrier. Time of application was 2:20 p.m. to 2:40 p.m. with weather conditions mostly sunny 75 degrees F, 49% humidity with winds 5-8 mph NW.

2013-08-26 Mechanical Weed Control Sprayed LTER Main Site Fertility Gradient (N-rate) Study, including irrigated and dry-land areas, all replications for post wheat harvest weed control. Plots sprayed with Roundup PowerMax herbicide at 1 qt/A, 17 lbs / 100 gallons/carrier of ammonium sulfate and 1 oz / 100 gallons/carrier of anti foaming agent. Time of application was from 9:30 a.m. to 12:15 p.m. Weather conditions at the beginning were mostly cloudy with some sun 74 degrees F, 70 % humidity and winds 2-5 mph sw, weather conditions at the end were mostly cloudy with some sun 79 degrees F, 64% humidity and winds 3-8 mph sw. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-08-26 Mechanical Weed Control Sprayed LTER Main Site Lysimeter field for post wheat harvest weed control. Plots sprayed with Roundup PowerMax herbicide at 1 qt/A, 17 lbs / 100 gallons of carrier of ammonium sulfate and 1 oz / 100 gallons of carrier of anti foaming agent. Time of application was from 9:30 a.m. to 12:15 p.m. Weather conditions at the beginning were mostly cloudy with some sun 74 degrees F, 70 % humidity and winds 2-5 mph sw, weather conditions at the end were mostly cloudy with some sun 79 degrees F, 64% humidity and winds 3-8 mph sw. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-08-28 Mechanical Weed Control Sprayed LTER Interaction plots for post wheat harvest weed control. Plots sprayed with Roundup PowerMax herbicide at 1 qt/A, 17 lbs / 100 gallons/carrier of ammonium sulfate and 1 oz / 100 gallons/carrier of anti foaming agent. Time of application was from 1:50 p.m. - 3:15 p.m. Weather conditions at the beginning were cloudy 76 degrees F, 84 % humidity and winds 3 mph N, weather conditions at the end were cloudy 77 degrees F, 82 % humidity and winds 3-6 mph N. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-08-29 Herbicide Application Sprayed LTER Main Site T1 plots all replications (1, 5, 2, 3, 4, and 6) for post wheat harvest weed control. T1 R1 the entire micro-plot was accidentally sprayed while doing the head land swath. Plots sprayed with Roundup PowerMax herbicide at 1 qt/A, 17 lbs / 100 gallons/carrier of ammonium sulfate and 1 oz / 100 gallons/carrier of anti foaming agent. Time of application was from 11:15 a.m. - 1:40 p.m. Weather conditions at the beginning were sunny 77 degrees F, 73 % humidity and winds 2-3 mph E, weather conditions at the end were sunny 81 degrees F, 71 % humidity and winds calm out of the E. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2013-08-29 Mechanical Weed Control Sprayed LTER Main Site T2 plots all replications (1, 2, 3, 4, 5, and 6) for post wheat harvest weed control. Plots sprayed with Roundup PowerMax herbicide at 1 qt/A, 17 lbs / 100 gallons/carrier of ammonium sulfate and 1 oz / 100 gallons/carrier of anti foaming agent. Time of application was from 2:30 p.m. - 5:15 p.m. Weather conditions at the beginning were sunny 81 degrees F, 71 % humidity and winds calm out of the E, weather conditions at the end were sunny 84 degrees F, 71 % humidity and winds calm out of the E. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A (187 L/Ha) was used as the carrier.

2006-11-10 Planting Planted winter wheat, Pioneer 25R37 untreated seed, on the main site treatment 4, all replications. Planted with the JD 5220 tractor and JD 450 conventional 10' drill. Planting depth was 1 inch.

2006-11-09 Planting Planted winter wheat, Pioneer 25R37 treated seed, on the main site treatment 3, all replications. Planted with the JD 5220 tractor and JD 450 conventional 10' drill. Planting depth was 1 inch.

2006-07-07 Herbicide Application Sprayed all reps of T2 plots. I do have a spray report from this application. I assume that we would have applied some of the same herbicides applied to T3 plots on July 7, 2006.

2006-07-07 Herbicide Application Sprayed all reps of T1 plots. I am not sure what date this application was made. I do have a spray report from this application. I assume that we would have applied some of the same herbicides applied to T3 plots on July 7, 2006.

2007-04-20 Herbicide Application Sprayed winter wheat in LTER T2 plots reps: 6, 1, 2, 3, 5, and 4 for control of broadleave weeds. Please see attached report for detailed application information.

2007-04-21 Herbicide Application Sprayed winter wheat in LTER T1 plots reps: 6, 3, 2, 4, 5, and 1 for control of broadleave weeds. Please see attached report for detailed application information.

2007-04-21 Herbicide Application Sprayed winter wheat in T8nt, N-rate (irrigated and non-irrigated), and lysimeter field for control of broadleave weeds. Please see attached report for detailed application information.

2007-04-24 Fertilizer Application Fertilized LTER Main Site Treatment T1 all replications (reps: 4, 3, 2, 1, 5, and 6). Applied 28% nitrogen solution (28-0-0) at 16 gals/acre. Purpose of Application: Supply nitrogen fertilizer for the winter wheat. Unfertilized micro-plots which were located in the northwest corner (5 meters deep [north to south] and 40 meters long [east to west]) were not fertilized. One gallon of 28% fertilizer weighs 10.67 lbs. 47.80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 63 degrees F. Wind was out of the north-northwest at 0-5 mph averaging 2 mph at start. Time of application was between 1:00 p.m. and 3:00 p.m. Equipment Used: JD 5220 tractor traveling 5.0-5.5 mph (gear C1, 1600-1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. Please see attached report for detailed application information.

2007-04-24 Fertilizer Application Fertilized LTER Main Site Treatment T2 all replications (reps: 6, 1, 5, 2, 3, and 4). Applied 28% nitrogen solution (28-0-0) at 16 gals/acre. Purpose of Application: Supply nitrogen fertilizer for the winter wheat. Unfertilized micro-plots which were located in the northwest corner (5 meters deep [north to south] and 40 meters long [east to west]) were not fertilized. One gallon of 28% fertilizer weighs 10.67 lbs. 47.80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 55 degrees F. Wind was out of the east-northeast at 0-7 mph averaging 4 mph at start. Time of application was between 10:00 a.m. and 11:45 a.m. Equipment Used: JD 5220 tractor traveling 5.0-5.5 mph (gear C1, 1600-1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. Please see attached report for detailed application information.

2007-04-24 Fertilizer Application Fertilized LTER Treatment T8nt all replications. Applied 28% nitrogen solution (28-0-0) at 16 gals/acre. Purpose of Application: Supply nitrogen fertilizer for the winter wheat. One gallon of 28% fertilizer weighs 10.67 lbs. 47.80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 66 degrees F. Wind was out of the north at 0-5 mph averaging 2 mph at start. Time of application 3:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0 mph (gear C1, 1600 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. Please see attached report for detailed application information.

2007-04-24 Fertilizer Application Fertilized LTER Lysimeter Field. Applied 28% nitrogen solution (28-0-0) at 16 gals/acre. Purpose of Application: Supply nitrogen fertilizer for the winter wheat. One gallon of 28% fertilizer weighs 10.67 lbs. 47.80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 66 degrees F. Wind was out of the north at 0-5 mph averaging 2 mph at start. Time of application 3:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0-5.5 mph (gear C1, 1600-1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. Please see attached report for detailed application information.

2007-04-30 Fertilizer Application Fertilized LTER Main Site T3 plots all replications (reps: 6, 1, 3, 2, 4, and 5). Applied 28% nitrogen solution (28-0-0) at 16 gals/acre. Purpose of Application: Supply nitrogen fertilizer for the winter wheat. One gallon of 28% fertilizer weighs 10.67 lbs. 47.80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 54 degrees F. Wind was out of the north-northeast at 0-7 mph averaging 4 mph at start. Time of application was between 8:00 a.m. and 10:00 a.m. Equipment Used: JD 5220 tractor traveling 5.0-5.5 mph (gear C1, 1600-1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. Please see attached report for detailed application information.

2007-04-30 Fertilizer Application Fertilized LTER T3 and T4 micro-plots. Applied 28% nitrogen solution (28-0-0) at 16 gals/acre. Purpose of Application: Supply nitrogen fertilizer for the winter wheat. One gallon of 28% fertilizer weighs 10.67 lbs. 47.80 lbs N/A was applied. Weather Conditions: Mostly sunny with air temperature 67 degrees F. Wind was out of the north at 0-12 mph averaging 9 mph at start. Time of application 1:30 p.m. Please see attached report for detailed application information.

2007-09-24 Herbicide Application Sprayed wheat stubble on LTER main site T2 plots, all replications, reps: 6, 1, 5, 2, 3, and 4. Purpose of Application: Control annual and perennial weeds. Weather Conditions: Mostly sunny with air temperature 71 degrees F. Wind was out of the south-southeast at 0-5 mph averaging 3 mph at start. Time of application was between 10:00 a.m. and 11:45 a.m. Equipment Used: JD 5220 tractor traveling 5.5-6.0 mph (gear C1, 1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. Please see attached report for detailed application information.

2007-09-29 Herbicide Application Sprayed alfalfa on LTER main site T6 plots, all replications, reps: 6, 5, 3, 4, 2, and 1. Purpose of Application: Remove alfalfa from plots. Weather Conditions: Mostly sunny with air temperature 74 degrees F. Wind was out of the south at 0-6 mph averaging 2 mph at start. Time of application was between 2:30 p.m. and 4:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0-6.0 mph (gear C1, 1600-1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. Please see attached report for detailed application information.

2007-09-29 Herbicide Application Sprayed wheat stubble on LTER T8nt plots, N-rate (irrigated and non-irrigated studies), and Lysimeter Field. Purpose of Application: Control annual and perennial weeds. Weather Conditions: Mostly sunny with air temperature 72 degrees F. Wind was out of the south-southeast at 0-4 mph averaging 2 mph at start. Time of application was between 5:30 p.m. and 7:30 p.m. Equipment Used: JD 5220 tractor traveling 5.0-6.0 mph (gear C1, 1600-1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. Please see attached report for detailed application information.

2007-05-04 Fertilizer Application Fertilized LTER N-rate studies (Irrigated and Non-irrigated). Applied 28% nitrogen solution (28-0-0) at 16 gals/acre. Purpose of Application: Supply nitrogen fertilizer for the winter wheat. One gallon of 28% fertilizer weighs 10.67 lbs. Please see attached report for details of application.

2007-04-21 Herbicide Application Sprayed winter wheat in Lysimeter Field. Purpose of application: control of annual broadleave weeds. Please see attached report for detailed application information.

2007-05-23 Herbicide Application Sprayed ECB today. I do not have the spray report for this application.

2007-07-30 Herbicide Application Sprayed the old N-rate study area (the area on the west side of the LTER main drive just inside the LTER main gate). The purpose of this application was to prepare this field area for planting of crops/treatments for the new LTER Cellulosic Ethanol Study (CE) in the spring of 2008. I do not have a spray report for this application.

2007-09-24 Herbicide Application Sprayed the old N-rate study area (the area on the west side of the LTER main drive just inside the LTER main gate). The purpose of this application was to prepare this field area for planting of crops/treatments for the new LTER Cellulosic Ethanol Study (CE) in the spring of 2008. I do not have a spray report for this application.

2004-04-30 Herbicide Application Sprayed the winter wheat on the main site lysimeter field for weeds. Purpose of Application: Control of annual broadleaves. Sprayed postemergence. 2,4-D Amine was applied at 1 pt/A, (1.17 L/ha). A total of 1 gallon of 2,4-D was applied on 8.3 acres of the field (3.36 hectares). The entire field was not sprayed with 2,4-D. The field area sprayed was from the weather tower towards the east and one pass all the way around the field. Weather Conditions: Mostly cloudy with air temperature 72 degrees F. Relative Humidity was 52%. Wind was out of the west at 0-3 mph averaging calm. Time of application was between 4:30 p.m. and 5:30 p.m. As I was finishing spraying the field a light rain started. Equipment Used: JD 5220 tractor traveling 5.0-6.0 mph (gear C1, 1600-1800 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 25-35 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Weeds prevalent: Shepherd's-purse (Capsella bursa-pastoris), common chickweed (Stellaria media), henbit (Lamium amplexicaule) and dandelion (Taraxacum officinale).

2006-11-09 Planting Planted winter wheat, Pioneer 25R37 treated seed, in the Biodiversity study. Planted with the JD 5220 tractor and JD 450 conventional 10' drill. Planting depth was 1 inch.

2004-05-06 Herbicide Application Sprayed the N-rate studies, irrigated and non-irrigated, ECB study, and the extra area east of the 2,4-D study and west of the LTER main drive. Purpose of Application: Control of annual broadleaves and grass weeds. Dual II Magnum (s-metolachlor (+ safener)) was applied at 1.33 pts/A, (1.56L/ha). Callisto (mesotrione) was applied at 6 oz/A (0.44L/ha), and Atrazine 4L (atrazine) was applied at 1 lbs/A (1.12 kg/ha). Weather Conditions: Mostly cloudy with air temperature 80 degrees F. Relative Humidity was 53%. Wind was out of the west at 0-6 mph's averaging 2 mph's. Time of application was between 3:00 p.m. and 4:00 p.m. Equipment Used: JD 5220 tractor traveling 4.0-5.0 mph (gear C1, 1400-1600 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A, (187L/ha), was used as the carrier. Sprayed preemergence. Weeds prevalent: None.

2004-08-21 Harvest August 21, 2004 mowed all reps of T6 plots, reps: 1, 2, 3, 4, 5, and 6. August 23, 2004 tedded all the T6 plots, reps: 1, 2, 3, 4, 5, and 6. August 25, 2004 raked alfalfa in five of the six reps of T6 plots, reps: 1, 2, 3, 4, and 5. We did not rake rep 6 because we wanted to see what things looked like if we did nt rake things and recieved rain. The alfalfa in T6 plots was to wet to bale this afternoon and we were expecting rain tonight so we wanted to see if keeping the alfalfa all spread out (tedded) helps/or not to dry faster after a rain. August 27, 2004 raked alfalfa in all reps of T6 plots. August 31, 2004 Bob A. raked alfalfa in all reps of T6 plots. August 31, 2004 because of the rain we received this past week the alfalfa was no good so Mick chopped all the alfalfa back onto the T6 plots. We did not weigh the alfalfa from the T6 plots we just wanted the alfalfa off the plots. We did not want to add the organic matter back onto the plots so we chopped the alfalfa and removed the alfalfa from the plots.

2004-08-21 Harvest August 21, 2004 mowed all reps of T6 plots, reps: 1, 2, 3, 4, 5, and 6. August 23, 2004 tedded all the T6 plots, reps: 1, 2, 3, 4, 5, and 6. August 25, 2004 raked alfalfa in five of the six reps of T6 plots, reps: 1, 2, 3, 4, and 5. We did not rake rep 6 because we wanted to see what things looked like if we did nt rake things and recieved rain. The alfalfa in T6 plots was to wet to bale this afternoon and we were expecting rain tonight so we wanted to see if keeping the alfalfa all spread out (tedded) helps/or not to dry faster after a rain. August 27, 2004 raked alfalfa in all reps of T6 plots. August 31, 2004 Bob A. raked alfalfa in all reps of T6 plots. August 31, 2004 because of the rain we received this past week the alfalfa was no good so Mick chopped all the alfalfa back onto the T6 plots. We did not weigh the alfalfa from the T6 plots we just wanted the alfalfa off the plots. We did not want to add the organic matter back onto the plots so we chopped the alfalfa and removed the alfalfa from the plots.

2004-08-21 Harvest August 21, 2004 mowed all reps of T6 plots, reps: 1, 2, 3, 4, 5, and 6. August 23, 2004 tedded all the T6 plots, reps: 1, 2, 3, 4, 5, and 6. August 25, 2004 raked alfalfa in five of the six reps of T6 plots, reps: 1, 2, 3, 4, and 5. We did not rake rep 6 because we wanted to see what things looked like if we did nt rake things and recieved rain. The alfalfa in T6 plots was to wet to bale this afternoon and we were expecting rain tonight so we wanted to see if keeping the alfalfa all spread out (tedded) helps/or not to dry faster after a rain. August 27, 2004 raked alfalfa in all reps of T6 plots. August 31, 2004 Bob A. raked alfalfa in all reps of T6 plots. August 31, 2004 because of the rain we received this past week the alfalfa was no good so Mick chopped all the alfalfa back onto the T6 plots. We did not weigh the alfalfa from the T6 plots we just wanted the alfalfa off the plots. We did not want to add the organic matter back onto the plots so we chopped the alfalfa and removed the alfalfa from the plots.

2004-09-04 Herbicide Application Sprayed wheat stubble to keep weeds from producing seeds. Only sprayed T1 reps: 5, 4, 2, and 3. The other reps of T1 did not have enough prennianl weeds growing so we decided not to spray them. We also flail mowed T1 plots on September 8 - 10, 2004. Flail mowed with the JD 6400 tractor and JD 115 flail mower.

2004-09-30 Herbicide Application Sprayed all east - west alleyways in the LTER. The alleyways were uneven and rough so we wanted to till them up and level them off. Not sure what date we planted grass?

2002-04-26 Herbicide Application Sprayed burndown on all replications of treatment 2 on the main site. Roundup Ultra Max (glyphosate) was applied at 26 oz/A (a total of 3 gals of product was applied on 15 acres). Ammonium sulfate (AMS) was added at 17 lbs/100 gals of water (a total of 50 lbs of AMS was used with 300 gals of water). Water was used as the carrier applied at 20 gals/A. Weather Conditions: Sunny with air temperature at 54 degrees F. Relative Humidity was 28%. Wind was out of the west at 3-10 MPH averaging 8 MPH. Application was applied between 4:30-6:30 p.m.

2002-05-11 Herbicide Application Sprayed preemergence herbicide on all replications of treatments 1 and 2 (total area 30 acres) on the main site and the Nitrogen Rate Study (total area 1.1 acres); Dual II Magnum (S-metolachlor (+ safener)) was applied at 1.33 pts/A (a total of 5 gals of product was applied on 31.1 acres); Callisto (mesotrione)was applied at 6 oz/A (a total of 1.4 gals (180 ozs) of product was applied on 31.1 acres); Atrazine 4L (atrazine)was applied at l lb/A (a total of 7.5 gals of product was applied on 31.1 acres); Water was used as the carrier applied at 19 gals/A; Weather Conditions: Cloudy with air temperature ranging from 40-48 degrees F. Relative Humidity was 38%. Wind was out of the southeast at 0-5 MPH averaging 0 MPH; Time of application was between 10:00 a.m. and 2:30 p.m.

2014-05-05 Herbicide Application Sprayed the GLBRC Main Site G1 treatment all replications for preplant burndown weed control using Roundup PowerMax, 2,4D-LV-4 and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 was applied at 1 pt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start cloudy with air temperature of 53 degrees F. Relative Humidity was 53 %. Wind was from EENE 1-8 mph with 10 min avg. of 6 mph. End of application 52 degrees F, Humidity 60 %, winds calm out of the east and partly sunny. Time of application was 4:15 p.m. - 8:00 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-05 Herbicide Application Sprayed the Interaction no-till treatment plots for preplant burndown weed control using Roundup PowerMax, 2,4D-LV-4 and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 was applied at 1 pt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start cloudy with air temperature of 53 degrees F. Relative Humidity was 53 %. Wind was from EENE 1-8 mph with 10 min avg. of 6 mph. End of application 52 degrees F, Humidity 60 %, winds calm out of the east and partly sunny. Time of application was 4:15 p.m. - 8:00 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-05 Herbicide Application Sprayed the LTER T8-NT treatment plots for preplant burndown weed control using Roundup PowerMax, 2,4D-LV-4 and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 was applied at 1 pt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start cloudy with air temperature of 53 degrees F. Relative Humidity was 53 %. Wind was from EENE 1-8 mph with 10 min avg. of 6 mph. End of application 52 degrees F, Humidity 60 %, winds calm out of the east and partly sunny. Time of application was 4:15 p.m. - 8:00 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-05 Herbicide Application Sprayed the LTER Main Site Fertility Gradient both irrigated and non irrigated plots for preplant burndown weed control using Roundup PowerMax, 2,4D-LV-4 and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 was applied at 1 pt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start cloudy with air temperature of 53 degrees F. Relative Humidity was 53 %. Wind was from EENE 1-8 mph with 10 min avg. of 6 mph. End of application 52 degrees F, Humidity 60 %, winds calm out of the east and partly sunny. Time of application was 4:15 p.m. - 8:00 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-05 Herbicide Application Sprayed the LTER Main Site Lysimeter field for preplant burndown weed control using Roundup PowerMax, 2,4D-LV-4 and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, 2,4-D LV4 was applied at 1 pt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start cloudy with air temperature of 53 degrees F. Relative Humidity was 53 %. Wind was from EENE 1-8 mph with 10 min avg. of 6 mph. End of application 52 degrees F, Humidity 60 %, winds calm out of the east and partly sunny. Time of application was 4:15 p.m. - 8:00 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-06 Herbicide Application Sprayed the LTER Main Site T2's all replications in order of (5, 2, 3, 4, 1, 6) for preplant burndown weed control using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start mostly sunny with air temperature of 57 degrees F. Relative Humidity was 50 %. Wind was from NE 1-8 mph with 10 min avg. of 7 mph. End of application 63 degrees F, Humidity 42 %, winds 5-10 mph out of the NE and partly sunny. Time of application was 12:40 p.m. - 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-08 Herbicide Application Sprayed the LTER CE study, treatments C1, C2, C3 all plots for preplant burndown weed control using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start mostly sunny with air temperature of 59 degrees F. Relative Humidity was 74 %. Wind was from NE 1-5 mph SE with 10 min avg. of 2 mph. End of application 74 degrees F, Humidity 61 %, winds 5-10 mph out of the SW and partly sunny. Time of application was 9:35 a.m. - 11:35 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-08 Herbicide Application Sprayed the GLBRC Bait Crop, (5 plots in rep 6, brown house, and borders) for preplant burndown weed control using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start mostly sunny with air temperature of 59 degrees F. Relative Humidity was 74 %. Wind was from NE 1-5 mph SE with 10 min avg. of 2 mph. End of application 74 degrees F, Humidity 61 %, winds 5-10 mph out of the SW and partly sunny. Time of application was 9:35 a.m. - 11:35 a.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2002-05-24 Herbicide Application May 24, 2002 Dual II Magnum, Callisto, and Atazine was applied preemergence to the corn in the European Corn Borer Study and the second Nitrogen Rate Study. Purpose of application: Control of annual grass and broadleaf weeds. This application was a broadcast preemergence herbicide. Dual II Magnum (S-metolachlor (+ safener)) was applied at 1.33 pts/A, (1.56 L/ha), total amount of Dual II Magnum used 7 pts (3.32 L). Callisto (mesotrione) was applied at 6 oz/A, total amount of Callisto used 30 ozs (0.89 L). Atrazine 4L (atrazine) was applied at l lb/A, total amount of atrazine used: 5 qts (4.74 L). Total area covered: 3.1 acres (1.26 hectares). Weather Conditions: Air temperature: 62 degrees F. Wind was out of the northwest at 0-8 mph, with an average of 3 mph. Relative Humidity: 52%. Time of application: 12:00 noon - 1:00 p.m. Equipment Used: JD 5220 tractor traveling 4.5 mph, gear B3 2000 rpm, with a pull type Top Air sprayer equipped with a PTO driven centrifugal sprayer pump and a Raven 440 automatic carrier control unit. Nozzles used: Turbo TeeJet 11003 spaced 15 inches apart with a total boom length of 30 feet. Application pressure: 12-14 psi. Carrier used: Water applied at 20 gals/A (187L/ha).

2014-05-21 Herbicide Application Sprayed Interaction plots for pre-emergence weed control where corn was planted with Lexar at a rate of 3 qts/A. Weather conditions at the start 10:25 a.m. were mostly sunny 65 degrees F, 94% humidity with 3-5 mph wind out of W. Conditions at the end 7:10 p.m. were mostly sunny, 79 degrees F, 31% humidity, and 5-8 mph winds out of the W. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-21 Herbicide Application Sprayed GLBRC bait crop, the north edge (south half) and two plots in rep 6 (first 2 east of miscanthus) for pre-emergence weed control where corn was planted with Lexar at a rate of 3 qts/A. Weather conditions at the start 10:25 a.m. were mostly sunny 65 degrees F, 94% humidity with 3-5 mph wind out of W. Conditions at the end 7:10 p.m. were mostly sunny, 79 degrees F, 31% humidity, and 5-8 mph winds out of the W. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-21 Herbicide Application Sprayed LTER T8-NT's for pre-emergence weed control where corn was planted with Lexar at a rate of 3 qts/A. Weather conditions at the start 10:25 a.m. were mostly sunny 65 degrees F, 94% humidity with 3-5 mph wind out of W. Conditions at the end 7:10 p.m. were mostly sunny, 79 degrees F, 31% humidity, and 5-8 mph winds out of the W. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-21 Herbicide Application Sprayed LTER CE study treatments C1 and C2 for pre-emergence weed control where corn was planted with Lexar at a rate of 3 qts/A. Weather conditions at the start 10:25 a.m. were mostly sunny 65 degrees F, 94% humidity with 3-5 mph wind out of W. Conditions at the end 7:10 p.m. were mostly sunny, 79 degrees F, 31% humidity, and 5-8 mph winds out of the W. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-21 Herbicide Application Sprayed LTER Main Site Irrigated Fertility Gradient (everything north of trailer and east of trailer) for pre-emergence weed control where corn was planted with Lexar at a rate of 3 qts/A. Weather conditions at the start 10:25 a.m. were mostly sunny 65 degrees F, 94% humidity with 3-5 mph wind out of W. Conditions at the end 7:10 p.m. were mostly sunny, 79 degrees F, 31% humidity, and 5-8 mph winds out of the W. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-21 Herbicide Application Sprayed LTER Lysimeter Field for pre-emergence weed control where corn was planted with Lexar at a rate of 3 qts/A. Weather conditions at the start 10:25 a.m. were mostly sunny 65 degrees F, 94% humidity with 3-5 mph wind out of W. Conditions at the end 7:10 p.m. were mostly sunny, 79 degrees F, 31% humidity, and 5-8 mph winds out of the W. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-21 Herbicide Application Sprayed LTER Main Site treatment T2 all replications (6, 1, 2, 3, 4, 5) for pre-emergence weed control where corn was planted with Lexar at a rate of 3 qts/A. Weather conditions at the start 10:25 a.m. were mostly sunny 65 degrees F, 94% humidity with 3-5 mph wind out of W. Conditions at the end 7:10 p.m. were mostly sunny, 79 degrees F, 31% humidity, and 5-8 mph winds out of the W. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-23 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. Plots (G6 rep1, G5 rep2, G6 rep 2, G9 rep 2, G6 rep 3), were fertilized Saturday morning. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Each treatment plot was divided into two areas, an unfertilzed microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. Conditions: At the start sunny, 66 degrees F, 52% Humidity with winds 1-6 mph N. Conditions at the end 65 degrees F, 52 % humidity winds 1-6 mph WNW. Time of application 2:30 - 7:30 p.m. Equipment Used: JD 5220 tractor traveling 3.5 mph (gear B2, 1650 rpm) with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11005 nozzles were used, spaced 30 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2014-05-23 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatment G10, MICROPLOT area only, all replications. Treatment G10 microplots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs / gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Each treatment plot was divided into two areas: fertilized microplot areas 15' wide located on the west side of treatment plots [G10R2, G10R3, G10R4, and G10R5] and located on the east side of the treatment plot G10R1; unfertilized areas which were the remaining 76.86' on the east side of the plots [G10R2, G10R3, G10R4, and G10R5] and located on the west side of treatment plot G10R1. The fertilized 15' microplot received 50 lbs N/A. The unfertilized 76.86' was NOT fertilized. Conditions: At the start sunny, 66 degrees F, 52% Humidity with winds 1-6 mph N. Conditions at the end 65 degrees F, 52 % humidity winds 1-6 mph WNW. Time of application 2:30 - 7:30 p.m. Equipment Used: JD 5220 tractor with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart. Purpose of Application: Supply nitrogen to the treatment plots.

2014-05-24 Fertilizer Application Fertilized LTER Cellulsic Biofuels Experiment (CE Study) treatment CE4 and CE6, all replications. Plots received 50 lbs N/A with 17 gallons/A 28% N liquid fertilizer. Treatments CE4: (plots 110, 203, 302, 404); and CE6: (plots 111, 204, 310, 406).

2014-05-24 Herbicide Application Sprayed LTER Main Site treatment T3 all replications (2, 3, 4, 5, 1, 6) for pre-emergence weed control where corn was planted. Sprayed with Dual II Magnum at a rate of 1.33 pts/A. Weather conditions at the start 10:00 a.m. were mostly sunny 63 degrees F, 63% humidity with calm wind out of NE. Conditions at the end 8:00 p.m. were mostly sunny, 70 degrees F, 43% humidity, and 1-5 mph winds out of the NW. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-24 Herbicide Application Sprayed GLBRC Main Site treatment G1 all replications (1, 4, 2, 5, 3,) for pre-emergence weed control where corn was planted. Sprayed with Lexar at 3 qts/A and Roundup Power Max at 21 oz/A and ammonium sulfate at 3.4 lbs/A. Weather conditions at the start 10:00 a.m. were mostly sunny 63 degrees F, 63% humidity with calm wind out of NE. Conditions at the end 8:00 p.m. were mostly sunny, 70 degrees F, 43% humidity, and 1-5 mph winds out of the NW. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-24 Herbicide Application Sprayed LTER Main Site treatment T1 all replications (6, 2, 3, 4, 5, 1) for pre-emergence weed control where corn was planted. Sprayed with Lexar at 3 qts/A and Roundup Power Max at 21oz/A and ammonium sulfate at 3.4 lbs/A. Weather conditions at the start 10:00 a.m. were mostly sunny 63 degrees F, 63% humidity with calm wind out of NE. Conditions at the end 8:00 p.m. were mostly sunny, 70 degrees F, 43% humidity, and 1-5 mph winds out of the NW. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-23 Fertilizer Application Fertilized GLBRC Main Site Switchgrass Fertility Study (SWF: N-rate), all treatments and all replications. Fertilizer applied as 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN) (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate).The amounts of nitrogen applied were as follows: SWF1 (108, 201, 307, 408) 0 lbs/A; SWF2 (104, 206, 308, 407) 25 lbs N/A (8.4 gal/A); SWF3 (102, 202, 302, 406) 50 lbs N/A (16.7 gal/A); SWF4 (105, 208, 305, 405) 75 lbs N/A (25.1 gal/A); SWF5 (101, 205, 304, 404) 100 lbs N/A (33.5 gal/A); SWF6 (107, 207, 301, 403) 125 lbs N/A (41.8 gal/A); SWF7 (106, 204, 306, 402) 150 lbs N/A (50.2 gal/A); SWF8 (103, 203, 303, 401) 175 lbs N/A (58.6 gal/A). All passes except for F2 & F3 applications were made from the south alleyway across to the north side, doing blocks 1 & 3 at a time and then 2 & 4. Conditions: At the start sunny, 66 degrees F, 52% Humidity with winds 1-6 mph N. Conditions at the end 65 degrees F, 52 % humidity winds 1-6 mph WNW. Time of application 2:30 - 7:30 p.m. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. The sprayer was controlled by a John Deere Rate Controller. Nozzles were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer; 11 nozzles were used on the boom. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 15-20 psi. TeeJet 8001 nozzles were used for the SWF2 level. TurboTeeJet 11002 nozzles were used for the SWF3. TurboTeeJet 11003 nozzles were used for the SWF4, SWF5, levels. TurboTeeJet 11005 nozzles were used for the SWF7, SWF7 and SWF8 levels.

2014-05-31 Herbicide Application Sprayed the GLBRC Main Site G2, G3, G4 treatment all replications for post - plant burndown of cover crops and grasses using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start sunny with air temperature of 74 degrees F, humidity was 53 % with 5 mph winds ESE. End of application sunny, 86 degrees F, humidity 33 %, with calm winds Time of application was 10:20 a.m. - 4:15 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-31 Herbicide Application Sprayed LTER Main Site Non - Irrigated Fertility Gradient (everything West of trailer) for post-emergence weed control where corn was planted with Lexar at a rate of 3 qts/A, Roundup Power Max at 16 oz/A, and Ammonium Sulfate at 3.4 lbs/A. Weather conditions: At the start sunny with air temperature of 74 degrees F, humidity was 53 % with 5 mph winds ESE. End of application sunny, 86 degrees F, humidity 33 %, with calm winds Time of application was 10:20 a.m. - 4:15 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-05-31 Herbicide Application Sprayed GLBRC Bait Crop (East side inside pass, South side both inside and outside pass) for pre - emergence weed control where corn was planted with Lexar at a rate of 3 qts/A, Roundup Power Max at 16 oz/A, and Ammonium Sulfate at 3.4 lbs/A. Weather conditions: At the start sunny with air temperature of 74 degrees F, humidity was 53 % with 5 mph winds ESE. End of application sunny, 86 degrees F, humidity 33 %, with calm winds Time of application was 10:20 a.m. - 4:15 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-06-13 Herbicide Application Sprayed the LTER CE study, treatment C3 all plots for post-plant burndown weed control using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 22 oz/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start 8:15 a.m. mostly sunny with air temperature of 55 degrees F. Relative Humidity was 63 %. Wind was from the W at 4-7 mph. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha),

2014-06-14 Herbicide Application Sprayed the LTER CE study, treatment C1&2 all plots for post-Emergence grass control using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 22 oz/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start 8:00 a.m. sunny with air temperature of 50 degrees F. Relative Humidity was 90 %. Wind was calm from the NE at 0 mph. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha),

2014-06-14 Herbicide Application Sprayed the GLBRC bait crop where soybeans were planted (3rd-5th plots east of miscanthus in rep 6, brown house, 1 30ft strip around the entire 3 sides of the GLBRC) for post plant burndown using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 22 oz/acre, and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start 8:00 a.m. sunny with air temperature of 50 degrees F. Relative Humidity was 90 %. Wind was calm from the NE at 0 mph. At the end 10:00 a.m. 64 degrees F 56 % humidity, sunny with calm NW winds. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha),

2014-06-26 Herbicide Application Sprayed the GLBRC Main Site G2, G3, G4 treatment all replications for post emergence weed control using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) (Glyphosate: N-(phosphonomethyl) glycine, in the form of its potassium salt, 48.7%) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 3.4 lbs/acre. Weather conditions: At the start sunny with air temperature of 74 degrees F, humidity was 65 % with calm winds NE. End of application sunny, 78 degrees F, humidity 78 %, with calm NE winds Time of application was 2:00 p.m. - 3:30 p.m. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2014-07-24 Mechanical Weed Control Mowed between tree rows in the GLBRC Main Site G8 plots, all reps in the order of (5, 4, 3, 1, 2). Purpose was to decrease weed population to insure the poplar trees receive adequate water to grow properly. Equipment used was the JD 5220 and the JD 6' brushog.

2015-03-18 Planting Frost seeded red clover in 2015 LTER Biodiversity Study winter wheat plots. Red clover was seeded into treatment B5 (114, 217, 312, 405), B8 (118, 214, 310, 408), B17 (116, 211, 302, 417) for a total of 12 plots all of which had winter wheat planted in November of 2014. Clover was seeded at 12.56 lbs/A with Gandy air seeder with a 15' boom. Seeded at 9:00 a.m. - 10:00 a.m. weather conditions at this time were clear and sunny, temperature was 31 degrees F with calm winds. For calibration figures, seed tag information, and other information please see PDF attachment.

2015-05-02 Herbicide Application Sprayed GLBRC Main Site treatment G1 all replications (5, 4, 1, 2, 3) for pre-emergence weed control where corn was planted. Sprayed with Lexar at 3 qts/A and Roundup Power Max at 22 oz/A and ammonium sulfate at 3.4 lbs/A. Weather conditions at the start 11:40 a.m. were sunny 66 degrees F, 40% humidity with 4 mph West South West wind. Conditions at the end 2:00 p.m. were sunny, 71 degrees F, 28% humidity, and 4 mph wind South East.

2015-05-02 Herbicide Application Sprayed GLBRC Bait Crop: North and South perimeter bait crop where corn was planted (middle 30 ft on south side and north 30 ft on North side), third plot from the west in rep 6, and behind the brown house for pre-emergence weed control where corn was planted. Sprayed with Lexar at 3 qts/A and Roundup Power Max at 22 oz/A and ammonium sulfate at 3.4 lbs/A. Weather conditions at the start 11:40 a.m. were sunny 66 degrees F, 40% humidity with 4 mph West South West wind. Conditions at the end 2:00 p.m. were sunny, 71 degrees F, 28% humidity, and 4 mph wind South East.

2015-05-13 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatment G10, MICROPLOT area only, all replications. Treatment G10 microplots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs / gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Each treatment plot was divided into two areas: fertilized microplot areas 15' wide located on the west side of treatment plots [G10R2, G10R3, G10R4, and G10R5] and located on the east side of the treatment plot G10R1; unfertilized areas which were the remaining 76.86' on the east side of the plots [G10R2, G10R3, G10R4, and G10R5] and located on the west side of treatment plot G10R1. The fertilized 15' microplot received 50 lbs N/A. The unfertilized 76.86' was NOT fertilized. Conditions: At the end sunny, 56 degrees F, 60% Humidity with average wind of 5 mph out of the east. Equipment Used: JD 5220 tractor with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Chafer stream bars were used, spaced 15 inches apart. Purpose of Application: Supply nitrogen to the treatment plots.

2015-05-13 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Each treatment plot was divided into two areas, an unfertilzed microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. Conditions: At the end sunny, 56 degrees F, 60 % Humidity with 4 mph avg. east winds. Equipment Used: JD 5220 tractor with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Chafer stream bars were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2015-05-13 Fertilizer Application Fertilized GLBRC Main Site Switchgrass Fertility Study (SWF: N-rate), all treatments and all replications. Fertilizer applied as 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN) (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). The amounts of nitrogen applied were as follows: SWF1 (108, 201, 307, 408) 0 lbs/A; SWF2 (104, 206, 308, 407) 25 lbs N/A (8gal/A); SWF3 (102, 202, 302, 406) 50 lbs N/A (17 gal/A); SWF4 (105, 208, 305, 405) 75 lbs N/A (25 gal/A); SWF5 (101, 205, 304, 404) 100 lbs N/A (34 gal/A); SWF6 (107, 207, 301, 403) 125 lbs N/A (42 gal/A); SWF7 (106, 204, 306, 402) 150 lbs N/A (50 gal/A); SWF8 (103, 203, 303, 401) 175 lbs N/A (59 gal/A). All passes were made from the south alleyway across to the north side, doing blocks 1 & 3 at a time and then 2 & 4. Conditions: At the end sunny, 56 degrees F, 60% Humidity with avg.winds 4 mph out of the east. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. The sprayer was controlled by a John Deere Rate Controller. Chafer stream bars were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer; 11 nozzles were used on the boom. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 17-40 psi. 1.0 Orifice's were used in the chafer stream bars for SWF2 (17psi.C1), SWF3 (40psi B2), SWF4 (40psi B1); and 1.8 orifice's were used in the chafer stream bars on SWF5 (30psi B3), SWF6 (30psi B2), SWF7 (20psi B1),and SWF8 (30psi B1).

2015-05-14 Fertilizer Application Applied 28% (28-0-0) nitrogen fertilizer at 17 gals/A (50.78 lbs N/A) to the switchgrass in fields L2 at Lux Arbor and M3 at the Marshall Farm. One gallon of 28% fertilizer weighs 10.67 lbs. so 50.78 lbs N/A was applied. Weather conditions at start 9:15 a.m. mostly sunny, 49.6 degrees F. 69% humidity, wind out of the southeast-east 6-12 mph, Avg. 8 mph. Weather conditions at 5:00 p.m. cloudy 60 degrees F. 41% humidity, wind out the south east 5-12 mph, Avg. 6 mph. Weather conditions at the end of application 8:00 p.m. cloudy, 58 degrees F. 45% humidity, wind out the the south east 2-6 mph, Avg. 3 mph.

2015-05-21 Fertilizer Application Fertilized LTER Cellulsic Biofuels Experiment (CE Study) treatment CE4 and CE6, all replications. Plots received 50 lbs N/A with 17 gallons/A 28% N liquid fertilizer. Treatments CE4: (plots 110, 203, 302, 404); and CE6: (plots 111, 204, 310, 406).

2015-05-22 Herbicide Application Sprayed the LTER Main Site treatment T1 soybeans all replications (6, 5, 1, 4, 2, 3) with Valor XLT (EPA. 59639-117) a pre-emerge herbicide. Rate of application was 3.5 oz/A. Pre-emerge application was done to prevent weed growth in soybeans. Weather conditions at the start 9:45 a.m. sunny, 56 degrees F. 28% humidity, 5-10 mph North - North East wind. Conditions at end 7:45 p.m. sunny, 63 degrees F. 29% humidity, 5-10 mph North - North West winds.

2015-05-22 Herbicide Application Sprayed the LTER Main Site treatment T2 soybeans all replications (5, 2, 3, 6, 4, 1), LTER Fertility Gradient, Lysimeter field with Roundup PowerMax (EPA.524-549) and Valor XLT (EPA. 59639-117) a pre-emerge herbicide. Rate of application was Roundup PowerMax 32 oz/A, Ammonium Sulfate 3.4 lbs/A, Valor XLT 3.5 oz/A, and drift agent and anti-foam 1 oz/100 gallons. Pre-emerge/post plant burndown application was done to burn down any weeds and also prevent weed growth in soybeans. Weather conditions at the start 9:45 a.m. sunny, 56 degrees F. 28% humidity, 5-10 mph North - North East wind. Conditions at end 7:45 p.m. sunny, 63 degrees F. 29% humidity, 5-10 mph North - North West winds.

2015-06-03 Herbicide Application Sprayed the GLBRC Main Site G1, G2, G3, G4 treatment all replications for (G1) post emergence / (G2-G4) post plant burndown of cover crops and grasses using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Also sprayed all of the bait crop in the GLBRC. Weather conditions: At the start 9:00 a.m. sunny with air temperature of 58 degrees F, humidity was 77 % with 4 mph east winds. End of application 3:40 p.m. sunny, 73 degrees F, humidity 53 %, with 4-8 mph avg 5mph south east winds. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11002 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 15 gals/A, was used as the carrier.

2015-06-03 Herbicide Application Sprayed the LTER T8nt, interaction, and CES 1,2,&3 treatments all replications for burndown using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Also sprayed all of the bait crop in the GLBRC. Weather conditions: At the start 9:00 a.m. sunny with air temperature of 58 degrees F, humidity was 77 % with 4 mph east winds. End of application 3:40 p.m. sunny, 73 degrees F, humidity 53 %, with 4-8 mph avg 5mph south east winds. Equipment Used: JD 5220 tractor traveling 4.8 mph (gear C1, 1500 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Turbo TeeJet 11002 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 15 gals/A, was used as the carrier.

2015-07-01 Herbicide Application Sprayed the GLBRC Main Site G2, G4 treatment all replications the first two corn plots on the east side of rep 6, and the middle pass of corn on the east perimeter bait crop with a post emergence application of Roundup PowerMax, and ammonium sulfate, and (corral drift agent and anti foam product). Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 10:40 a.m. mostly sunny with air temperature of 58 degrees F, humidity was 89 % with 4 mph north north west winds. End of application 4:30 p.m. mostly sunny, 68 degrees F, humidity 59 %, with 4-8 mph north-north west winds.

2015-07-01 Herbicide Application Sprayed the LTER Main Site treatment T3 all replications (6, 1, 2, 3, 4, 5)and CE study corn plots (CES1, CES3) with a post emergence application of Roundup PowerMax, and ammonium sulfate, and (corral drift agent and anti foam product 1.5oz/A). Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 10:40 a.m. mostly sunny with air temperature of 58 degrees F, humidity was 89 % with 4 mph north north west winds. End of application 4:30 p.m. mostly sunny, 68 degrees F, humidity 59 %, with 4-8 mph north-north west winds.

2015-07-23 Herbicide Application Sprayed the LTER Main Site treatments T1, and T2 all replications for post emergence weed control using an application of Roundup PowerMax, and ammonium sulfate, and (corral drift agent and anti foam product). Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 10:30 a.m. mostly cloudy with air temperature of 68 degrees F, humidity was 77 % with 1-4 mph south west winds. End of application 5:00 p.m. mostly sunny, 81 degrees F, humidity 55 %, with 1-5 mph west winds.

2015-07-28 Herbicide Application Sprayed the GLBRC Main Site G3 treatment all replications and all soybean bait crop for post emergence weed control using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Also sprayed all of the bait crop in the GLBRC. Weather conditions: At the start 2:20 p.m. sunny with air temperature of 88 degrees F, humidity was 48 % with 1-4 mph south south east winds. End of application 4:20 p.m. sunny, 90 degrees F, humidity 48 %, with 1-5 mph south east winds.

2015-07-29 Herbicide Application Sprayed soybeans in the LTER irrigated and non irrigated fertility gradient, lysimeter field, CES treatment 2 (soybeans), T8nt plots, and the interaction plots for post emergence weed control using Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 10:50 a.m. overcast with air temperature of 80 degrees F, humidity was 79 % with 3-5 mph west winds. End of application 3:45 p.m. Partly sunny, 83 degrees F, humidity 76 %, with 4-6 mph north west w

2015-08-21 Herbicide Application Sprayed the LTER Main Site treatment T6, all replications for burn down using an application of Roundup PowerMax, ammonium sulfate, and 2,4-D LV4. Roundup Power Max (EPA Reg. No. 524-549) was applied at 44 oz/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier, and 2,4-D LV4 was applied at 1 qt/A. Alfalfa stand was thin so it will be planted to winter wheat fall of 2015 and then reseeded to alfalfa the following fall (2016). Weather conditions: At the start 12:40 p.m. sunny, 74 degrees F, humidity was 58 % with 2-4 mph west south west winds. End of application 3:00 p.m. sunny, 77 degrees F, humidity 53 %, with 2-8 mph south west winds.

2015-10-05 Herbicide Application Sprayed the LTER Main Site treatment T6, all replications for burn down using an application of Roundup PowerMax and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 2 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 2:00 p.m. overcast, 56 degrees F, humidity was 98 % with 2-4 mph north - north east winds. End of application 6:00 p.m. sunny, 61 degrees F, humidity 89 %, with 1-2 mph north - north east winds.

2016-03-22 Fertilizer Application Fertilized LTER N-Rate Study treatments 2-9. Plots were fertilized with 28% U.A.N. nitrogen fertilizer at a rate of 7 gallons per acre. A gallon of 28% fertilizer weighs 10.67 lbs, so 20 pounds of actual N was applied. Applied with Top Air Sprayer equipped with chafer streamer bars with 1.0 orifice. Do to low volume only half the boom was on and every 15 foot plot was covered individually. Start 8:11 a.m. weather was mostly sunny 40 degrees F. 5-8 mph south wind. End of application 11:25 a.m. mostly sunny 51 degrees F. 3-6 mph south west wind, 46% humidity.

2016-03-21 Fertilizer Application Fertilized LTER Main Site treatments T1, T2, and T3 winter wheat plots all replications and lysimeter field. Plots were fertilized with 28% U.A.N. nitrogen fertilizer at a rate of 10 gallons per acre. A gallon of 28% fertilizer weighs 10.67 lbs, so 30 pounds of actual N was applied. Applied with Top Air Sprayer equipped with chafer streamer bars with 1.0 orifice. Weather conditions at 10:00 a.m. Sunny, Temperature: 38 degrees F, Humidity: 53%, Wind: coming out of the Northwest at 1-4 mph (average of 2 mph). Weather conditions at 1:00 p.m. Sunny, Temperature: 39 degrees F, Humidity: 44%, Wind: coming out of the Northwest at 7.0 mph. (10 minute average). Weather conditions at 2:30 p.m. Sunny, Temperature: 42 degrees F, Humidity: 36%, Wind: coming out of the Northwest at 7.0 mph. (10 minute average). Dew Point: 17 degrees F. Weather conditions at 6:00 p.m. Sunny, Temperature: 43 degrees F, Humidity: 43%, Wind: coming out of the West-Northwest at 1-10 mph. Dew Point: 22 degrees F.

2016-03-23 Planting Frost seeded LTER Main Site Treatment T4 all replications, and the Biodiversity study treatments b4 (104, 220, 307, 404), b7 (105, 219, 305, 407), b17 (116, 211, 302, 417), with Medium Red Clover at 12 lbs/A. Clover seed had a coating that was 34% of the seed weight. A pure live seed calculation was done to calculate amount of seed per acre needed to achieve the 12 lbs/A of pure live seed. The calculation equaled 20 lbs/A seeding rate to achieve the 12 lbs/A of pure seed (see papers attached). Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 22.5 inches. Gandy motor setting was 1.78 with the red metering wheels in and the gates on the Gandy were in the first position. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Start of application was 8:15 a.m. weather was cloudy 41 degrees F 3-7 mph east north east wind, 81% humidity. End of application 1:20 p.m. cloudy 46 degrees F 6-10 mph east north east wind, 63% humidity. Total amount of seed added was 359 pounds with 33 pounds remaining at the end. so 326 pounds of seed applied over 16 acres equals 20.4 lbs/A seeding rate. This should give close to a 12 lbs/A pure live seeding rate.

2016-03-29 Herbicide Application Sprayed LTER Main Site Treatment T6 all replications (6,1,2,4,3,5), for post emergence weed control in winter wheat. Plots sprayed with Harmony Extra at .75 oz/A and Preference non-ionic surfactant at 0.25% v/v (1 qt/100 gal carrier). Time of application was 2:20 - 4:30 p.m. Weather conditions at start, sunny 52 degrees F, 30% humidity, 2-4 mph north east winds. Conditions at end, sunny 55 degrees F, 32% humidity, winds random 2-5 mph. Equipment used: JD 5220 tractor traveling 5 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Nozzles were Turbo Teejet 11003 flat fans spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A was used as the carrier.

2016-04-15 Herbicide Application Sprayed LTER Main Site Treatment T3, all replications (6, 3, 1, 2, 4, 5) for post emergence weed control in winter wheat. Plots sprayed with a tank mix of Anti-Foaming agent at 1oz/100 gal of carrier, Ammonium Sulfate at 3lbs/A, Powerflex HL at 2 oz/A and Premier non-ionic surfactant at 0.5% v/v (2 qt/100 gal carrier). Time of application was 4:00 - 6:00 p.m. Weather conditions were sunny, temperature was 68°F at start and 70°F at end,34 - 38 % humidity, with winds out of the east south east at 2-9 mph. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Nozzles were Turbo Teejet 11003 flat fans spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A was used as the carrier.

2016-04-16 Herbicide Application Sprayed LTER Main Site Treatment T1, all replications (6, 5, 1, 4, 2, 3), T2 all replications (6, 1, 4, 3, 2, 5), T8nt plots, N-Rate Study, and Lysimeter field for post emergence weed control in winter wheat. Plots sprayed with a tank mix of Anti-Foaming agent at 1oz/100 gal of carrier, Ammonium Sulfate at 3lbs/A, Powerflex HL at 2 oz/A and Premier (90%) non-ionic surfactant at 0.5% v/v (2 qt/100 gal carrier). Time of application was 9:20 a.m. - 5:30 p.m. Weather conditions 9:20 a.m. were sunny, temperature was 54°F 56% humidity 3-5 mph East wind, 11:35 a.m. sunny 63 degrees F 43% humidity 1-2 mph East wind, 12:20 p.m. sunny 72 degrees F 43 % humidity 2-5 mph East, South East wind, 2:30 p.m. sunny 75 degrees F, 27 % humidity, 5-11 mph South East wind, 3:10 p.m. 75 degrees F 28 % humidity 5-10 mph East wind, 5:30 p.m. 76 degrees F, 29 % humidity, 1-7 mph East South East wind. Equipment used: JD 5220 tractor traveling 4.8 mph (gear C1, 1550 rpm) with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Nozzles were Turbo Teejet 11003 flat fans spaced 15 inches apart with a total boom length of 30 feet. 20-30 psi was used. Water applied at 20 gals/A was used as the carrier.

2016-04-22 Fertilizer Application Fertilized LTER Main Site treatment T2 all replications (6, 5, 2, 3, 4, 1) with a blended mixture of ((28-0-0) and (11-0-0-26) (together figured by weight have a fertilizer analysis of 20-0-0-12). The blend (20-0-0-12) was applied at 19 gallons/A, one gallon weighs 10.83 lbs so it provided 41 lbs of N/A, and 25 lbs of sulfur/A. Started spraying at 9:20 a.m. weather conditions were taken at 11:15 a.m. and 12:50 p.m. but did not vary much from 9:20 a.m. At 11:15 it was cloudy 54 degrees F, 98 % humidity 54 degrees F dew point with 0-5 mph north winds. Conditions at 12:50 p.m. it was cloudy 55 degrees F, 99 % humidity with 4-7 mph north winds.

2016-04-24 Fertilizer Application Fertilized LTER Main Site treatment T1 all replications (6, 5, 1, 4, 2, 3), Replications 5&6 were fertilized April 22, 2016. Conditions became to wet and replications 1-4 were finished being fertilized April 24, 2016. Plots were fertilized with a blended mixture of ((28-0-0) and (11-0-0-26) (together figured by weight have a fertilizer analysis of 20-0-0-12). The blend (20-0-0-12) was applied at 19 gallons/A, one gallon weighs 10.83 lbs so it provided 41 lbs of N/A, and 25 lbs of sulfur/A. Weather conditions 8:10 a.m. sunny 48 degrees F, 57% humidity 1-3 mph south south east wind. Conditions 12:42 sunny, 65 degrees F 35% humidity 2-6mph south winds.

2016-03-22 Fertilizer Application Fertilized LTER T8Nt. Plots were fertilized with 28% U.A.N. nitrogen fertilizer at a rate of 10 gallons per acre. A gallon of 28% fertilizer weighs 10.67 lbs, so 30 pounds of actual N was applied. Applied with Top Air Sprayer equipped with chafer streamer bars with 1.0 orifice. Weather conditions mostly sunny 40 degrees F. 5-8 mph south wind. End of application 11:25 a.m. mostly sunny 51 degrees F. 3-6 mph south west wind, 46% humidity.

2016-04-27 Fertilizer Application Fertilized LTER Main Site treatment T3 all replications (5, 4, 2, 1, 3, 6), Plots were fertilized with a blended mixture of ((28-0-0) and (11-0-0-26) (together figured by weight have a fertilizer analysis of ). 11-0-0-26 weighs 11 lbs/gallon so 11 lbs of N/A and 26 lbs of sulfur/A were applied. 28-0-0 weighs 10.67 lbs/gallon so 15 lbs of N/A was applied. Total with the blended mixture 26 lbs of N and 26 lbs of sulfur was applied per acre. Weather conditions 10:25 a.m. partly cloudy 46 degrees F, 73% humidity 5-11 mph north east wind. Conditions 1:31 p.m. partly cloudy, 62 degrees F 29% humidity 2-10 mph north east winds.

2016-04-27 Fertilizer Application Fertilized LTER N-rate study (Irrigated and Non-irrigated fertility gradient study) winter wheat plots. Fertilizer applications were with 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN). A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. Plots F2 and up already were applied with 7 gal/A of 28-0-0 supplying 20 lbs of N/A. This application started at treatment F3 to apply the remaining nitrogen. The amounts of remaining nitrogen applied were as follows: F1 (101, 203, 309, 404, 501, 603, 709, 804) 0 lbs/A; F2 (102, 202, 304, 406, 502, 602, 704, 806) 0 lbs N/A (0 gal/A); F3 (103, 205, 302, 407, 503, 605, 702, 807) 20 lbs N/A (6.7 gal/A); F4 (104, 208, 305, 403, 504, 608, 705, 803) 40 lbs N/A (13.4 gal/A); F5 (105, 204, 306, 401, 505, 604, 706, 801) 60 lbs N/A (20 gal/A); F6 (106, 209, 308, 402, 506, 609, 708, 802) 80 lbs N/A (26.8 gal/A); F7 (107, 201, 307, 408, 507, 601, 707, 808) 100 lbs N/A (33.5 gal/A); F8 (108, 207, 303, 405, 508, 607, 703, 805) 120 lbs N/A (40.2 gal/A); F9 (109, 206, 301, 409, 509, 606, 701, 809) 140 lbs N/A (46.9 gal/A). Guard plots were sprayed at rates comparable to adjacent treatment plots. 1.0 orifices were used f3-f6, 1.8 orifices used f7-f9 Time of application was between 1:30 p.m. and 5:45 p.m. Conditions at 1:30 p.m. were cloudy temperature was 57.4 F, 41% relative humidity with 2-10 mph north east wind. Conditions at 5:45 p.m. were cloudy 62 degrees F, 29 % humidity, with 3-5 mph east wind.

2016-04-24 Fertilizer Application Fertilized LTER Main Site Lysimiter field with a blended mixture of ((28-0-0) and (11-0-0-26) (together figured by weight have a fertilizer analysis of 20-0-0-12). The blend (20-0-0-12) was applied at 19 gallons/A, one gallon weighs 10.83 lbs so it provided 41 lbs of N/A, and 25 lbs of sulfur/A. Weather conditions 8:10 a.m. sunny 48 degrees F, 57% humidity 1-3 mph south south east wind. Conditions 12:42 sunny, 65 degrees F 35% humidity 2-6mph south winds.

2016-04-27 Fertilizer Application Fertilized LTER T8nt all replications, Plots were fertilized with a blended mixture of ((28-0-0) and (11-0-0-26) (together figured by weight have a fertilizer analysis of ). 11-0-0-26 weighs 11 lbs/gallon so 11 lbs of N/A and 26 lbs of sulfur/A were applied. 28-0-0 weighs 10.67 lbs/gallon so 15 lbs of N/A was applied. Total with the blended mixture 26 lbs of N and 26 lbs of sulfur was applied per acre. Conditions 1:31 p.m. partly cloudy, 62 degrees F 29% humidity 2-10 mph north east winds. Conditions at 5:45 p.m. were cloudy 62 degrees F 29% humidity with 3-5 mph east winds.

2016-04-27 Fertilizer Application Fertilized LTER T8nt all replications, Plots were fertilized with 28-0-0 at 12 gallons/A. 28-0-0 weighs 10.67 lbs/gallon so 36 lbs of N/A was applied. Conditions 1:31 p.m. partly cloudy, 62 degrees F 29% humidity 2-10 mph north east winds. Conditions at 5:45 p.m. were cloudy 62 degrees F 29% humidity with 3-5 mph east winds.

2016-05-09 Herbicide Application Sprayed GLBRC Main Site treatment G1 all replications (1, 2, 3, 4, 5), the inside perimeter bait crop,(outside 30' was sprayed to clean sprayer out) and CE study C2 plots for pre-emergence weed control and post plant burndown where corn was planted. Sprayed with Lexar at 3 qts/A and Roundup Power Max at 32 oz/A and ammonium sulfate at 3.4 lbs/A. Weather conditions at the start 3:40 p.m. were coudy 61 degrees F, 44% humidity with 5-10 mph south south east wind. Conditions at the end 5:30 p.m. were cloudy, 60 degrees F, 48% humidity, and 5-10 mph east wind.

2016-05-12 Fertilizer Application Applied fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Each treatment plot was divided into two areas, an unfertilized micro-plot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' micro-plot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. Weather conditions: 64 F cloudy and humid. Equipment Used: JD 5220 tractor with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Chafer stream bars were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi was used.

2016-05-12 Fertilizer Application Applied fertilizer in the GLBRC Main Site Treatment G10, MICRO-PLOT area only, all replications. Treatment G10 micro-plots were fertilized with 50 lbs N/A applied as 28% N fertilizer. The 28% fertilizer weighs 10.67 lbs / gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Each treatment plot was divided into two areas: fertilized micro-plot areas 15' wide located on the west side of treatment plots (G10R2, G10R3, G10R4, and G10R5) and G10R1 located on the east side of the treatment plot; unfertilized areas which were the remaining 76.86' on the east side of the plots (G10R2, G10R3, G10R4, and G10R5) and G10R1 located on the west side of treatment plot. The fertilized 15' micro-plot received 50 lbs N/A. The unfertilized 76.86' was NOT fertilized. Weather conditions: 64 F overcast and humid. Equipment Used: JD 5220 tractor with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Chafer stream bars were used spaced 15 inches apart with a total boom length of 30 feet. 20 psi was used. Purpose of Application: Supply nitrogen to the treatment plots.

2016-05-12 Fertilizer Application Fertilized LTER Cellulsic Biofuels Experiment (CE Study) treatment CE4 and CE6, all replications. Treatments CE4: (plots 110, 203, 302, 404); and CE6: (plots 111, 204, 310, 406). Plots received 50 lbs N/A with 17 gallons/A 28% N liquid fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Weather conditions: 64 F cloudy and humid. Equipment Used: JD 5220 tractor with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Chafer stream bars were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi was used.

2016-05-13 Fertilizer Application Fertilized GLBRC Main Site Switchgrass Fertility Study (SWF: N-rate), all treatments and all replications. Fertilizer applied as 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN) (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). The amounts of nitrogen applied were as follows: SWF1 = 0 lbs/A (108, 201, 307, 408); SWF2 = 25 lbs N/A (8gal/A) (104, 206, 308, 407); SWF3 = 50 lbs N/A (17 gal/A) (102, 202, 302, 406); SWF4 = 75 lbs N/A (25 gal/A) (105, 208, 305, 405); SWF5 = 100 lbs N/A (34 gal/A) (101, 205, 304, 404); SWF6 = 125 lbs N/A (42 gal/A) (107, 207, 301, 403); SWF7 = 150 lbs N/A (50 gal/A) (106, 204, 306, 402); SWF8 = 175 lbs N/A (59 gal/A) (103, 203, 303, 401). Weather Conditions: 9:00 a.m. sunny 51 degrees F, 74% humidity 4-10 mph south, south-west wind. 10:08 a.m. 60 degrees F 67 % humidity 2-10 south, south-west winds. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. The sprayer was controlled by a John Deere Rate Controller. Chafer stream bars were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer; 11 nozzles were used on the boom. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 17-40 psi. 1.0 Orifice's were used in the chafer stream bars for SWF2 (17psi.C1), SWF3 (40psi B2), SWF4 (40psi B1); and 1.8 orifice's were used in the chafer stream bars on SWF5 (30psi B3), SWF6 (30psi B2), SWF7 (20psi B1),and SWF8 (30psi B1).

2016-05-13 Fertilizer Application Applied 28% (28-0-0) nitrogen fertilizer at 17 gals/A (50.78 lbs N/A) to the switchgrass in field M3 at the Marshall Farm. One gallon of 28% fertilizer weighs 10.67 lbs. so 50.78 lbs N/A was applied. Weather conditions at start 1:15 p.m. partly cloudy, 58 degrees F. 69% humidity, wind out of the west 4-12 mph, Avg. 10 mph. Weather conditions at start 3:15 p.m. partly cloudy, 61 degrees F. 58% humidity, wind out of the west 4-10 mph, Avg. 8 mph. Time to complete the N application: 11:00 or 11:30 a.m. till 3:30 p.m.

2016-05-20 Herbicide Application Sprayed the GLBRC Main Site G2, G3, G4 treatments all replications, north 30 feet of north perimeter, east 60 feet of east perimeter, south 60 feet of south perimeter, 5 plots in rep 6 and behind the brown house with a post plant burndown application of Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 1:50 p.m. cloudy 67 degrees F, humidity was 39 % with 5-10 east winds. End of application 5:32 p.m. cloudy, 67 degrees F, humidity 40 %, with 5-10 east winds.

2016-05-20 Herbicide Application Sprayed the LTER CE Sutdy treatments C1 and C3 with a burndown application of Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 1:50 p.m. cloudy 67 degrees F, humidity was 39 % with 5-10 east winds. End of application 5:32 p.m. cloudy, 67 degrees F, humidity 40 %, with 5-10 east winds.

2016-06-03 Insecticide Application Sprayed LTER Main Site treatments, T1, T2, T6, N-Rate Study, Lysimeter field, and T8nt plots (treatment T2 was sprayed on June 2, 2016, All others sprayed June 3, 2016). Plots were sprayed with a fungicide (Prosaro), and an insecticide (Grizzly Z) tank mixture. Prosaro was applied at 8.2 oz/A with a NIS surfactant added at 4 oz/ 100 gallons of water carrier as a preventative measure against disease pressure. Grizzly Z was applied at 3.2 oz/A to control cereal leaf beetle larvae that were found in the plots. June 2, 2016 weather conditions, 6:30 p.m. partly sunny 75 degrees F, 45 % humidity, 6-10 mph west wind. 8:30 p.m. partly cloudy, 72 degrees F, 49 % humidity, 4-8 mph north west wind. June 3 weather conditions, 8:30 a.m. sunny, 62 degrees F, 86 % humidity, 1-3 mph north wind. 4:10 p.m. sunny, 80 degrees F, 40 % humidity, 2-6 mph south west wind.

2016-06-14 Fertilizer Application Applied phosphorus to GLBRC Main Site treatments G1: R2-R5, G2: R1, R2, R3, R5, and G3: R1-R5. Applied super phosphate 0-46-0 at 100 lbs/A on all plots listed except G3:R4 which received 120 lbs/A.

2016-06-29 Herbicide Application Sprayed the GLBRC Main Site G1, G2, G3, G4 treatments all replications all bait crop and the 4 treatments of the study on the north side of the drive by 40th st. with a post application of Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 9:15 a.m. sunny 62 degrees F, humidity was 70 % with 2-4 variable winds. End of application 2:00 p.m. sunny, 75 degrees F, humidity 44 %, with 3-5 south winds.

2016-08-02 Herbicide Application Sprayed LTER Main Site T3 plots all replication for post harvest / pre-plant burn down. All replications sprayed in this order (5, 4, 2, 1, 6, 3) with RoundUp Power Max at 32 oz/A, Ammonium Sulfate at 17 lbs/ 100 gal of carrier, and anti-foaming agent at 1 oz/ 100 gal of carrier. Time of application was 11:00 a.m. with weather conditions sunny 78 degrees F, 76% humidity with winds 3-5 mph out of the West. End time of application was 2:00 with weather conditions sunny 83 degrees F, 54% humidity with winds 4-6 mph of the West.

2016-08-18 Herbicide Application Sprayed LTER Main Site T6 plots all replications (1, 2, 3, 4, 5 and 6), N-Rate study and T8nt plots for post wheat harvest weed control and pre plant burn down before planting alfalfa in treatment T6. Plots sprayed with Roundup PowerMax herbicide at 44 oz/A, 17 lbs / 100 gallons/carrier of ammonium sulfate and 1 oz / 100 gallons/carrier of anti foaming agent. Time of application was from 11:15 a.m. - 6:40 p.m. Weather conditions at the beginning were partly sunny 76 degrees F, 81 % humidity and winds 2-6 mph south, weather conditions at the end were partly sunny 81 degrees F, 76 % humidity and winds 2-8 out of the south west.

2016-08-29 Herbicide Application Sprayed LTER Main Site T1 plots all replication for post harvest burn down. All replications sprayed in this order (5, 1, 4, 2, 3, 6) with RoundUp Power Max at 44 oz/A, 2,4-D Ester at 1 qt/A and Ammonium Sulfate at 17 lbs/ 100 gal of carrier, and anti-foaming agent at 1 oz/ 100 gal of carrier. Time of application was 12:50 p.m. with weather conditions mostly sunny 80 degrees F, 68% humidity with winds 4-7 mph out of the south west. End time of application was 3:00 with weather conditions mostly sunny 82 degrees F, 63% humidity with winds 4-6 mph of the south west.

2016-09-01 Herbicide Application Sprayed LTER Main Site T2 plots reps (5, 2, 3, 4, 2, 6) and lysimeter field for post harvest burndown. Plots sprayed with RoundUp Power Max at 44 oz/A, 2,4-D Ester at 1 qt/A and Ammonium Sulfate at 17 lbs/ 100 gal of carrier, and anti-foaming agent at 1 oz/ 100 gal of carrier. Time of application was 10:00 a.m. with weather conditions sunny 62 degrees F, 86% humidity with winds 3-6 mph out of the north east. End time of application was 2:20 p.m. with weather conditions sunny 73 degrees F, 58% humidity with winds 6-10 mph of the north west/north east.

2016-10-19 Herbicide Application Sprayed LTER Main Site T6 plots all replications (5, 4, 3, 2, 1 and 6), and N-Rate study. T6 was sprayed for post emergence weed control in alfalfa and N-Rate study was sprayed to control volunteer wheat. Plots sprayed with Roundup PowerMax herbicide at 32 oz/A, 17 lbs / 100 gallons/carrier of ammonium sulfate and 1 oz / 100 gallons/carrier of anti foaming agent. Time of application was from 11:10 a.m. - 3:42 p.m. Weather conditions at the beginning were sunny 62 degrees F, 78 % humidity and winds 4-7 mph west south west, weather conditions at the end were sunny 72 degrees F, 50 % humidity and winds 4-6 out of the east south east.

2016-07-21 Planting Broadcast seeded red clover in the LTER Biodiversity Study corn treatments B5 (114, 317, 312, 405), B8 (118, 214, 310, 408), B15 (112, 213, 301, 415). Clover was seeded with 15' gandy at 20 lbs/A over the top of the corn.

2016-09-01 Herbicide Application Sprayed LTER Main Site T2 plots reps (5, 2, 3, 4, 2, 6) and lysimeter field for post harvest burndown. Plots sprayed with RoundUp Power Max at 44 oz/A, 2,4-D Ester at 1 qt/A and Ammonium Sulfate at 17 lbs/ 100 gal of carrier, and anti-foaming agent at 1 oz/ 100 gal of carrier. Time of application was 10:00 a.m. with weather conditions sunny 62 degrees F, 86% humidity with winds 3-6 mph out of the north east. End time of application was 2:20 p.m. with weather conditions sunny 73 degrees F, 58% humidity with winds 6-10 mph of the north west/north east.

2017-03-03 Planting Frost seeded LTER Biodiversity study treatments b3 (111, 203, 306, 403), b6 (107, 207, 317, 406), b17 (116, 211, 302, 417), with Medium Red Clover at 12 lbs/A. Clover seed had a coating that was 34% of the seed weight. A pure live seed calculation was done to calculate amount of seed per acre needed to achieve the 12 lbs/A of pure live seed. The calculation equaled 20 lbs/A seeding rate to achieve the 12 lbs/A of pure seed (see papers attached). Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 22.5 inches. Gandy motor setting was 1.78 with the red metering wheels in and the gates on the Gandy were in the first position. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Start of application was 8:30 a.m. weather was sunny 19 degrees F 2-5 mph west wind. End of application 9:00 a.m. sunny 20 degrees F 5-7 mph north west wind. Total amount of seed added was 30 pounds with 14 pounds remaining at the end. 16 pounds of seed applied over .82 acres equals 19.3 lbs/A seeding rate. This should give close to a 10 lbs/A pure live seeding rate.

2017-04-24 Herbicide Application Sprayed LTER Main Site T2 plots reps (6, 5, 1, 4, 3, 2), T8nt plots, CE1, CE2, CE3, and lysimeter field for pre-plant burndown. Plots sprayed with RoundUp Power Max at 1 qt/A, 2,4-D Ester at 1 pt/A and Ammonium Sulfate at 17 lbs/ 100 gal of carrier, and anti-foaming agent at 1 oz/ 100 gal of carrier. Time of application was 11:36 a.m. with weather conditions sunny 63 degrees F, 26% humidity with winds 4-8 mph out of the east. End time of application was 4:30 p.m. with weather conditions sunny 71 degrees F, 14% humidity with winds 8-11 mph of the east south east.

2017-04-25 Herbicide Application Sprayed GLBRC main site perimeter bait crop, rep 6, behind the house, 4 treatments along 40th north of the drive and G1 all replications (5, 4, 3, 2, 1) for pre-plant burndown. Plots sprayed with RoundUp Power Max at 1 qt/A, 2,4-D Ester at 1 pt/A and Ammonium Sulfate at 17 lbs/ 100 gal of carrier, and anti-foaming agent at 1 oz/ 100 gal of carrier. Time of application was 9:45 a.m. with weather conditions sunny 59 degrees F, 69% humidity with winds 8-10 mph out of the south east. End time of application was 12:55 p.m. with weather conditions sunny 67 degrees F, 70% humidity with winds 5-10 mph of the south east.

2017-04-25 Herbicide Application Sprayed LTER main site Fertility Study both irrigated and non irrigated for pre-plant burndown. Plots sprayed with RoundUp Power Max at 22 oz/A, 2,4-D Ester at 1 pt/A and Ammonium Sulfate at 17 lbs/ 100 gal of carrier, and anti-foaming agent at 1 oz/ 100 gal of carrier. Start of application was 12:55 p.m. with weather conditions sunny 67 degrees F, 70% humidity with winds 5-10 mph of the south east. End of application was 2:15 p.m. with weather conditions sunny 69 degrees F, 53% humidity with winds 8-10.5 mph out of the south.

2017-05-10 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatments G5, G6, G7, and G9, all replications. All treatments were fertilized with 50 lbs N/A applied as 28% N fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Each treatment plot was divided into two areas, an unfertilzed microplot area 15' wide located on the west side of all the plots and the fertilized area which was the remaining 76.86' on the east side of the plot. The unfertilized 15' microplot received NO fertilizer. The fertilized 76.86' was fertilized at 50 lbs N/A applied in 3 passes of the sprayer. Conditions: 11:10 a.m. overcast 60 degrees F, 51% humidity, 4-5 mph south east wind. Conditions at end 1:38 p.m. overcast 68 degrees F 45 % humidity, 3-5 mph south west wind. Equipment Used: JD 5220 tractor with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Chafer stream bars were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Purpose of Application: Supply nitrogen to the treatment plots.

2017-05-10 Fertilizer Application Sprayed fertilizer in the GLBRC Main Site Treatment G10, MICROPLOT area only, all replications. Treatment G10 microplots were fertilized with 50 lbs N/A applied as 28% N fertilizer (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). 28% fertilizer weighs 10.67 lbs / gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Each treatment plot was divided into two areas: fertilized microplot areas 15' wide located on the west side of treatment plots [G10R2, G10R3, G10R4, and G10R5] and located on the east side of the treatment plot G10R1; unfertilized areas which were the remaining 76.86' on the east side of the plots [G10R2, G10R3, G10R4, and G10R5] and located on the west side of treatment plot G10R1. The fertilized 15' microplot received 50 lbs N/A. The unfertilized 76.86' was NOT fertilized. Conditions: 11:10 a.m. overcast 60 degrees F, 51% humidity, 4-5 mph south east wind. Conditions at end 1:38 p.m. overcast 68 degrees F 45 % humidity, 3-5 mph south west wind. Equipment Used: JD 5220 tractor with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and a John Deere Rate Controller. Chafer stream bars were used, spaced 15 inches apart. Purpose of Application: Supply nitrogen to the treatment plots.

2017-05-10 Fertilizer Application Fertilized LTER Cellulsic Biofuels Experiment (CE Study) treatment CE4 and CE6, all replications. Treatments CE4: (plots 110, 203, 302, 404); and CE6: (plots 111, 204, 310, 406). Plots received 50 lbs N/A with 17 gallons/A 28% N liquid fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 17 gal 28%/A. Weather conditions: 64 F cloudy and humid. Equipment Used: JD 5220 tractor with a pull type Top Air sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Chafer stream bars were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi was used.

2017-05-10 Herbicide Application Sprayed GLBRC Main Site G1 all replications (1, 2, 3, 4, 5), middle 30 ft on all three sides of perimeter bait crop, T8nt plots, CE3 plots (105, 207, 301, 403), LTER N-Rate Study for pre-emergence weed control where corn was planted with Lexar at a rate of 3 qts/A. Weather conditions at the start 3:00 p.m. were partly sunny 70 degrees F, 38% humidity with 3-5 mph wind out of west. Conditions at the end 5:10 p.m. were partly sunny, 69 degrees F, 41% humidity, and 2-4 mph winds out of the south. Equipment Used: JD 5220 tractor traveling 5.0 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Twin Turbo TeeJet 11003 nozzles were used, spaced 15 inches apart with a total boom length of 30 feet. 20 psi were used. Water applied at 20 gals/A, (187 L/ha), was used as the carrier.

2017-05-19 Fertilizer Application Fertilized GLBRC Main Site Switchgrass Fertility Study (SWF: N-rate), all treatments and all replications. Fertilizer applied as 28-0-0 which supplied 28% nitrogen in the form of Urea-Ammonium Nitrate (UAN) (CAS #57-13-6 Urea, #6484-52-5 Ammonium Nitrate). The amounts of nitrogen applied were as follows: SWF1 = 0 lbs/A (108, 201, 307, 408); SWF2 = 25 lbs N/A (8gal/A) (104, 206, 308, 407); SWF3 = 50 lbs N/A (17 gal/A) (102, 202, 302, 406); SWF4 = 75 lbs N/A (25 gal/A) (105, 208, 305, 405); SWF5 = 100 lbs N/A (34 gal/A) (101, 205, 304, 404); SWF6 = 125 lbs N/A (42 gal/A) (107, 207, 301, 403); SWF7 = 150 lbs N/A (50 gal/A) (106, 204, 306, 402); SWF8 = 175 lbs N/A (59 gal/A) (103, 203, 303, 401). Weather Conditions: 9:15 a.m. overcast 44 degrees F, 78% humidity 9-10 mph north east wind. 11:30 a.m. 49 degrees F 68 % humidity 9-10.5 mph north east winds. Equipment used: John Deere 5220 tractor pulling a Top-Air pull type sprayer equipped with a hydraulic centrifugal sprayer pump. A single pass of a boom type sprayer was used to broadcast spray and apply the correct amount of fertilizer. The sprayer was controlled by a John Deere Rate Controller. Chafer stream bars were spaced 15 inches apart along the 15 foot boom length extending from the center of the drive-line off to the right side of the sprayer; 11 nozzles were used on the boom. Different pressures, rpm, and speeds were used for the application so that the pressure range at the sprayer varied between 17-40 psi. 1.0 Orifice's were used in the chafer stream bars for SWF2 (17psi.C1), SWF3 (40psi B2), SWF4 (40psi B1); and 1.8 orifice's were used in the chafer stream bars on SWF5 (30psi B3), SWF6 (30psi B2), SWF7 (20psi B1),and SWF8 (30psi B1).

2017-06-08 Herbicide Application Sprayed the GLBRC Main Site G2, G3, G4 treatments all replications all bait crop, (except 30 ft of corn around perimeter that had already been sprayed), behind the house, rep 6 and the 4 treatments of the study on the north side of the drive by 40th st. with a post application of Roundup PowerMax, and ammonium sulfate. Roundup Power Max was applied at 22 oz/A and Ammonium Sulfate was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 8:00 a.m. sunny 58 degrees F, humidity was 79 % with 2-4 mph south south west winds. End of application 12:00 p.m. sunny, 71 degrees F, humidity 47 %, with 3-6 south, south west winds.

2017-06-08 Herbicide Application Sprayed LTER Main Site treatment T3 all replications (6, 3, 1, 2, 4, 5) for post-emergence weed control where corn was planted. Sprayed with Roundup Powermax at a rate of 22 oz/A, ammonium sulfate at 3.4 pounds/A and Dual II Magnum at a rate of 1.33 pts/A. Weather conditions at the start 12:50 p.m. were mostly sunny 74 degrees F, 44% humidity with4-6 mph west south west winds. Conditions at the end 3:12 p.m. were mostly sunny, 81 degrees F, 35% humidity, and 4-6 mph south west.

2017-06-09 Herbicide Application Sprayed LTER Main Site treatment T1 all replications (6, 3, 2, 5, 1, 4), and T2 all replications (6, 1, 4, 3, 2, 5) for post-emergence weed control where corn was planted. Sprayed with Roundup Powermax at a rate of 22 oz/A ( T1 rep 4 rate was increased to 32 oz/A due to the amount of grass in the plot), ammonium sulfate at 3.4 lbs/A and Lexar at 3 qts/A. Weather conditions at the start 9:00 a.m. were mostly sunny 62 degrees F, 74% humidity with 4-6 mph south west winds. Conditions at the end 3:30 p.m. were mostly sunny, 80 degrees F, 52% humidity, and 9.5-10 mph winds out of the south west.

2017-06-10 Herbicide Application Sprayed LTER Main Site Lysimeter field for post-emergence weed control where corn was planted. Sprayed with Roundup Powermax at a rate of 22 oz/A, ammonium sulfate at 3.4 lbs/A and Lexar at 3 qts/A. Weather conditions at the start 7:15 a.m. were mostly sunny 65 degrees F, 82% humidity with 4-6 mph south west winds. Conditions at the end 9:00 a.m. were mostly sunny, 68 degrees F, 72% humidity, and 4-6 mph winds out of the south west.

2017-06-23 Herbicide Application Sprayed the GLBRC Main Site G1 plots all replications (3, 2, 5, 4, 1) and the middle 30 ft of corn around the perimeter bait crop with a post application of Roundup PowerMax, and ammonium sulfate. Roundup Power Max was applied at 1 qt/A and Ammonium Sulfate was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 12:50 p.m. cloudy 70 degrees F, humidity was 84 % with 8-9.5 mph west winds. End of application 2:10 p.m. cloudy, 72 degrees F, humidity 77 %, with 6-8 mph west winds.

2017-07-06 Herbicide Application Sprayed the LTER CE Sutdy treatments C1, C2 and C3 and sprayed t8nt plots the north and south 8 feet headlands (due to corn was to tall to go into plot) with a post emerge application of Roundup PowerMax, and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 9:18 a.m. sunny 70 degrees F, humidity was 79 % with 2-3 mph south east winds. End of application 2:34 p.m. sunny, 86 degrees F, humidity 48 %, with 3-6 mph south west winds.

2017-07-06 Herbicide Application Sprayed GLBRC G2, G4 corn plots, 3 corn plots in rep 6, old productivity study and south and east outside 30' of perimeter bait crop with a post emerge application of Roundup PowerMax, Dual II Magnum and ammonium sulfate. Roundup Power Max (EPA Reg. No. 524-549) was applied at 1 qt/A, Dual II Magnum applied at 1.33 pint/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 9:18 a.m. sunny 70 degrees F, humidity was 79 % with 2-3 mph south east winds. End of application 2:34 p.m. sunny, 86 degrees F, humidity 48 %, with 3-6 mph south west winds.

2017-07-14 Herbicide Application Sprayed GLBRC G2, G4 corn plots, 3 corn plots in rep 6 and corn in the old productivity study with Liberty 280 SL and Ammonium sulfate. Liberty 280 SL was applied at 29 ounces/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Drop nozzles were used to spray down under canopy and avoid spraying the whorl of the corn. Weather conditions: At the start 12:00 p.m. partly cloudy 73 degrees F, humidity was 75 % with 4-6 mph north west winds. End of application 2:40 p.m. mostly sunny, 75 degrees F, humidity 62 %, with 6-8 mph west winds.

2017-07-19 Herbicide Application Sprayed GLBRC G3 and bait crop soybeans with First Rate and Roundup Powermax. First Rate was applied at .3 ounces/A, Roundup Powermax was applied at 32 oz/A, Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier and Non Ionic Surfactant was applied at 1 qt/100 gallons of carrier. Weather conditions: At the start 12:50 p.m. sunny 84 degrees F, humidity was 68 % with 3-5 mph west winds. End of application 3:12 p.m. sunny, 87 degrees F, humidity 63 %, with 5-6 mph west winds.

2017-07-21 Insecticide Application Sprayed GLBRC G3 and east and south inner perimeter bait crop soybeans with Dimethoate 400 EC. Dimethoate 400 EC was applied at 1pt/A to control spider mites. Weather conditions: At the start 1:50 p.m. sunny 84 degrees F, humidity was 58 % with 3-4 mph east winds. End of application 2:40 p.m. sunny, 87 degrees F, humidity 53 %, with 3-4 mph south west winds.

2018-05-07 Herbicide Application Sprayed GLBRC G1 corn plots, 1 corn plot (last one to west) in rep 6 and corn in the old productivity study (south half) with Roundup Powermax, Lexar and AMS. Roundup Powermax applied at 1qt/A, Lexar applied at 3qts/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 12:30 p.m. sunny 74 degrees F, humidity was 40.6 % with 5-10 mph north, north east End of application 2:30 p.m. sunny, 67 degrees F, humidity 28 %, with 5-10 mph west winds.

2018-05-08 Herbicide Application Sprayed LTER T2, T8nt plots, CE 3 and pesticide pad with Roundup Powermax, 2,4-D est Lv4 and AMS. Roundup Powermax applied at 22oz/A, 2,4-D lv4 ester at 1 pint/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 8:50 a.m. sunny 59 degrees F, humidity was 58.4 % with 5-9 mph south west. End of application 11:40 p.m. sunny, 68 degrees F, humidity 39.6 %, with 5-9 mph south west winds.

2018-05-08 Herbicide Application Sprayed GLBRC G2 plot 1 plot in rep 6, LTER N-Rate Study and Lysimeter field with Roundup Powermax, 2,4-D est Lv4 and AMS. Roundup Powermax applied at 22oz/A, 2,4-D lv4 ester at 1 pint/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 11:40 p.m. sunny, 68 degrees F, humidity 39.6 %, with 5-9 mph south west winds. End of application 3:10 p.m. sunny, 77 degrees F, humidity 28.9 %, with 5-9 mph north west winds.

2018-05-08 Herbicide Application Sprayed LTER T6 plots with Liberty 280 SL and 2,4-D est Lv4 and AMS. Liberty 280 SL applied at 1qt/A, 2,4-D lv4 ester at 1qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 3:45 p.m. sunny, 78 degrees F, humidity 27.5 %, with 3-9 mph west winds. End of application 6:10 p.m. sunny, 79 degrees F, humidity 26.3 %, with 3-6 mph north west winds.

2018-05-27 Herbicide Application Sprayed LTER Main Site treatment T2 with Valor. Valor was applied at 3.5 oz/A. Weather conditions: At the start 8:53 a.m. sunny 74 degrees F, humidity was 75 % with 3-6 mph south west. End of application 10:43 a.m. sunny, 83 degrees F, humidity 57 %, with 2-4 mph southwest winds.

2018-05-27 Herbicide Application Sprayed LTER Main Site treatment T1 with Valor. Valor was applied at 3.5 oz/A. Weather conditions: At the start 12:10 p.m. sunny 85 degrees F, humidity was 50.4 % with 3-5 mph south west. End of application 2:00 p.m. sunny, 89 degrees F, humidity 42 %, with 4-8 mph north west winds.

2018-06-06 Herbicide Application Sprayed LTER Main Site Lysimeter field with Valor and Roundup Powermax. Valor was applied at 3.5 oz/A Roundup Powermax applied at 22 oz/A. Weather conditions: At the start 12:40 p.m. partly sunny 68 degrees F, humidity was 50 % with 2-4 mph south west. End of application 2:40 p.m. sunny, 70 degrees F, humidity 46 %, with 2-4 mph south east winds.

2018-06-08 Herbicide Application Sprayed GLBRC Main Site treatment G2 with Dual II Magnum and Roundup Powermax. Dual II Magnum was applied at 1.33 pts/A Roundup Powermax 22 oz/A. Weather conditions: At the start 2:55 p.m. sunny 82 degrees F, humidity was 20 % with 2-4 mph south east. End of application 4:20 p.m. sunny, 80 degrees F, humidity 56 %, with 5-7 mph south winds.

2018-06-12 Herbicide Application Sprayed LTER Main Site treatment T6 with Select Max and Roundup Powermax. Select Max was applied at 9 oz/A Roundup Powermax 48 oz/A. Weather conditions: At the start 9:40 a.m. cloudy 66 degrees F, humidity was 90 % with 6-9 mph south east. End of application 1:07 p.m. cloudy, 70 degrees F, humidity 84 %, with 6-9 mph south east winds.

2018-06-28 Herbicide Application Sprayed LTER Main Site treatment T3 with Roundup Powermax and AMS. Roundup was applied at 1 qt/A and Ammonium Sulfate was applied at 3.4 lbs/A. Weather conditions: At the start 11:00 a.m. sunny 78 degrees F, humidity was 71.6 % with 4-6 mph north west winds. End of application 2:00 p.m. sunny, 82 degrees F, humidity 62 %, with 4-6 mph south west winds.

2018-07-11 Herbicide Application Sprayed sorghum planted in GLBRC rep 6 (1 plot) with Huskie and Atrazine 4F. Huskie was applied at 16 oz/A and Atrazine 4F was applied at 1qt/A (1 lb a.i.). Sorghum was right at the 12 inch labeled height for atrazine. Weather conditions: At the start 9:40 a.m. sunny 73 degrees F, humidity was 52.6 % with 4-6 mph East winds. End of application 10:40 a.m. sunny, 75 degrees F, humidity 46 %, with 5-7 mph East winds.

2018-07-11 Herbicide Application Sprayed LTER Soybeans in the N-Rate Study, CE 3, and T8nt plots with Roundup Powermax and AMS. Roundup was applied at 1 qt/A and Ammonium Sulfate was applied at 3.4 lbs/A. Weather conditions: At the start 2:00 p.m. sunny 81 degrees F, humidity was 40 % with 3-5 mph north east winds. End of application 4:20 p.m. sunny, 82 degrees F, humidity 38.6 %, with 5-7 mph north east winds.

2018-07-12 Herbicide Application Sprayed LTER Main Site treatment T1, T2 plots (T1R1, T1R6, T2R6, T2R5, T1R5, T2R2*,T2R3, T2R4, T1R4, T1R2, T1R3, T2R1) with Roundup Powermax and AMS. Roundup was applied at 1 qt/A and Ammonium Sulfate was applied at 3.4 lbs/A. Weather conditions: At the start 9:00 a.m. sunny 70 degrees F, humidity was 70.7 % with 4-6 mph south east winds. End of application 5:30 p.m. sunny, 81 degrees F, humidity 47.2 %, with 6-8 mph south east winds.

2018-07-25 Planting Seeded Medium Red Clover into corn plots in LTER Biodiversity Study treatments B3:(111, 203, 306, 403), B6:(107, 207, 317, 406), B15:(112, 213, 301, 415). Seeded red Clover at 12 lbs/A. Clover seed had a coating that was 34% of the seed weight. A pure live seed calculation was done to calculate amount of seed per acre needed to achieve the 12 lbs/A of pure live seed. The calculation equaled 20 lbs/A seeding rate to achieve the 12 lbs/A of pure seed. Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 22.5 inches. Gandy motor setting was 1.8 with the red metering wheels in and the gates on the Gandy were in the first position. Clover was applied directly over standing corn.

2018-07-25 Herbicide Application Comment: Sprayed LTER Lysimeter Field with Roundup Powermax and AMS. Roundup was applied at 1 qt/A and Ammonium Sulfate was applied at 3.4 lbs/A. Weather conditions: At the start 1:40 p.m. sunny 80 degrees F, humidity was 55 % with 7-9 mph north west winds. End of application 3:00 p.m. sunny, 81 degrees F, humidity 50.4 %, with 6-9 mph west winds.

2018-10-09 Herbicide Application Sprayed LTER T6 Plots with Roundup Powermax, 2,4-D ester Lv4 and AMS. Roundup Powermax applied at 2 qts/A, 2,4-D lv4 ester at 1 qt/A and Ammonium Sulfate (sprayable, CAS #7783-20-2) was applied at 17 lbs/100 gallons of carrier. Weather conditions: At the start 12:40 p.m. sunny, 81 degrees F, humidity 66.5 %, with 6-9 mph south west winds. End of application 3:15 p.m. partly cloudy, 84 degrees F, humidity 57.7 %, with 8-11 mph South winds.

2019-03-18 Planting Seeded LTER Main Site Treatment T4 all replications (5, 3, 2, 1, 6, 4), with Medium Red Clover at 12 lbs/A. Clover seed had a coating that was 34.29% of the seed weight. A pure live seed calculation was done to calculate amount of seed per acre needed to achieve the 12 lbs/A of pure live seed. The calculation equaled 31 lbs/A seeding rate to achieve the 12 lbs/A of pure seed (see papers attached). Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear C1 at 1600 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 22.5 inches. Gandy motor setting was ? with the red metering wheels in and the gates on the Gandy were in the first position. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Start of application was 6:30 a.m. weather was clear 20 degrees F 0 mph wind. End of application 10:30 a.m. sunny 30 degrees F 0 mph wind. Total amount of seed added was 450 pounds with 85 pounds remaining at the end. so 365 pounds of seed applied over 13 acres equals 28 lbs/A seeding rate.This equals almost 11 lbs/A pure live seed.

2021-07-01 Herbicide Application Spray weeds in soybeans.

2022-05-13 Fertilizer Application Fertilized LTER Main Site winter wheat treatment T3 all replications (5,4,2,1,3 and 6), Plots were fertilized with 10 gallons per acre of (28-0-0). 28-0-0 weighs 10.67 lbs/gallon so 30 lbs of N/A was applied. Weather conditions 11:30 a.m. were clear 83.3 degrees F, 37% humidity 8 mph Southwest wind. Application was completed using the John Deere 5220 Tractor and the Top Air 30ft sprayer. The sprayer was fitted with Chafer stream bars set to 1.0 orifice setting traveling an average of 5.5 mph.

2022-05-13 Fertilizer Application Fertilized LTER winter wheat N-Rate Study treatments 6-9. Only plots F6 to F9 were fertilized with 28% U.A.N. nitrogen fertilizer at the following rates to finish N application for the season. F6 at 13.4 gal/A =40 lb N, F7 at 20.1 gal/A = 60 lb N, F8 at 26.8 gal/A = 80 lb N and F9 at 33.5 gal/A = 100 lb N, Applied with Top Air Sprayer equipped with chafer streamer bars with 1.0 orifice. Do to low volume only half the boom was on and every 15 foot plot was covered individually. Conditions starting at 2:30 p.m. clear, 84 degrees F 37% humidity 8-9 mph Southwest winds. On 05/17/2022 the F5 remaining nitrogen was applied. F5 plots were fertilized with 28% U.A.N. nitrogen fertilizer at 6.7 Gallons per Acre, applying 20 lb N per Acre. Do to low volume only half the boom was on and every 15 foot plot was covered individually. Teejet 3 stream 015 green tips were used to apply the low volume evenly. Conditions starting at 3:30 p.m. clear, 64 degrees F 41% humidity 10-14 mph Northwest winds. Application was completed using the John Deere 5220 Tractor and the Top Air 30ft sprayer.

2022-05-16 Fertilizer Application Fertilized LTER Cellulsic Biofuels Experiment (CE Study) treatment CE4 and CE6, all replications. Treatments CE4: (plots 110, 203, 302, 404); and CE6: (plots 111, 204, 310, 406). Plots received 50 lbs N/A with 16.7 gallons/A 28% N liquid fertilizer. 28% fertilizer weighs 10.67 lbs /gallon so the 50 lbs N/A worked out to 16.7 gal 28%/A. Weather: 68.7 degrees F/ 8 mph wind speed ,West Wind/ 47% relative humidity.

2022-05-09 Fertilizer Application Fertilized LTER Main Site winter wheat treatment Lysimeter Field. The Lysimeter Field was fertilized with 20 gallons per acre nitrogen and sulfur fertilizer blend.

2022-05-16 Fertilizer Application Fertilized LTER Main Site winter wheat treatment Lysimeter Field. The Lysimeter Field was fertilized with 20 gallons per acre nitrogen providing 60 lb/A of nitrogen. Weather: 68.7 degrees F/ 8 mph wind speed ,West Wind/ 47% relative humidity.

2022-05-18 Fertilizer Application T6 plots were fertilized with 28% U.A.N. nitrogen fertilizer at 17 Gallons per Acre, applying 50 lb N per Acre. T6 Rep (1, 3, 4, 2, 5 and 6) Conditions starting at 10 a.m. cloudy, 51 degrees F 87% humidity 7 mph East winds. Application was completed using the John Deere 5220 Tractor and the Top Air 30ft sprayer using stream bars.

2022-05-19 Fertilizer Application Fertilized GLBRC Main Site Switchgrass Fertility Study (SWF: N-rate), all treatments and all replications. Fertilizer applied as 28-0-0 which supplied 28% nitrogen The amounts of nitrogen applied were as follows: SWF1 = 0 lbs/A (108, 201, 307, 408); SWF2 = 25 lbs N/A (8.4 gal/A) (104, 206, 308, 407); SWF3 = 50 lbs N/A (16.7 gal/A) (102, 202, 302, 406); SWF4 = 75 lbs N/A (25.1 gal/A) (105, 208, 305, 405); SWF5 = 100 lbs N/A (33.5 gal/A) (101, 205, 304, 404); SWF6 = 125 lbs N/A (41.8 gal/A) (107, 207, 301, 403); SWF7 = 150 lbs N/A (50.2 gal/A) (106, 204, 306, 402); SWF8 = 175 lbs N/A (58.6 gal/A) (103, 203, 303, 401). Weather Conditions: 9:00 am, partly cloudy, 74.8 degrees F, 11 mph Southwest wind, 66.1% humidity. A single pass from 30' top air sprayer using chafer stream bars was used. Half of sprayer was turned off to get 15'. SWF2 and SWF3 were applied using the Teejet 015 green SJ3 tips. The sprayer was controlled by a John Deere Rate Controller.

2022-05-19 Herbicide Application Sprayed G1 G2 G3 all Reps, 4 blocks in Rep 6 as well as the bait crop. Sprayed CE1 CE2 CE3 and the Bait crop. Brown House section were sprayed on 05,20,2022 at 7:30 A.M. Sprayed Roundup Powermax at 32 ounces/acre, Ammonium Sulfate at 3.4lbs/acre, and a small amount of de-foaming agent. Rate of spray was 20gal/acre on JD rate controller. Weather conditions on 05, 19 Sunny 72 degrees F, Winds 0-5 mph out of the Southwest. Weather conditions on 05, 20 Sunny 65.5 degrees F, Winds 10.3 mph out of the Southwest 88.5% Relative Humidity REI: 4 hours.

2022-05-25 Herbicide Application Sprayed G2, G3, & CE3 all Reps, 1 soybean block in Rep 6 as well as the soybean bait crop. Sprayed Dual II Magnum at 1.33 pints/acre, and a small amount of de-foaming agent. Rate of spray was 20 gal/acre on JD rate controller. Weather conditions: cloudy, light sprinkle/rain, 55.6 degrees F, Winds 9 mph out of the East 80.5% Relative Humidity REI: 24 hours.

2022-06-01 Fungicide Application Applied fungicide to T2 Reps(5,3,2,4,1 and 6) as well as the Resource Gradient Study and The Lysimeter field East and Southeast of the T2 rep 6 plot. REI of 12 Hrs 11:50 P.M. on 06/01/22 13.6 oz per acre of Prosaro Pro 400SC was applied with water at a rate of 20 gap per acre. Weather conditions at 9 A.M. were overcast with 5.5 mph or slower Northwest winds. The Temperature was 70.4 degrees F and the relative humidity was 85.2%. Application was completed using the 30ft top air sprayer and the John Deere 5220 tractor. The sprayer was fitted with Turbo Twin jet 1103 nozzles.

2022-06-02 Fungicide Application Applied fungicide to T1 Reps(5,6,1,4,3 and 2) as well as T3 Reps (1, 2, 3, 6, 5 and 4) REI of 12 Hrs 3:30 A.M. on 06/03/22 13.6 oz per acre of Prosaro Pro 400SC was applied with water at a rate of 20 gap per acre. Weather conditions at 11 A.M. were sunny with 3.2 mph or slower Northwest winds. The Temperature was 65.4 degrees F and the relative humidity was 73.2%. Application was completed using the 30ft top air sprayer and the John Deere 5220 tractor. The sprayer was fitted with Turbo Twin jet 1103 nozzles.

2022-06-09 Fungicide Application Applied fungicide to T2 All Reps, T1 All Reps, Resource Gradient Study, and the Lysimeter field East of the T2 plot. REI of 12 Hrs for T2 all reps and T1 Reps 1,2,3,4, and 6 @ 6:30 A.M. on 06/10/22 and REI of 12 Hrs for T1 R5 Resource Gradient Study and Lysimeter Field 11:30 PM on 06/10/2022 7.3 oz per acre of Sphaerex was applied with water at a rate of 20 gap per acre. Weather was 70 degrees F with 65%humidity. Wind was 6 to 8 MPH on 06/09 and 0 MPH on 06/10 out of the West or Southwest. Application was completed using the 30ft top air sprayer and the John Deere 5220 tractor. The sprayer was fitted with Turbo Twin jet 1103 nozzles.

2022-03-04 Planting Seeded LTER Main Site Treatment T4 all replications (5, 3, 2, 4) on 03/04/2022, and (1,6) on 03/05/2022 with Medium Red Clover at 12 lbs/A. (PLS of 51.75 makes the new target 23.19 lbs/A) Actual application was 23.66 lbs/A making the application rate 12.24 lbs/A. A pure live seed calculation was done to calculate amount of seed per acre needed to achieve the 12 lbs/A of pure live seed. The calculation equaled 23 lbs/A seeding rate to achieve the 12 lbs/A of pure seed (see papers attached). Clover was seeded with John Deere 5220 tractor traveling at 5.0 mph, gear B3 at 2000 rpm’s, and Gandy Air Seeder (15 ft boom length) equipped with 8 deflectors spaced 22.5 inches. Gandy motor setting was 2 with the red metering wheels in and the gates on the Gandy were in the first position. Boom height was 23 inches from the ground, bottom of the defectors were 20 inches from the ground. Start of 1st application was 7:00 a.m. on 03/04/2022 weather was clear 18 degrees F 0 mph wind. End of application 10:30 a.m. sunny 29 degrees F 0 mph wind. Start of 2nd application was 7:10 a.m. on 03/05/2022 weather was clear 34 degrees F 0 mph wind. End of application 8:15 a.m. sunny 35 degrees F 0 mph wind.

2022-03-22 Fertilizer Application Fertilized LTER Main Site winter wheat treatment T1 all replications (5,6,4,3,1, and 2), Plots were fertilized with 20 gallons per acre of (28-0-0). 28-0-0 weighs 10.67 lbs/gallon so 59.75 lbs of N/A was applied. Weather conditions 10 a.m. were cloudy 42 degrees F, 79.3% humidity 13 mph East wind. Conditions 1 p.m. cloudy and scattered showers, 43.3 degrees F 83.5% humidity 13 mph East winds. Application was completed using the John Deere 5220 Tractor and the Top Air 30ft sprayer. The sprayer was fitted with Chafer stream bars set to 1.0 orifice setting traveling an average of 6 mph. Applications in Rep 1,2,3,and 4 on the South headlands by the REX shelter sites was completed using one 15 ft pass and 1 to 2 30ft passes traveling east and west. This avoided driving in the shelter locations while applying the 20 gal/A.

2022-03-28 Fertilizer Application Fertilized LTER Main Site winter wheat treatment T8 all replications (1,2,3, and 4), Plots were fertilized with 20 gallons per acre of (28-0-0). 28-0-0 weighs 10.67 lbs/gallon so 59.75 lbs of N/A was applied. Weather conditions 1:30 p-.m. were clear 28 degrees F, 44.8% humidity 8 mph West wind. Application was completed using the John Deere 5220 Tractor and the Top Air 30ft sprayer. The sprayer was fitted with Chafer stream bars set to 1.0 orifice setting traveling an average of 5.5 mph.

2022-04-29 Herbicide Application Sprayed LTER Main Site Treatment T3 all replications (6, 1, 2,4,5and 3), for post emergence weed control in winter wheat. Plots sprayed with a tank mix of Ammonium Sulfate at 3 lb/A, Powerflex HL at 2 oz/A, and Non-ionic surfactant at 0.5% v/v T3 Time of application 9:30 a.m. to 12:50 p.m. 24 hour reentry interval. Equipment used: JD 5220 tractor traveling 5 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller.

2022-04-29 Herbicide Application Sprayed LTER Main Site Lysimeter field, for post emergence weed control in winter wheat. Plots sprayed with a tank mix of Ammonium Sulfate at 1 lb/A, Powerflex HL at 2 oz/A, Husky at 13.5 oz/A and Non-ionic surfactant at 0.25% v/v . Time of application was 1:50 p.m. - 2:30 p.m. 24 hour reentry interval. Sprayed LTER Main Site Resource Gradient all replications (Irrigated and Non-Irrigated), for post emergence weed control in winter wheat. Plots sprayed with a tank mix of Ammonium Sulfate at 1 lb/A, Powerflex HL at 2 oz/A, Husky at 13.5 oz/A and Non-ionic surfactant at 0.25% v/v (2 qt/100 gal carrier). RGE Time of application 12:45 p.m. to 1:45 p.m. 24 hour reentry interval. Weather Conditions were 55 degrees F, Humidity was 27%, and wind was 10 mph out of the Southeast. Equipment used: JD 5220 tractor traveling 5 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller. Equipment used: JD 5220 tractor traveling 5 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller.

2022-04-28 Herbicide Application Sprayed LTER Main Site Resource Gradient all replications (Irrigated and Non-Irrigated), for post emergence weed control in winter wheat. Plots sprayed with a tank mix of Ammonium Sulfate at 1 lb/A, Powerflex HL at 2 oz/A, Husky at 13.5 oz/A and Non-ionic surfactant at 0.25% v/v (2 qt/100 gal carrier). RGE Time of application 12:45 p.m. to 1:45 p.m. 24 hour reentry interval. Weather Conditions were 45.4 degrees F, Humidity was 40.6%, and wind was 6 to 8 mph out of the East. Equipment used: JD 5220 tractor traveling 5 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller.

2022-04-28 Herbicide Application Sprayed LTER Main Site Treatment T1 all replications (5, 1, 2,3,4and 6) and T8NT all replications, for post emergence weed control in winter wheat. Plots sprayed with a tank mix of Ammonium Sulfate at 1 lb/A, Powerflex HL at 2 oz/A, Husky at 13.5 oz/A and Non-ionic surfactant at 0.25% v/v (2 qt/100 gal carrier). T1 and T8NT Time of application was 1:50 p.m. - 7:00 p.m. 24 hour reentry interval. Weather Conditions were 45.4 degrees F, Humidity was 40.6%, and wind was 6 to 8 mph out of the East. Equipment used: JD 5220 tractor traveling 5 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller.

2022-04-21 Herbicide Application Sprayed LTER Main Site Treatment T2 all replications (5, 4, 3,1,6and 2), for post emergence weed control in winter wheat. Plots sprayed with a tank mix of Ammonium Sulfate at 1 lb/A, Powerflex HL at 2 oz/A, Husky at 13.5 oz/A and Non-ionic surfactant at 0.25% v/v (2 qt/100 gal carrier). Time of application was 4:55 p.m. - 7:40 p.m. Three passes in the West end of Rep 2 remain unsprayed. Weather conditions 4:55 p.m. were clear, temperature was 62°F, 45% humidity 10-12 mph West wind. Weather conditions 7:45 p.m. were clear, temperature was 62.2°F, 42% humidity 10-13 mph West wind. Equipment used: JD 5420 tractor traveling 5 mph with a pull type Top Air Sprayer equipped with a hydraulic centrifugal sprayer pump and John Deere Rate Controller.

2022-05-05 Fertilizer Application Fertilized LTER Main Site winter wheat treatment T2 all replications (5,3,4,1,6 and 2) and T1 all replications (5, 1, 4, 1, 3, and 6). T8nt 1 2 3 4 Plots were fertilized with 27 gallons per acre nitrogen and sulfur fertilizer blend. 28-0-0 N at 17.92 gallon per acre; 11-0-0-24 at 9.47 gallon per acre; This tank mix application applied approximately 65 pounds of nitrogen per acre and 25 pounds of sulfur per acre. Application was started at 1:30 p.m. and completed by 5:30 p.m. Weather conditions 1:30 p.m. were cloudy 57 degrees F, 63.6% humidity 5 mph East wind. Application was completed using the John Deere 5220 Tractor and the Top Air 30ft sprayer. The sprayer was fitted with Chafer stream bars set to 1.0 orifice setting traveling an average of 6 mph. Applications in Reps containing REX shelter sites was completed using one 15 ft pass on the south side of the REX plots and 1 to 30ft passes traveling east and west on the north side of the REX plots. This avoided driving in the REX shelter locations while applying the fertilizer to the plots.

2022-05-19 Fertilizer Application Interaction plots 1, 5, 7, 10, 12, 14, 15, and 16 were fertilized with 28% U.A.N. nitrogen fertilizer at 16.8 Gallons per Acre, applying 50 lb N per Acre. Conditions starting at 10 a.m. cloudy, 57.2 degrees F 67% humidity 12mph southwest winds. Application was completed using the John Deere 5220 Tractor and the Top Air 30ft sprayer using stream bars.

2022-05-19 Herbicide Application Sprayed G1 G2 G3 all Reps, 4 blocks in Rep 6 as well as the bait crop. Sprayed CE1 CE2 CE3 and the Bait crop. Brown House section were sprayed on 05,20,2022 at 7:30 A.M. Sprayed Roundup Powermax at 32 ounces/acre, Ammonium Sulfate at 3.4lbs/acre, and a small amount of de-foaming agent. Rate of spray was 20gal/acre on JD rate controller. Weather conditions on 05, 19 Sunny 72 degrees F, Winds 0-5 mph out of the Southwest. Weather conditions on 05, 20 Sunny 65.5 degrees F, Winds 10.3 mph out of the Southwest 88.5% Relative Humidity REI: 4 hours.

2022-06-17 Herbicide Application Sprayed G1 all Reps CE 1 nd CE2 , 2 corn blocks in Rep 6 as well as the bait crop corn. Applied Interline at 22 ounces/acre, Acuron at 2 qt/ acre, Ammonium Sulfate at 3.4lbs/acre, and a small amount of de-foaming agent. Rate of spray was 20gal/acre on JD rate controller. Weather conditions on 06, 17 Sunny 65 degrees F, Winds 5.5 mph out of the West 75.5% Relative Humidity REI: 12 hours.

2022-07-07 Herbicide Application Sprayed GLBRC main site G3 Reps 4,5,3,2, and 1 as well as Rep 6 / Bait Crop soybeans. LTER CE study 105, 207, 301, and 403 were also sprayed. Application of Enlist at 2 pints/acre, Interline (Generic Liberty) at 2 pints/acre, Ammonium Sulfate at 3lbs/acre, and a small amount of de-foaming agent. Rate of spray was 20gal/acre on JD rate controller. Weather conditions at the beginning 12:50pm: 78.3 degrees F, 2.93 mph northwest wind, 65.3% relative humidity, and partly cloudy. Weather conditions at the end 3:00 pm: 83 degrees F, 2.65 mph northwest wind, 56.1% relative humidity, and partly cloudy. REI: 48 hours. Application was completed using the 30ft top air sprayer and the John Deere 5220 tractor.

2022-07-06 Mechanical Weed Control Mowed in between poplar columns in LTER T5 (Reps 4, 3, 2, 1, and 6) main plots. This was done to control undesired plant growth. Mowed in the South to North direction. Equipment used was John Deere 5320 tractor and John Deere 6' mower (brush hog). T5R4 mowed afternoon of 7/1/22 T5 Reps 3, 2, and 1 mowed 7/5/22 T5R6 mowed 7/6/22 T5R5 not yet mowed, waiting to remove black locus

2022-09-30 Herbicide Application Herbicide application to Lysimeter field, Resource Gradient Study, and T8nt plots. A tank mix of Round Up power max at 44 oz per acre (1.5 lbs per acre), 2-4D at 1 Quart per acre (3.325 pounds per acre), and Ammonium Sulfate at 3.4 lbs per acre were applied as a burndown. Rate of spray was 20gal/acre on JD rate controller. Application began at 12:30 pm, weather conditions at the time were: 58.5°F, wind speed 1.21 mph, wind direction East, relative humidity 62.5%, and sunny. Application concluded at 4:00 pm, weather conditions at the time were: 64.3°F, wind speed 1.03 mph, wind direction Northeast, relative humidity 49.2%, and sunny. REI: 12 hours. Application was completed using the 30ft top air sprayer and the John Deere 5220 tractor.

2022-10-03 Herbicide Application Herbicide application to T2 plots (reps 4, 3, 1, 2, 5, 6). A tank mix of Round Up power max at 44 oz per acre (1.5 lbs per acre), 2-4D at 21.3 oz per acre (0.63 pounds per acre), and Ammonium Sulfate at 3.4 lbs per acre were applied as a burndown. The rate of 2-4D was decreased due quantity available on hand. Rate of spray was 20gal/acre on JD rate controller. Application began at 1:30 pm, weather conditions at the time were: 62.3°F, wind speed 1.00 mph, wind direction East, humidity 40.3% Application concluded at 5:30 pm, weather conditions at the time were: 68.7°F, wind speed 4.42 mph, wind direction Northeast, relative humidity 26.6%, and sunny. REI: 12 hours. Application was completed using the 30ft top air sprayer and the John Deere 5220 tractor. Application in T2R6 was not completed same day, ran out of product. Remainder of T2R6 was sprayed next day, 10/4/22 without 2-4D.

2022-10-04 Herbicide Application Herbicide application to T1 plots (reps 4, 1, 5, 2, 6). Round Up power max at 44 oz per acre (1.5 lbs per acre) and Ammonium Sulfate at 3.4 lbs per acre were applied as a burndown. 2-4D was not applied to T1 plots because of the low perennial weed pressure and the upcoming tillage. Rate of spray was 20gal/acre on JD rate controller. Application began at 12:20 pm, weather conditions at the time were: 64.4°F, wind speed 4.56 mph, wind direction West, humidity 43.6% Application concluded at 4:30 pm, weather conditions at the time were: 70.5°F, wind speed 6.06 mph, wind direction West, relative humidity 29.0%, and sunny. REI: 4 hours. Application was completed using the 30ft top air sprayer and the John Deere 5220 tractor. Application in T1R6 was not completed same day, ran out of product. Remainder of T1R6 was sprayed next day, 10/5/22. Application began with the remainder of T2R6 from the previous application.

2023-05-30 Herbicide Application Sprayed GLBRC G2, G3 plots (Reps 5, 4, 3, 2, 1), and the Bait Crop with Interline. Spray rate was 20 gal/A with Interline rate at 35.5 oz/A and 3 lbs/A Ammonium Sulfate. Application began at 12:00 PM and concluded at 3:30 PM. Weather at 12:00 PM Temperature: 82.9°F Wind Direction: South Wind Speed: 2.4 MPH Relative Humidity: 37.2% Weather at 3:30 PM Temperature: 86.6°F Wind Direction: South Wind Speed: 6.5 MPH Relative Humidity: 31.7%

2023-05-31 Herbicide Application Sprayed T2 all replications (6, 1, 4, 3, 2, and 5) for pre-emergence weed control where corn was planted. Sprayed with Roundup PowerMax 3 at a rate of 21.5 oz/A, ammonium sulfate at 1.7 lbs/A, and Acuron at 2.5 qts/A. REI is 24 hours, safe re-entry 2:00 PM 6/1/23 Ran out of product in T2R5, short a pass and half along the west edge of the plot. The plot was finished later in the day while spraying T1 plots. Application began at 9:00 am and concluded at 2:00 pm. Weather conditions at 9:00 am were: Temperature: 74.1°F Wind Direction: Southeast Wind Speed: 3.7 MPH Relative Humidity: 66.7% Weather conditions at 2:00 pm were: Temperature: 86.3°F Wind Direction: Southeast Wind Speed: 6.6 MPH Relative Humidity: 32.1%

2023-05-31 Herbicide Application Sprayed T1 all replications (5, 1, 4, 2, 3, and 6) for pre-emergence weed control where corn was planted. Sprayed with Roundup Powermax 3 at a rate of 22 oz/A, ammonium sulfate at 1.7 lbs/A, and Acuron at 2.5 qts/A. REI is 24 hours, safe re-entry 7:00 PM 6/1/23 Began application by finishing remaining passes in T2R5. Ran out of product in T1R6, short last pass on West edge of plot. Application began at 3:45 pm and concluded at 7:00 pm. Weather conditions at 3:45 pm were: Temperature: 87.6°F Wind Direction: South Wind Speed: 7.3 MPH Relative Humidity: 26.2% Weather conditions at 7:00 pm were: Temperature: 88.3°F Wind Direction: Southeast Wind Speed: 5.1 MPH Relative Humidity: 29.0%

2023-06-01 Herbicide Application Sprayed LTER Resource Gradient (Rainfed and Irrigated), GLBRC G1 all reps (1, 4, 5, 2, and 3), T8nt all reps, GLBRC Bait Crop corn, CE Study Corn (CE1 and CE3), and currently planted area of the Lysimeter field for pre-emergence weed control where corn was planted. Sprayed with Roundup PowerMax 3 at a rate of 22 oz/A, ammonium sulfate at 1.7 lbs/A, and Acuron at 2.5 qts/A. Application began at 11:30 am and concluded at 5:05 pm. REI is 24 hours, safe re-entry time is 5:05pm Friday 6/2/23 safe re-entry by treatment: Resource Gradient: 12:55 pm Friday 6/2/23 CE Study corn: 1:25 pm Friday 6/2/23 GLBRC G1: 3:05 pm Friday 6/2/23 Lysimeter field corn: 4:50pm Friday 6/2/23 GLBRC Bait Crop corn: 5:05pm Friday 6/2/23 Weather conditions at 11:30 am: Temperature: 83.7°F Wind Direction: East Wind Speed: 3.01 mph Relative Humidity: 46.9% Weather conditions at 5:05 am: Temperature: 88.4°F Wind Direction: Southeast Wind Speed: 6.14 mph Relative Humidity: 31.8%

2023-06-06 Herbicide Application Sprayed LTER Main Site treatment T3 all replications () for early post-emergent weed control where corn was planted. Sprayed with tank mix of Dual II Magnum at a rate of 1.33 pts/A, Roundup PowerMax at 22 oz/A, and ammonium sulfate at 17lbs/100 gallons (3.4 lbs/A). Application began at 10:25 am and concluded at 2:30 pm. REI is 24 hours, safe re-entry. 2:30 PM 6/7/23. Weather conditions at 10:30 am were: Temperature: 65.8 °F Wind Speed: 4.9 mph Wind Direction Northeast Relative Humidity: 69.2% Weather conditions at 2:30 am were: Temperature: 76.8 °F Wind Speed: 7.6 mph Wind Direction Northeast Relative Humidity: 39.9%

2023-06-08 Herbicide Application Sprayed G2 (Rep 5, 2, 3, 1, and 4) and G3 (Rep 3, 5, 2, 4, and 1) for pre-emergent weed control Sprayed Moccasin II Plus (generic Dual II Magnum) at 1.33 pints/acre and Roundup PowerMax at 22 oz/A, AMS at 9 lbs/ 100 gallons, and a small amount of de-foaming agent. Rate of spray was 20 gal/acre on JD rate controller. Application began at 1:00 pm and concluded at 3:50 pm. Safe re-entry at 3:50 pm Friday June 6, 2023. Pre-emergent herbicide was applied immediately after planting Soybeans (G2) and the following day after planting Sorghum (G3). Weather Conditions at 1:00 pm were: Temperature: 70.8°F Wind Speed: 9.5 mph Wind Direction: Northwest Relative Humidity: 27.7% Weather Conditions at 3:50 pm were: Temperature: 73.4°F Wind Speed: 9.3 mph Wind Direction: Northwest Relative Humidity: 23.6% Wind speed was variable throughout application, with average wind speed fluctuating between 4.9 mph and 9.5 mph. Average speed never exceeded 10 mph.

2023-06-09 Herbicide Application Sprayed LTER CE study CE2 (112, 210, 312, 402), and GLBRC bait crop planted soybeans for pre-emergent weed control Sprayed Moccasin II Plus (generic Dual II Magnum) at 1.33 pints/acre and Roundup PowerMax at 22 oz/A, AMS at 9 lbs/ 100 gallons, and a small amount of de-foaming agent. Rate of spray was 20 gal/acre on JD rate controller. Application began at 11:00 am and concluded at 11:30 am. Safe re-entry at 11:30 Saturday June 10, 2023. Weather Conditions at 11:00 am were: Temperature: 67.3°F Wind Speed: 5.8 mph Wind Direction: Northeast Relative Humidity: 46.2% Weather Conditions at 11:30 am were: Temperature: 67.9°F Wind Speed: 4.3 mph Wind Direction: Northwest Relative Humidity: 44.9%

2023-07-21 Herbicide Application Sprayed Liquid Fence Deer and Rabbit Repellent around perimeters of GLBRC G1 plots (1, 3, 5, 2, and 4), GLBRC Bait Crop, LTER T8nt plots, and LTER T1R1 (excluding REX area). Product has no REI. Application done to reduce deer damage as corn tassels.

2023-08-31 Herbicide Application Herbicide application to GLBRC G2 (reps 3, 2, 5, 4, 1), Bait Crop area (soybean), and brown house field, LTER CE Study soybeans CE2 (112, 210, 312, 402), and unplanted area of the Lysimeter field. RoundUp PowerMax 3 at 38.5 oz per acre and Ammonium Sulfate at 3.4 lbs per acre were applied post-emergence to control grass weeds. Rate of spray was 20gal/acre on JD rate controller. REI is 4 hours, safe re-entry on Thursday 8/31/23 at 8:00 PM Weather at 10:50 am: Temperature: 63.1°F Wind Direction: East Wind Velocity (3m average): 4.31 mph Relative Humidity: 61.2% Weather at 4:00 pm: Temperature: 73.6°F Wind Direction: East Wind Velocity (3m average): 2.43 mph Relative Humidity: 30.8%

2024-04-25 Herbicide Application Terminate cover crop and weed in preparation for corn planting. Wind 7.3 mph from NE, 56.8 F.

2024-04-25 Herbicide Application Terminate weeds in pre emergent oats and barley. Wind 3.8 mph E 53F.

2024-04-26 Herbicide Application Apply herbicide to suppress annual rye grass in wheat and barley. Only the south edge of A3W was treated. Wind 11.4 mph from SE, 47.4 F.

2024-04-26 Planting Broadcast soybeans at 130k and 180k seeds per acre and incorporate. Drill soybeans 137k seeds per acre and planting depth of 1.25 inches. Treatments according to Soybean Planting Method map. Brook used Monosem for other treatments.

2024-05-01 Fertilizer Application Apply 28% UAN ahead of corn planting bringing total nitrogen to 150 lbs. per acre.

2024-05-02 Herbicide Application Apply preemergent burndown and residual herbicide to soybeans in BAU2, ASP5, IP4, A1 south, B1 south, and B0. Wind 9 mph from SE, 72.2 F. Cleanout was done in IP4.

2024-05-02 Herbicide Application Spray wheat, oats, and rye to suppress mayweed chamomile and other weeds. Wind 6.7 mph from SE, 75.8 F.

2024-05-31 Planting Fill in rest of field with DKC55-53 and MN13 corn. Spray residual herbicide program on corn. Wind 6.4 mph SE, 75.8F.

2024-05-31 Fungicide Application Treat oats for cereal leaf beetle and fungus. Wind 2.8 mph S, 77.1 F.

2024-06-11 Herbicide Application Apply herbicide in soybeans. Wind 4.4 mph from W, 71.9F.

2024-06-19 Fungicide Application Apply fungicide to oat and spring barley plots. Wind 5.1 mph from S, 90.2F.